Restriction map for
pFN21AB7727
(Linear) (Six-base) MAPSORT of: KIBB7727.seq Check: 3719 from: 1 to: 1293
KIBB7727.orf
With 182 enzymes: SgfI *
March 22, 2012 15:39 ..
AccI GT'mk_AC
Cuts at: 0 624 998 1293
Size: 624 374 295
Fragments arranged by size:
624 374 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1208 1217 1245 1293
Size: 898 310 9 28 48
Fragments arranged by size:
898 310 48 28 9
AfeI AGC'GCT
Cuts at: 0 255 1293
Size: 255 1038
AgeI A'CCGG_T
Cuts at: 0 205 1293
Size: 205 1088
AhdI GACnn_n'nnGTC
Cuts at: 0 1242 1293
Size: 1242 51
AlwNI CAG_nnn'CTG
Cuts at: 0 810 921 1293
Size: 810 111 372
Fragments arranged by size:
810 372 111
AvaI C'yCGr_G
Cuts at: 0 159 1293
Size: 159 1134
BanI G'GyrC_C
Cuts at: 0 163 1293
Size: 163 1130
BanII G_rGCy'C
Cuts at: 0 311 1293
Size: 311 982
BbeI G_GCGC'C
Cuts at: 0 167 1293
Size: 167 1126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 821 855 1293
Size: 821 34 438
Fragments arranged by size:
821 438 34
BglI GCCn_nnn'nGGC
Cuts at: 0 154 1293
Size: 154 1139
Bme1580I G_kGCm'C
Cuts at: 0 496 1293
Size: 496 797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 832 1002 1293
Size: 325 507 170 291
Fragments arranged by size:
507 325 291 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 881 1293
Size: 352 529 412
Fragments arranged by size:
529 412 352
BsaHI Gr'CG_yC
Cuts at: 0 164 1293
Size: 164 1129
BsaWI w'CCGG_w
Cuts at: 0 205 1097 1293
Size: 205 892 196
Fragments arranged by size:
892 205 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 348 378 1293
Size: 348 30 915
Fragments arranged by size:
915 348 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 1293
Size: 607 686
BseYI C'CCAG_C
Cuts at: 0 263 875 1293
Size: 263 612 418
Fragments arranged by size:
612 418 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1134 1293
Size: 337 797 159
Fragments arranged by size:
797 337 159
BsiHKAI G_wGCw'C
Cuts at: 0 231 1293
Size: 231 1062
BsmI GAATG_Cn'
Cuts at: 0 343 1293
Size: 343 950
Bsp1286I G_dGCh'C
Cuts at: 0 231 311 496 1293
Size: 231 80 185 797
Fragments arranged by size:
797 231 185 80
BspEI T'CCGG_A
Cuts at: 0 1097 1293
Size: 1097 196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 929 1293
Size: 929 364
BsrBI CCG'CTC
Cuts at: 0 882 1293
Size: 882 411
BsrDI GCAATG_nn'
Cuts at: 0 445 797 1293
Size: 445 352 496
Fragments arranged by size:
496 445 352
BsrFI r'CCGG_y
Cuts at: 0 102 205 1293
Size: 102 103 1088
Fragments arranged by size:
1088 103 102
BsrGI T'GTAC_A
Cuts at: 0 486 1293
Size: 486 807
BstEII G'GTnAC_C
Cuts at: 0 662 1211 1293
Size: 662 549 82
Fragments arranged by size:
662 549 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 463 792 1293
Size: 463 329 501
Fragments arranged by size:
501 463 329
BstYI r'GATC_y
Cuts at: 0 390 1094 1293
Size: 390 704 199
Fragments arranged by size:
704 390 199
Bsu36I CC'TnA_GG
Cuts at: 0 1104 1293
Size: 1104 189
BtgI C'CryG_G
Cuts at: 0 13 197 1293
Size: 13 184 1096
Fragments arranged by size:
1096 184 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 828 1293
Size: 828 465
EaeI y'GGCC_r
Cuts at: 0 794 1293
Size: 794 499
EarI CTCTTCn'nnn_
Cuts at: 0 986 1293
Size: 986 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 832 898 1002 1208 1217 1245 1293
Size: 325 507 66 104 206 9 28 48
Fragments arranged by size:
507 325 206 104 66 48 28 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 1293
Size: 580 713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 346 1293
Size: 346 947
HaeII r_GCGC'y
Cuts at: 0 167 257 1040 1293
Size: 167 90 783 253
Fragments arranged by size:
783 253 167 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1228 1260 1293
Size: 1051 32 145 32 33
Fragments arranged by size:
1051 145 33 32 32
HincII GTy'rAC
Cuts at: 0 744 1293
Size: 744 549
KasI G'GCGC_C
Cuts at: 0 163 1293
Size: 163 1130
MscI TGG'CCA
Cuts at: 0 796 1293
Size: 796 497
MslI CAynn'nnrTG
Cuts at: 0 510 689 1293
Size: 510 179 604
Fragments arranged by size:
604 510 179
MspA1I CmG'CkG
Cuts at: 0 15 1293
Size: 15 1278
NaeI GCC'GGC
Cuts at: 0 104 1293
Size: 104 1189
NarI GG'CG_CC
Cuts at: 0 164 1293
Size: 164 1129
NcoI C'CATG_G
Cuts at: 0 197 1293
Size: 197 1096
NgoMIV G'CCGG_C
Cuts at: 0 102 1293
Size: 102 1191
NspI r_CATG'y
Cuts at: 0 1153 1293
Size: 1153 140
PasI CC'CwG_GG
Cuts at: 0 76 1293
Size: 76 1217
PflMI CCAn_nnn'nTGG
Cuts at: 0 585 659 1293
Size: 585 74 634
Fragments arranged by size:
634 585 74
PfoI T'CCnGG_A
Cuts at: 0 536 1293
Size: 536 757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 1293
Size: 162 32 1099
Fragments arranged by size:
1099 162 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 1293
Size: 1024 32 237
Fragments arranged by size:
1024 237 32
PstI C_TGCA'G
Cuts at: 0 41 1293
Size: 41 1252
SacII CC_GC'GG
Cuts at: 0 16 1293
Size: 16 1277
SexAI A'CCwGG_T
Cuts at: 0 788 1293
Size: 788 505
SfcI C'TryA_G
Cuts at: 0 37 222 1293
Size: 37 185 1071
Fragments arranged by size:
1071 185 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 154 1293
Size: 154 1139
SfoI GGC'GCC
Cuts at: 0 165 1293
Size: 165 1128
SmlI C'TyrA_G
Cuts at: 0 331 896 1293
Size: 331 565 397
Fragments arranged by size:
565 397 331
SspI AAT'ATT
Cuts at: 0 756 1293
Size: 756 537
StyI C'CwwG_G
Cuts at: 0 197 936 1293
Size: 197 739 357
Fragments arranged by size:
739 357 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 284 434 949 1293
Size: 284 150 515 344
Fragments arranged by size:
515 344 284 150
TatI w'GTAC_w
Cuts at: 0 486 1293
Size: 486 807
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 348 380 997 1029 1293
Size: 348 32 617 32 264
Fragments arranged by size:
617 348 264 32 32
XbaI T'CTAG_A
Cuts at: 0 672 1293
Size: 672 621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1085 1293
Size: 1085 208
Enzymes that do cut:
AccI AcuI AfeI AgeI AhdI AlwNI AvaI BanI
BanII BbeI BcgI BglI Bme1580I BpmI BpuEI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstXI
BstYI Bsu36I BtgI BtgZI EaeI EarI Eco57MI EcoNI
FalI HaeII Hin4I HincII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpiI PsrI PstI SacII SexAI SfcI SfiI SfoI
SmlI SspI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsiEI BsiWI BsmBI BspHI BssHII BssSI BstAPI
BstBI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XhoI XmaI XmnI ZraI