Restriction map for
pFN21AB7746
(Linear) (Six-base) MAPSORT of: KIBB7746.seq Check: 2564 from: 1 to: 1461
KIBB7746.orf
With 182 enzymes: SgfI *
March 22, 2012 15:40 ..
AccI GT'mk_AC
Cuts at: 0 131 1461
Size: 131 1330
AclI AA'CG_TT
Cuts at: 0 358 1461
Size: 358 1103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1250 1315 1461
Size: 674 576 65 146
Fragments arranged by size:
674 576 146 65
AflII C'TTAA_G
Cuts at: 0 400 1306 1461
Size: 400 906 155
Fragments arranged by size:
906 400 155
AflIII A'CryG_T
Cuts at: 0 1076 1461
Size: 1076 385
AhdI GACnn_n'nnGTC
Cuts at: 0 621 1461
Size: 621 840
AleI CACnn'nnGTG
Cuts at: 0 1102 1461
Size: 1102 359
AlwNI CAG_nnn'CTG
Cuts at: 0 825 1461
Size: 825 636
ApoI r'AATT_y
Cuts at: 0 1260 1461
Size: 1260 201
AseI AT'TA_AT
Cuts at: 0 881 1461
Size: 881 580
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 591 624 1461
Size: 591 33 837
Fragments arranged by size:
837 591 33
BanI G'GyrC_C
Cuts at: 0 1096 1461
Size: 1096 365
BanII G_rGCy'C
Cuts at: 0 796 1461
Size: 796 665
BbvCI CC'TCA_GC
Cuts at: 0 16 1461
Size: 16 1445
BciVI GTATCCnnnnn_n'
Cuts at: 0 592 1461
Size: 592 869
BclI T'GATC_A
Cuts at: 0 1106 1461
Size: 1106 355
BglI GCCn_nnn'nGGC
Cuts at: 0 33 1432 1461
Size: 33 1399 29
Fragments arranged by size:
1399 33 29
Bme1580I G_kGCm'C
Cuts at: 0 1018 1099 1461
Size: 1018 81 362
Fragments arranged by size:
1018 362 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 1102 1461
Size: 1102 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 560 1461
Size: 184 376 901
Fragments arranged by size:
901 376 184
Bpu10I CC'TnA_GC
Cuts at: 0 16 797 1461
Size: 16 781 664
Fragments arranged by size:
781 664 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 1150 1461
Size: 145 1005 311
Fragments arranged by size:
1005 311 145
BsaBI GATnn'nnATC
Cuts at: 0 680 1461
Size: 680 781
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 702 732 1461
Size: 702 30 729
Fragments arranged by size:
729 702 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 710 1461
Size: 710 751
BseYI C'CCAG_C
Cuts at: 0 920 1461
Size: 920 541
BsiHKAI G_wGCw'C
Cuts at: 0 1070 1461
Size: 1070 391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1437 1461
Size: 1437 24
Bsp1286I G_dGCh'C
Cuts at: 0 796 1018 1070 1099 1461
Size: 796 222 52 29 362
Fragments arranged by size:
796 362 222 52 29
BspHI T'CATG_A
Cuts at: 0 701 1461
Size: 701 760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 502 1461
Size: 502 959
BsrDI GCAATG_nn'
Cuts at: 0 241 1461
Size: 241 1220
BsrFI r'CCGG_y
Cuts at: 0 32 154 1461
Size: 32 122 1307
Fragments arranged by size:
1307 122 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 493 1461
Size: 493 968
BstXI CCAn_nnnn'nTGG
Cuts at: 0 648 746 1461
Size: 648 98 715
Fragments arranged by size:
715 648 98
BstYI r'GATC_y
Cuts at: 0 1382 1461
Size: 1382 79
BstZ17I GTA'TAC
Cuts at: 0 132 1461
Size: 132 1329
BtgI C'CryG_G
Cuts at: 0 530 890 1100 1461
Size: 530 360 210 361
Fragments arranged by size:
530 361 360 210
BtsI GCAGTG_nn'
Cuts at: 0 1004 1461
Size: 1004 457
EaeI y'GGCC_r
Cuts at: 0 156 533 1461
Size: 156 377 928
Fragments arranged by size:
928 377 156
EarI CTCTTCn'nnn_
Cuts at: 0 956 1121 1330 1373 1435 1461
Size: 956 165 209 43 62 26
