(Linear) (Six-base) MAPSORT of: KIBB7751.seq  Check: 9577  from: 1  to: 1335



KIBB7751.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:40  ..

AclI AA'CG_TT

Cuts at:      0     497    1335 
   Size:        497     838
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     461    1057    1335 
   Size:        201     260     596     278

  Fragments arranged by size: 

                 596     278     260     201
 
AvaI C'yCGr_G

Cuts at:      0     953    1335 
   Size:        953     382
 
BanII G_rGCy'C

Cuts at:      0     633    1335 
   Size:        633     702
 
BclI T'GATC_A

Cuts at:      0     609    1335 
   Size:        609     726
 
BfrBI ATG'CAT

Cuts at:      0    1131    1335 
   Size:       1131     204
 
BglII A'GATC_T

Cuts at:      0     458    1211    1335 
   Size:        458     753     124

  Fragments arranged by size: 

                 753     458     124
 
BlpI GC'TnA_GC

Cuts at:      0     868    1335 
   Size:        868     467
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     453    1130    1335 
   Size:        453     677     205

  Fragments arranged by size: 

                 677     453     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     455    1335 
   Size:        455     880
 
BsaAI yAC'GTr

Cuts at:      0    1007    1335 
   Size:       1007     328
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     400     430    1335 
   Size:        400      30     905

  Fragments arranged by size: 

                 905     400      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     928    1335 
   Size:        928     407
 
BsiHKAI G_wGCw'C

Cuts at:      0     633     647    1335 
   Size:        633      14     688

  Fragments arranged by size: 

                 688     633      14
 
BsmI GAATG_Cn'

Cuts at:      0     227    1335 
   Size:        227    1108
 
Bsp1286I G_dGCh'C

Cuts at:      0     633     647    1335 
   Size:        633      14     688

  Fragments arranged by size: 

                 688     633      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     245    1335 
   Size:        245    1090
 
BsrBI CCG'CTC

Cuts at:      0     334    1335 
   Size:        334    1001
 
BsrDI GCAATG_nn'

Cuts at:      0     274    1335 
   Size:        274    1061
 
BsrGI T'GTAC_A

Cuts at:      0     354    1335 
   Size:        354     981
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     651    1335 
   Size:        651     684
 
BstYI r'GATC_y

Cuts at:      0     458     670    1211    1335 
   Size:        458     212     541     124

  Fragments arranged by size: 

                 541     458     212     124
 
BtsI GCAGTG_nn'

Cuts at:      0     991    1335 
   Size:        991     344
 
DraI TTT'AAA

Cuts at:      0     830    1335 
   Size:        830     505
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130    1335 
   Size:        130    1205
 
EaeI y'GGCC_r

Cuts at:      0     755    1335 
   Size:        755     580
 
EcoICRI GAG'CTC

Cuts at:      0     631    1335 
   Size:        631     704
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     461    1057    1335 
   Size:        201     260     596     278

  Fragments arranged by size: 

                 596     278     260     201
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1180    1335 
   Size:       1180     155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     231    1335 
   Size:        231    1104
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1198    1203    1230    1235    1335 
   Size:       1198       5      27       5     100

  Fragments arranged by size: 

                1198     100      27       5       5
 
HincII GTy'rAC

Cuts at:      0     135     279    1190    1335 
   Size:        135     144     911     145

  Fragments arranged by size: 

                 911     145     144     135
 
MfeI C'AATT_G

Cuts at:      0     836    1330    1335 
   Size:        836     494       5

  Fragments arranged by size: 

                 836     494       5
 
MscI TGG'CCA

Cuts at:      0     757    1335 
   Size:        757     578
 
MspA1I CmG'CkG

Cuts at:      0     194     731    1335 
   Size:        194     537     604

  Fragments arranged by size: 

                 604     537     194
 
NsiI A_TGCA'T

Cuts at:      0    1133    1335 
   Size:       1133     202
 
PasI CC'CwG_GG

Cuts at:      0     475    1335 
   Size:        475     860
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1159    1335 
   Size:       1159     176
 
PmlI CAC'GTG

Cuts at:      0    1007    1335 
   Size:       1007     328
 
PpuMI rG'GwC_Cy

Cuts at:      0    1180    1335 
   Size:       1180     155
 
PstI C_TGCA'G

Cuts at:      0     998    1335 
   Size:        998     337
 
PvuII CAG'CTG

Cuts at:      0     194     731    1335 
   Size:        194     537     604

  Fragments arranged by size: 

                 604     537     194
 
SacI G_AGCT'C

Cuts at:      0     633    1335 
   Size:        633     702
 
SfcI C'TryA_G

Cuts at:      0     994    1335 
   Size:        994     341
 
SmaI CCC'GGG

Cuts at:      0     955    1335 
   Size:        955     380
 
SmlI C'TyrA_G

Cuts at:      0     468    1145    1335 
   Size:        468     677     190

  Fragments arranged by size: 

                 677     468     190
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     191     437    1335 
   Size:        191     246     898

  Fragments arranged by size: 

                 898     246     191
 
TatI w'GTAC_w

Cuts at:      0     354    1268    1335 
   Size:        354     914      67

  Fragments arranged by size: 

                 914     354      67
 
XbaI T'CTAG_A

Cuts at:      0     768    1335 
   Size:        768     567
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     426    1191    1335 
   Size:        426     765     144

  Fragments arranged by size: 

                 765     426     144
 
XmaI C'CCGG_G

Cuts at:      0     953    1335 
   Size:        953     382
 

 Enzymes that do cut:

     AclI     AcuI     AvaI    BanII     BclI    BfrBI    BglII     BlpI
    BpuEI     BsaI    BsaAI    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrGI   BstAPI    BstYI     BtsI     DraI
     DrdI     EaeI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I   HincII
     MfeI     MscI   MspA1I     NsiI     PasI    PflMI     PmlI    PpuMI
     PstI    PvuII     SacI     SfcI     SmaI     SmlI    TaqII     TatI
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XhoI     XmnI
     ZraI