Restriction map for
pFN21AB7693
(Linear) (Six-base) MAPSORT of: KIBB7693.seq Check: 1040 from: 1 to: 1050
KIBB7693.orf
With 182 enzymes: SgfI *
March 22, 2012 15:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 772 907 1050
Size: 171 601 135 143
Fragments arranged by size:
601 171 143 135
AlwNI CAG_nnn'CTG
Cuts at: 0 454 1050
Size: 454 596
ApaI G_GGCC'C
Cuts at: 0 960 1050
Size: 960 90
AscI GG'CGCG_CC
Cuts at: 0 329 1050
Size: 329 721
AvaI C'yCGr_G
Cuts at: 0 309 366 1050
Size: 309 57 684
Fragments arranged by size:
684 309 57
BanI G'GyrC_C
Cuts at: 0 18 1035 1050
Size: 18 1017 15
Fragments arranged by size:
1017 18 15
BanII G_rGCy'C
Cuts at: 0 767 960 1002 1050
Size: 767 193 42 48
Fragments arranged by size:
767 193 48 42
BbeI G_GCGC'C
Cuts at: 0 22 1039 1050
Size: 22 1017 11
Fragments arranged by size:
1017 22 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 643 1050
Size: 643 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 69 103 1050
Size: 69 34 947
Fragments arranged by size:
947 69 34
BclI T'GATC_A
Cuts at: 0 891 1050
Size: 891 159
BglI GCCn_nnn'nGGC
Cuts at: 0 262 274 349 1050
Size: 262 12 75 701
Fragments arranged by size:
701 262 75 12
BlpI GC'TnA_GC
Cuts at: 0 244 1050
Size: 244 806
Bme1580I G_kGCm'C
Cuts at: 0 960 1050
Size: 960 90
BmgBI CAC'GTC
Cuts at: 0 433 1050
Size: 433 617
BmrI ACTGGGnnnn_n'
Cuts at: 0 1040 1050
Size: 1040 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1050
Size: 529 521
Bpu10I CC'TnA_GC
Cuts at: 0 697 1050
Size: 697 353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1050
Size: 400 650
BsaHI Gr'CG_yC
Cuts at: 0 19 1036 1050
Size: 19 1017 14
Fragments arranged by size:
1017 19 14
BsaWI w'CCGG_w
Cuts at: 0 658 1050
Size: 658 392
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 775 1050
Size: 397 378 275
Fragments arranged by size:
397 378 275
BseYI C'CCAG_C
Cuts at: 0 215 959 1050
Size: 215 744 91
Fragments arranged by size:
744 215 91
BsiEI CG_ry'CG
Cuts at: 0 278 815 1050
Size: 278 537 235
Fragments arranged by size:
537 278 235
BsiHKAI G_wGCw'C
Cuts at: 0 308 1050
Size: 308 742
BsmI GAATG_Cn'
Cuts at: 0 483 1050
Size: 483 567
Bsp1286I G_dGCh'C
Cuts at: 0 308 767 960 1002 1050
Size: 308 459 193 42 48
Fragments arranged by size:
459 308 193 48 42
BsrBI CCG'CTC
Cuts at: 0 165 1050
Size: 165 885
BsrFI r'CCGG_y
Cuts at: 0 965 1050
Size: 965 85
BssHII G'CGCG_C
Cuts at: 0 329 1050
Size: 329 721
BtgI C'CryG_G
Cuts at: 0 23 208 257 529 767 809 1050
Size: 23 185 49 272 238 42 241
Fragments arranged by size:
272 241 238 185 49 42 23
DraIII CAC_nnn'GTG
Cuts at: 0 237 1050
Size: 237 813
EaeI y'GGCC_r
Cuts at: 0 275 321 408 812 1050
Size: 275 46 87 404 238
Fragments arranged by size:
404 275 238 87 46
EagI C'GGCC_G
Cuts at: 0 275 812 1050
Size: 275 537 238
Fragments arranged by size:
537 275 238
EarI CTCTTCn'nnn_
Cuts at: 0 748 929 943 1050
Size: 748 181 14 107
Fragments arranged by size:
748 181 107 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 43 345 1050
Size: 43 302 705
Fragments arranged by size:
705 302 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 529 772 907 1050
Size: 171 358 243 135 143
Fragments arranged by size:
358 243 171 143 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 744 1050
Size: 744 306
EcoO109I rG'GnC_Cy
Cuts at: 0 578 906 956 1050
Size: 578 328 50 94
Fragments arranged by size:
578 328 94 50
HaeII r_GCGC'y
Cuts at: 0 22 216 966 990 1039 1050
Size: 22 194 750 24 49 11
Fragments arranged by size:
750 194 49 24 22 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 567 599 1050
Size: 472 32 63 32 451
Fragments arranged by size:
472 451 63 32 32
KasI G'GCGC_C
Cuts at: 0 18 1035 1050
Size: 18 1017 15
Fragments arranged by size:
1017 18 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1050
Size: 453 597
MslI CAynn'nnrTG
Cuts at: 0 569 1050
Size: 569 481
MspA1I CmG'CkG
Cuts at: 0 259 407 811 981 1050
Size: 259 148 404 170 69
Fragments arranged by size:
404 259 170 148 69
NaeI GCC'GGC
Cuts at: 0 967 1050
Size: 967 83
NarI GG'CG_CC
Cuts at: 0 19 1036 1050
Size: 19 1017 14
Fragments arranged by size:
1017 19 14
NcoI C'CATG_G
Cuts at: 0 23 1050
Size: 23 1027
NgoMIV G'CCGG_C
Cuts at: 0 965 1050
Size: 965 85
PasI CC'CwG_GG
Cuts at: 0 250 1050
Size: 250 800
PflMI CCAn_nnn'nTGG
Cuts at: 0 604 1050
Size: 604 446
PpuMI rG'GwC_Cy
Cuts at: 0 906 1050
Size: 906 144
PspOMI G'GGCC_C
Cuts at: 0 956 1050
Size: 956 94
PstI C_TGCA'G
Cuts at: 0 291 974 1050
Size: 291 683 76
Fragments arranged by size:
683 291 76
SacII CC_GC'GG
Cuts at: 0 260 812 1050
Size: 260 552 238
Fragments arranged by size:
552 260 238
SfcI C'TryA_G
Cuts at: 0 287 970 1050
Size: 287 683 80
Fragments arranged by size:
683 287 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 262 1050
Size: 262 788
SfoI GGC'GCC
Cuts at: 0 20 1037 1050
Size: 20 1017 13
Fragments arranged by size:
1017 20 13
SmlI C'TyrA_G
Cuts at: 0 415 1050
Size: 415 635
StyI C'CwwG_G
Cuts at: 0 23 1050
Size: 23 1027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 724 1050
Size: 87 637 326
Fragments arranged by size:
637 326 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 418 1050
Size: 418 632
XmnI GAAnn'nnTTC
Cuts at: 0 920 1050
Size: 920 130
Enzymes that do cut:
AcuI AlwNI ApaI AscI AvaI BanI BanII BbeI
BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BsrBI BsrFI BssHII BtgI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV PasI PflMI PpuMI PspOMI PstI SacII SfcI
SfiI SfoI SmlI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglII
BmtI BplI BsaI BsaAI BsaBI BsaXI BsgI BsiWI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NdeI NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI ZraI