Fragments arranged by size:
956 209 165 62 43 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 560 674 1250 1315 1461
Size: 184 376 114 576 65 146
Fragments arranged by size:
576 376 184 146 114 65
EcoO109I rG'GnC_Cy
Cuts at: 0 116 1326 1461
Size: 116 1210 135
Fragments arranged by size:
1210 135 116
EcoRV GAT'ATC
Cuts at: 0 336 447 678 1461
Size: 336 111 231 783
Fragments arranged by size:
783 336 231 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1359 1461
Size: 1359 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 733 765 1461
Size: 607 32 94 32 696
Fragments arranged by size:
696 607 94 32 32
HincII GTy'rAC
Cuts at: 0 362 517 1461
Size: 362 155 944
Fragments arranged by size:
944 362 155
HpaI GTT'AAC
Cuts at: 0 362 1461
Size: 362 1099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1294 1461
Size: 29 1265 167
Fragments arranged by size:
1265 167 29
MscI TGG'CCA
Cuts at: 0 535 1461
Size: 535 926
MslI CAynn'nnrTG
Cuts at: 0 646 895 1102 1461
Size: 646 249 207 359
Fragments arranged by size:
646 359 249 207
MspA1I CmG'CkG
Cuts at: 0 15 39 927 1461
Size: 15 24 888 534
Fragments arranged by size:
888 534 24 15
NaeI GCC'GGC
Cuts at: 0 34 1461
Size: 34 1427
NcoI C'CATG_G
Cuts at: 0 530 890 1100 1461
Size: 530 360 210 361
Fragments arranged by size:
530 361 360 210
NgoMIV G'CCGG_C
Cuts at: 0 32 1461
Size: 32 1429
NspI r_CATG'y
Cuts at: 0 487 1461
Size: 487 974
PacI TTA_AT'TAA
Cuts at: 0 881 1461
Size: 881 580
PasI CC'CwG_GG
Cuts at: 0 627 1461
Size: 627 834
PflMI CCAn_nnn'nTGG
Cuts at: 0 1288 1461
Size: 1288 173
PfoI T'CCnGG_A
Cuts at: 0 200 1418 1461
Size: 200 1218 43
Fragments arranged by size:
1218 200 43
PpuMI rG'GwC_Cy
Cuts at: 0 116 1326 1461
Size: 116 1210 135
Fragments arranged by size:
1210 135 116
PstI C_TGCA'G
Cuts at: 0 830 1461
Size: 830 631
PvuII CAG'CTG
Cuts at: 0 15 39 1461
Size: 15 24 1422
Fragments arranged by size:
1422 24 15
ScaI AGT'ACT
Cuts at: 0 310 1461
Size: 310 1151
SexAI A'CCwGG_T
Cuts at: 0 1245 1461
Size: 1245 216
SfcI C'TryA_G
Cuts at: 0 826 1461
Size: 826 635
SmlI C'TyrA_G
Cuts at: 0 400 1306 1461
Size: 400 906 155
Fragments arranged by size:
906 400 155
SspI AAT'ATT
Cuts at: 0 262 386 462 1192 1461
Size: 262 124 76 730 269
Fragments arranged by size:
730 269 262 124 76
StyI C'CwwG_G
Cuts at: 0 530 890 1100 1461
Size: 530 360 210 361
Fragments arranged by size:
530 361 360 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 848 991 1461
Size: 848 143 470
Fragments arranged by size:
848 470 143
TatI w'GTAC_w
Cuts at: 0 308 1461
Size: 308 1153
XmnI GAAnn'nnTTC
Cuts at: 0 416 584 1461
Size: 416 168 877
Fragments arranged by size:
877 416 168
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AleI AlwNI
ApoI AseI BaeI BanI BanII BbvCI BciVI BclI
BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BstAPI BstXI BstYI BstZ17I BtgI BtsI EaeI
EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HpaI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PacI PasI PflMI PfoI PpuMI PstI PvuII ScaI
SexAI SfcI SmlI SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AlfI AloI ApaI
ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BbsI
BcgI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BsrBI BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI