Restriction map for
pFN21AB7735
(Linear) (Six-base) MAPSORT of: KIBB7735.seq Check: 720 from: 1 to: 1308
KIBB7735.orf
With 182 enzymes: SgfI *
March 22, 2012 15:40 ..
AccI GT'mk_AC
Cuts at: 0 1052 1308
Size: 1052 256
Acc65I G'GTAC_C
Cuts at: 0 1277 1308
Size: 1277 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1250 1308
Size: 654 596 58
Fragments arranged by size:
654 596 58
AflIII A'CryG_T
Cuts at: 0 722 1308
Size: 722 586
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 1308
Size: 1234 32 42
Fragments arranged by size:
1234 42 32
AlwNI CAG_nnn'CTG
Cuts at: 0 330 1308
Size: 330 978
ApaI G_GGCC'C
Cuts at: 0 1093 1308
Size: 1093 215
AvaI C'yCGr_G
Cuts at: 0 562 1308
Size: 562 746
BamHI G'GATC_C
Cuts at: 0 469 1308
Size: 469 839
BanI G'GyrC_C
Cuts at: 0 452 1277 1308
Size: 452 825 31
Fragments arranged by size:
825 452 31
BanII G_rGCy'C
Cuts at: 0 554 1093 1308
Size: 554 539 215
Fragments arranged by size:
554 539 215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1001 1035 1308
Size: 1001 34 273
Fragments arranged by size:
1001 273 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 330 1308
Size: 330 978
BglII A'GATC_T
Cuts at: 0 144 929 1308
Size: 144 785 379
Fragments arranged by size:
785 379 144
Bme1580I G_kGCm'C
Cuts at: 0 455 518 1093 1308
Size: 455 63 575 215
Fragments arranged by size:
575 455 215 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 431 505 548 1308
Size: 318 113 74 43 760
Fragments arranged by size:
760 318 113 74 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 948 1308
Size: 948 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 265 557 592 766 907 1236 1308
Size: 31 234 292 35 174 141 329 72
Fragments arranged by size:
329 292 234 174 141 72 35 31
Bpu10I CC'TnA_GC
Cuts at: 0 709 1185 1308
Size: 709 476 123
Fragments arranged by size:
709 476 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1308
Size: 718 590
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 859 1088 1308
Size: 409 450 229 220
Fragments arranged by size:
450 409 229 220
BsaHI Gr'CG_yC
Cuts at: 0 996 1308
Size: 996 312
BsaWI w'CCGG_w
Cuts at: 0 217 1308
Size: 217 1091
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 880 910 1234 1264 1308
Size: 880 30 324 30 44
Fragments arranged by size:
880 324 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 397 400 580 940 1308
Size: 367 30 3 180 360 368
Fragments arranged by size:
368 367 360 180 30 3
BseYI C'CCAG_C
Cuts at: 0 344 500 529 612 802 814 916 1308
Size: 344 156 29 83 190 12 102 392
Fragments arranged by size:
392 344 190 156 102 83 29 12
BsiHKAI G_wGCw'C
Cuts at: 0 799 1308
Size: 799 509
Bsp1286I G_dGCh'C
Cuts at: 0 455 518 554 799 1093 1308
Size: 455 63 36 245 294 215
Fragments arranged by size:
455 294 245 215 63 36
BspEI T'CCGG_A
Cuts at: 0 217 1308
Size: 217 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 213 1308
Size: 213 1095
BsrBI CCG'CTC
Cuts at: 0 279 1308
Size: 279 1029
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 363 1308
Size: 291 72 945
Fragments arranged by size:
945 291 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 232 448 1308
Size: 232 216 860
Fragments arranged by size:
860 232 216
BstYI r'GATC_y
Cuts at: 0 144 469 929 1270 1308
Size: 144 325 460 341 38
Fragments arranged by size:
460 341 325 144 38
Bsu36I CC'TnA_GG
Cuts at: 0 1077 1308
Size: 1077 231
BtgI C'CryG_G
Cuts at: 0 59 956 1308
Size: 59 897 352
Fragments arranged by size:
897 352 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 658 1308
Size: 658 650
BtsI GCAGTG_nn'
Cuts at: 0 1208 1244 1308
Size: 1208 36 64
Fragments arranged by size:
1208 64 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 21 56 1308
Size: 21 35 1252
Fragments arranged by size:
1252 35 21
EaeI y'GGCC_r
Cuts at: 0 181 303 1028 1308
Size: 181 122 725 280
Fragments arranged by size:
725 280 181 122
EarI CTCTTCn'nnn_
Cuts at: 0 835 886 1269 1308
Size: 835 51 383 39
Fragments arranged by size:
835 383 51 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 1236 1308
Size: 865 371 72
Fragments arranged by size:
865 371 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 265 557 592 654 766 907 1236
Size: 31 234 292 35 62 112 141 329
Cuts at: 1236 1250 1308
Size: 14 58
Fragments arranged by size:
329 292 234 141 112 62 58 35
31 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1184 1308
Size: 1184 124
EcoO109I rG'GnC_Cy
Cuts at: 0 202 699 1090 1308
Size: 202 497 391 218
Fragments arranged by size:
497 391 218 202
EcoRV GAT'ATC
Cuts at: 0 30 1308
Size: 30 1278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 633 1308
Size: 633 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 937 969 1308
Size: 754 32 151 32 339
Fragments arranged by size:
754 339 151 32 32
KpnI G_GTAC'C
Cuts at: 0 1281 1308
Size: 1281 27
MscI TGG'CCA
Cuts at: 0 1030 1308
Size: 1030 278
NcoI C'CATG_G
Cuts at: 0 956 1308
Size: 956 352
NspI r_CATG'y
Cuts at: 0 726 1308
Size: 726 582
PasI CC'CwG_GG
Cuts at: 0 457 1153 1308
Size: 457 696 155
Fragments arranged by size:
696 457 155
PciI A'CATG_T
Cuts at: 0 722 1308
Size: 722 586
PflMI CCAn_nnn'nTGG
Cuts at: 0 421 1308
Size: 421 887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 1308
Size: 1234 32 42
Fragments arranged by size:
1234 42 32
PpuMI rG'GwC_Cy
Cuts at: 0 202 1308
Size: 202 1106
PspOMI G'GGCC_C
Cuts at: 0 1089 1308
Size: 1089 219
PspXI vC'TCGA_Gb
Cuts at: 0 562 1308
Size: 562 746
PstI C_TGCA'G
Cuts at: 0 164 368 1308
Size: 164 204 940
Fragments arranged by size:
940 204 164
SfcI C'TryA_G
Cuts at: 0 160 364 1308
Size: 160 204 944
Fragments arranged by size:
944 204 160
SmlI C'TyrA_G
Cuts at: 0 562 733 1308
Size: 562 171 575
Fragments arranged by size:
575 562 171
StuI AGG'CCT
Cuts at: 0 1170 1308
Size: 1170 138
StyI C'CwwG_G
Cuts at: 0 956 1308
Size: 956 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1050 1308
Size: 1050 258
XhoI C'TCGA_G
Cuts at: 0 562 1308
Size: 562 746
Enzymes that do cut:
AccI Acc65I AcuI AflIII AloI AlwNI ApaI AvaI
BamHI BanI BanII BcgI BciVI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrBI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I
KpnI MscI NcoI NspI PasI PciI PflMI PpiI
PpuMI PspOMI PspXI PstI SfcI SmlI StuI StyI
TaqII XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BclI BfrBI BglI BlpI BmgBI
BmtI BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI DraI DraIII DrdI EagI EcoICRI EcoRI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MmeI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PshAI PsiI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI