Restriction map for
pFN21AB7696
(Linear) (Six-base) MAPSORT of: KIBB7696.seq Check: 5396 from: 1 to: 1242
KIBB7696.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 1242
Size: 645 597
AflIII A'CryG_T
Cuts at: 0 338 1242
Size: 338 904
ApaI G_GGCC'C
Cuts at: 0 142 681 1242
Size: 142 539 561
Fragments arranged by size:
561 539 142
AvaI C'yCGr_G
Cuts at: 0 195 1242
Size: 195 1047
BamHI G'GATC_C
Cuts at: 0 507 1242
Size: 507 735
BanI G'GyrC_C
Cuts at: 0 42 75 103 1242
Size: 42 33 28 1139
Fragments arranged by size:
1139 42 33 28
BanII G_rGCy'C
Cuts at: 0 142 569 681 1242
Size: 142 427 112 561
Fragments arranged by size:
561 427 142 112
BbeI G_GCGC'C
Cuts at: 0 79 107 1242
Size: 79 28 1135
Fragments arranged by size:
1135 79 28
BfrBI ATG'CAT
Cuts at: 0 599 1242
Size: 599 643
BglI GCCn_nnn'nGGC
Cuts at: 0 73 137 163 182 191 1242
Size: 73 64 26 19 9 1051
Fragments arranged by size:
1051 73 64 26 19 9
Bme1580I G_kGCm'C
Cuts at: 0 47 89 142 681 1242
Size: 47 42 53 539 561
Fragments arranged by size:
561 539 53 47 42
BmgBI CAC'GTC
Cuts at: 0 578 795 1242
Size: 578 217 447
Fragments arranged by size:
578 447 217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1242
Size: 765 477
Bpu10I CC'TnA_GC
Cuts at: 0 331 1242
Size: 331 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 928 1242
Size: 889 39 314
Fragments arranged by size:
889 314 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 985 1242
Size: 985 257
BsaAI yAC'GTr
Cuts at: 0 20 284 341 1242
Size: 20 264 57 901
Fragments arranged by size:
901 264 57 20
BsaHI Gr'CG_yC
Cuts at: 0 76 104 124 1242
Size: 76 28 20 1118
Fragments arranged by size:
1118 76 28 20
BsaWI w'CCGG_w
Cuts at: 0 353 535 1242
Size: 353 182 707
Fragments arranged by size:
707 353 182
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1110 1113 1242
Size: 1110 3 129
Fragments arranged by size:
1110 129 3
BseYI C'CCAG_C
Cuts at: 0 141 470 1242
Size: 141 329 772
Fragments arranged by size:
772 329 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 898 1242
Size: 466 432 344
Fragments arranged by size:
466 432 344
BsiEI CG_ry'CG
Cuts at: 0 170 849 1242
Size: 170 679 393
Fragments arranged by size:
679 393 170
BsiHKAI G_wGCw'C
Cuts at: 0 781 1242
Size: 781 461
BsmI GAATG_Cn'
Cuts at: 0 771 1242
Size: 771 471
BsmBI CGTCTCn'nnnn_
Cuts at: 0 426 1122 1242
Size: 426 696 120
Fragments arranged by size:
696 426 120
Bsp1286I G_dGCh'C
Cuts at: 0 47 89 142 569 681 781 1242
Size: 47 42 53 427 112 100 461
Fragments arranged by size:
461 427 112 100 53 47 42
BspEI T'CCGG_A
Cuts at: 0 535 1242
Size: 535 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 713 1242
Size: 713 529
BsrBI CCG'CTC
Cuts at: 0 260 804 1242
Size: 260 544 438
Fragments arranged by size:
544 438 260
BsrDI GCAATG_nn'
Cuts at: 0 11 1242
Size: 11 1231
BsrFI r'CCGG_y
Cuts at: 0 169 817 1242
Size: 169 648 425
Fragments arranged by size:
648 425 169
BssHII G'CGCG_C
Cuts at: 0 558 1242
Size: 558 684
BstAPI GCAn_nnn'nTGC
Cuts at: 0 389 596 1242
Size: 389 207 646
Fragments arranged by size:
646 389 207
BstYI r'GATC_y
Cuts at: 0 507 878 1242
Size: 507 371 364
Fragments arranged by size:
507 371 364
BtgI C'CryG_G
Cuts at: 0 480 1242
Size: 480 762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 364 1242
Size: 364 878
BtsI GCAGTG_nn'
Cuts at: 0 781 1242
Size: 781 461
DrdI GACnn_nn'nnGTC
Cuts at: 0 853 1242
Size: 853 389
EaeI y'GGCC_r
Cuts at: 0 129 167 174 273 644 809 1222 1242
Size: 129 38 7 99 371 165 413 20
Fragments arranged by size:
413 371 165 129 99 38 20 7
EagI C'GGCC_G
Cuts at: 0 167 1242
Size: 167 1075
EarI CTCTTCn'nnn_
Cuts at: 0 664 1242
Size: 664 578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 831 1242
Size: 831 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 765 1242
Size: 645 120 477
Fragments arranged by size:
645 477 120
EcoO109I rG'GnC_Cy
Cuts at: 0 138 1242
Size: 138 1104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 1242
Size: 321 921
HaeII r_GCGC'y
Cuts at: 0 79 107 668 913 1242
Size: 79 28 561 245 329
Fragments arranged by size:
561 329 245 79 28
HincII GTy'rAC
Cuts at: 0 921 1242
Size: 921 321
HindIII A'AGCT_T
Cuts at: 0 1005 1084 1242
Size: 1005 79 158
Fragments arranged by size:
1005 158 79
KasI G'GCGC_C
Cuts at: 0 75 103 1242
Size: 75 28 1139
Fragments arranged by size:
1139 75 28
MfeI C'AATT_G
Cuts at: 0 1138 1242
Size: 1138 104
MscI TGG'CCA
Cuts at: 0 176 1242
Size: 176 1066
MspA1I CmG'CkG
Cuts at: 0 938 1242
Size: 938 304
NaeI GCC'GGC
Cuts at: 0 819 1242
Size: 819 423
NarI GG'CG_CC
Cuts at: 0 76 104 1242
Size: 76 28 1138
Fragments arranged by size:
1138 76 28
NgoMIV G'CCGG_C
Cuts at: 0 817 1242
Size: 817 425
NsiI A_TGCA'T
Cuts at: 0 601 1242
Size: 601 641
NspI r_CATG'y
Cuts at: 0 603 829 1242
Size: 603 226 413
Fragments arranged by size:
603 413 226
PfoI T'CCnGG_A
Cuts at: 0 1187 1242
Size: 1187 55
PmlI CAC'GTG
Cuts at: 0 20 284 341 1242
Size: 20 264 57 901
Fragments arranged by size:
901 264 57 20
PspOMI G'GGCC_C
Cuts at: 0 138 677 1242
Size: 138 539 565
Fragments arranged by size:
565 539 138
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 319 351 1242
Size: 319 32 891
Fragments arranged by size:
891 319 32
PstI C_TGCA'G
Cuts at: 0 710 1242
Size: 710 532
PvuII CAG'CTG
Cuts at: 0 938 1242
Size: 938 304
SapI GCTCTTCn'nnn_
Cuts at: 0 664 1242
Size: 664 578
ScaI AGT'ACT
Cuts at: 0 397 1242
Size: 397 845
SexAI A'CCwGG_T
Cuts at: 0 719 1242
Size: 719 523
SfcI C'TryA_G
Cuts at: 0 706 1242
Size: 706 536
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 137 182 191 1242
Size: 137 45 9 1051
Fragments arranged by size:
1051 137 45 9
SfoI GGC'GCC
Cuts at: 0 77 105 1242
Size: 77 28 1137
Fragments arranged by size:
1137 77 28
SmaI CCC'GGG
Cuts at: 0 197 1242
Size: 197 1045
SmlI C'TyrA_G
Cuts at: 0 904 943 1242
Size: 904 39 299
Fragments arranged by size:
904 299 39
SphI G_CATG'C
Cuts at: 0 603 829 1242
Size: 603 226 413
Fragments arranged by size:
603 413 226
StuI AGG'CCT
Cuts at: 0 67 1242
Size: 67 1175
StyI C'CwwG_G
Cuts at: 0 132 1242
Size: 132 1110
TatI w'GTAC_w
Cuts at: 0 395 932 1242
Size: 395 537 310
Fragments arranged by size:
537 395 310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 615 1242
Size: 184 431 627
Fragments arranged by size:
627 431 184
XmaI C'CCGG_G
Cuts at: 0 195 1242
Size: 195 1047
Enzymes that do cut:
AcuI AflIII ApaI AvaI BamHI BanI BanII BbeI
BfrBI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstYI BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I FalI HaeII HincII
HindIII KasI MfeI MscI MspA1I NaeI NarI NgoMIV
NsiI NspI PfoI PmlI PspOMI PsrI PstI PvuII
SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI BmrI BmtI BplI BsaBI BsaXI
BsiWI BspHI BsrGI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HpaI KpnI MluI
MmeI MslI NcoI NdeI NheI NotI NruI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI