Restriction map for
pFN21AB7739
(Linear) (Six-base) MAPSORT of: KIBB7739.seq Check: 9394 from: 1 to: 1308
KIBB7739.orf
With 182 enzymes: SgfI *
March 22, 2012 15:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1044 1308
Size: 1044 264
AccI GT'mk_AC
Cuts at: 0 860 1308
Size: 860 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 379 568 983 1308
Size: 44 335 189 415 325
Fragments arranged by size:
415 335 325 189 44
AflIII A'CryG_T
Cuts at: 0 1104 1308
Size: 1104 204
AseI AT'TA_AT
Cuts at: 0 53 1308
Size: 53 1255
BanI G'GyrC_C
Cuts at: 0 161 1308
Size: 161 1147
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 1163 1308
Size: 32 1131 145
Fragments arranged by size:
1131 145 32
BbvCI CC'TCA_GC
Cuts at: 0 488 680 1308
Size: 488 192 628
Fragments arranged by size:
628 488 192
BclI T'GATC_A
Cuts at: 0 456 867 1308
Size: 456 411 441
Fragments arranged by size:
456 441 411
BglII A'GATC_T
Cuts at: 0 278 744 1308
Size: 278 466 564
Fragments arranged by size:
564 466 278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 1308
Size: 429 879
Bpu10I CC'TnA_GC
Cuts at: 0 166 488 680 1308
Size: 166 322 192 628
Fragments arranged by size:
628 322 192 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1136 1308
Size: 199 937 172
Fragments arranged by size:
937 199 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 282 556 575 824 1308
Size: 282 274 19 249 484
Fragments arranged by size:
484 282 274 249 19
BsaHI Gr'CG_yC
Cuts at: 0 632 1308
Size: 632 676
BsaWI w'CCGG_w
Cuts at: 0 853 1308
Size: 853 455
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 1308
Size: 187 30 1091
Fragments arranged by size:
1091 187 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 668 1308
Size: 280 388 640
Fragments arranged by size:
640 388 280
BseYI C'CCAG_C
Cuts at: 0 44 173 1308
Size: 44 129 1135
Fragments arranged by size:
1135 129 44
BsiEI CG_ry'CG
Cuts at: 0 249 1308
Size: 249 1059
BsiHKAI G_wGCw'C
Cuts at: 0 559 1308
Size: 559 749
BsmI GAATG_Cn'
Cuts at: 0 156 1308
Size: 156 1152
BsmBI CGTCTCn'nnnn_
Cuts at: 0 540 1308
Size: 540 768
Bsp1286I G_dGCh'C
Cuts at: 0 559 1308
Size: 559 749
BspEI T'CCGG_A
Cuts at: 0 853 1308
Size: 853 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 842 1044 1308
Size: 300 542 202 264
Fragments arranged by size:
542 300 264 202
BstEII G'GTnAC_C
Cuts at: 0 396 1308
Size: 396 912
BstXI CCAn_nnnn'nTGG
Cuts at: 0 643 1308
Size: 643 665
BstYI r'GATC_y
Cuts at: 0 278 744 1308
Size: 278 466 564
Fragments arranged by size:
564 466 278
BtsI GCAGTG_nn'
Cuts at: 0 1204 1266 1308
Size: 1204 62 42
Fragments arranged by size:
1204 62 42
EaeI y'GGCC_r
Cuts at: 0 106 246 1066 1308
Size: 106 140 820 242
Fragments arranged by size:
820 242 140 106
EagI C'GGCC_G
Cuts at: 0 246 1308
Size: 246 1062
EarI CTCTTCn'nnn_
Cuts at: 0 492 578 1002 1088 1174 1308
Size: 492 86 424 86 86 134
Fragments arranged by size:
492 424 134 86 86 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 379 429 568 983 1308
Size: 44 335 50 139 415 325
Fragments arranged by size:
415 335 325 139 50 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 374 1308
Size: 374 934
FspI TGC'GCA
Cuts at: 0 155 184 1308
Size: 155 29 1124
Fragments arranged by size:
1124 155 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 281 313 553 555 585 587 711 743
Size: 281 32 240 2 30 2 124 32
Cuts at: 743 903 935 1308
Size: 160 32 373
Fragments arranged by size:
373 281 240 160 124 32 32 32
30 2 2
MfeI C'AATT_G
Cuts at: 0 637 1308
Size: 637 671
MscI TGG'CCA
Cuts at: 0 108 1068 1308
Size: 108 960 240
Fragments arranged by size:
960 240 108
MspA1I CmG'CkG
Cuts at: 0 159 173 327 965 1078 1308
Size: 159 14 154 638 113 230
Fragments arranged by size:
638 230 159 154 113 14
NotI GC'GGCC_GC
Cuts at: 0 246 1308
Size: 246 1062
NspI r_CATG'y
Cuts at: 0 1108 1308
Size: 1108 200
PciI A'CATG_T
Cuts at: 0 1104 1308
Size: 1104 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 407 1308
Size: 407 901
PfoI T'CCnGG_A
Cuts at: 0 98 623 1308
Size: 98 525 685
Fragments arranged by size:
685 525 98
PstI C_TGCA'G
Cuts at: 0 1041 1308
Size: 1041 267
PvuII CAG'CTG
Cuts at: 0 173 965 1308
Size: 173 792 343
Fragments arranged by size:
792 343 173
SapI GCTCTTCn'nnn_
Cuts at: 0 492 1308
Size: 492 816
SexAI A'CCwGG_T
Cuts at: 0 565 1308
Size: 565 743
SfcI C'TryA_G
Cuts at: 0 132 819 1037 1308
Size: 132 687 218 271
Fragments arranged by size:
687 271 218 132
SmlI C'TyrA_G
Cuts at: 0 214 1151 1308
Size: 214 937 157
Fragments arranged by size:
937 214 157
SspI AAT'ATT
Cuts at: 0 1201 1308
Size: 1201 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 220 1308
Size: 220 1088
TatI w'GTAC_w
Cuts at: 0 1101 1308
Size: 1101 207
Tth111I GACn'n_nGTC
Cuts at: 0 567 1308
Size: 567 741
Enzymes that do cut:
AarI AccI AcuI AflIII AseI BanI BbsI BbvCI
BclI BglII BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BstEII BstXI BstYI BtsI EaeI EagI
EarI Eco57MI EcoNI FspI Hin4I MfeI MscI MspA1I
NotI NspI PciI PflMI PfoI PstI PvuII SapI
SexAI SfcI SmlI SspI TaqII TatI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaAI BsaBI BsgI BsiWI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI StyI SwaI TstI XbaI
XcmI XhoI XmaI XmnI ZraI