Restriction map for
pFN21AB7681
(Linear) (Six-base) MAPSORT of: KIBB7681.seq Check: 6378 from: 1 to: 1029
KIBB7681.orf
With 182 enzymes: SgfI *
July 3, 2012 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 119 1029
Size: 119 910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1029
Size: 462 567
AhdI GACnn_n'nnGTC
Cuts at: 0 960 1029
Size: 960 69
AlwNI CAG_nnn'CTG
Cuts at: 0 754 1029
Size: 754 275
ApaI G_GGCC'C
Cuts at: 0 82 167 230 268 340 353 1029
Size: 82 85 63 38 72 13 676
Fragments arranged by size:
676 85 82 72 63 38 13
AvaI C'yCGr_G
Cuts at: 0 7 60 223 411 545 584 1029
Size: 7 53 163 188 134 39 445
Fragments arranged by size:
445 188 163 134 53 39 7
BanI G'GyrC_C
Cuts at: 0 858 1029
Size: 858 171
BanII G_rGCy'C
Cuts at: 0 82 167 176 230 268 340 353 763
Size: 82 85 9 54 38 72 13 410
Cuts at: 763 1029
Size: 266
Fragments arranged by size:
410 266 85 82 72 54 38 13
9
BbsI GAAGACnn'nnnn_
Cuts at: 0 292 504 1029
Size: 292 212 525
Fragments arranged by size:
525 292 212
BbvCI CC'TCA_GC
Cuts at: 0 278 719 1029
Size: 278 441 310
Fragments arranged by size:
441 310 278
BfrBI ATG'CAT
Cuts at: 0 809 1029
Size: 809 220
BglI GCCn_nnn'nGGC
Cuts at: 0 335 767 1029
Size: 335 432 262
Fragments arranged by size:
432 335 262
Bme1580I G_kGCm'C
Cuts at: 0 82 167 230 268 340 353 1029
Size: 82 85 63 38 72 13 676
Fragments arranged by size:
676 85 82 72 63 38 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 306 960 1029
Size: 306 654 69
Fragments arranged by size:
654 306 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 514 523 770 785 1029
Size: 190 324 9 247 15 244
Fragments arranged by size:
324 247 244 190 15 9
Bpu10I CC'TnA_GC
Cuts at: 0 278 719 982 1029
Size: 278 441 263 47
Fragments arranged by size:
441 278 263 47
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 511 1029
Size: 295 216 518
Fragments arranged by size:
518 295 216
BseYI C'CCAG_C
Cuts at: 0 311 1029
Size: 311 718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 97 1029
Size: 23 74 932
Fragments arranged by size:
932 74 23
BsiHKAI G_wGCw'C
Cuts at: 0 14 1029
Size: 14 1015
BsmBI CGTCTCn'nnnn_
Cuts at: 0 338 981 1029
Size: 338 643 48
Fragments arranged by size:
643 338 48
Bsp1286I G_dGCh'C
Cuts at: 0 14 82 167 176 230 268 340 353
Size: 14 68 85 9 54 38 72 13
Cuts at: 353 763 1029
Size: 410 266
Fragments arranged by size:
410 266 85 72 68 54 38 14
13 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 221 1029
Size: 119 102 808
Fragments arranged by size:
808 119 102
BsrFI r'CCGG_y
Cuts at: 0 189 1029
Size: 189 840
BssHII G'CGCG_C
Cuts at: 0 17 1029
Size: 17 1012
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 832 1029
Size: 435 397 197
Fragments arranged by size:
435 397 197
BstYI r'GATC_y
Cuts at: 0 647 751 1029
Size: 647 104 278
Fragments arranged by size:
647 278 104
BtgI C'CryG_G
Cuts at: 0 129 136 269 428 831 900 1029
Size: 129 7 133 159 403 69 129
Fragments arranged by size:
403 159 133 129 129 69 7
BtsI GCAGTG_nn'
Cuts at: 0 12 1029
Size: 12 1017
EaeI y'GGCC_r
Cuts at: 0 191 249 386 437 1029
Size: 191 58 137 51 592
Fragments arranged by size:
592 191 137 58 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 639 675 1029
Size: 639 36 354
Fragments arranged by size:
639 354 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 462 514 523 770 785 1029
Size: 190 272 52 9 247 15 244
Fragments arranged by size:
272 247 244 190 52 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 1029
Size: 577 452
EcoO109I rG'GnC_Cy
Cuts at: 0 34 164 219 264 265 336 337 350
Size: 34 130 55 45 1 71 1 13
Cuts at: 350 376 769 1029
Size: 26 393 260
Fragments arranged by size:
393 260 130 71 55 45 34 26
13 1 1
FspI TGC'GCA
Cuts at: 0 565 1029
Size: 565 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 946 978 1029
Size: 337 32 577 32 51
Fragments arranged by size:
577 337 51 32 32
MscI TGG'CCA
Cuts at: 0 388 439 1029
Size: 388 51 590
Fragments arranged by size:
590 388 51
MslI CAynn'nnrTG
Cuts at: 0 691 830 836 856 1029
Size: 691 139 6 20 173
Fragments arranged by size:
691 173 139 20 6
MspA1I CmG'CkG
Cuts at: 0 100 131 138 271 315 492 555 723
Size: 100 31 7 133 44 177 63 168
Cuts at: 723 1029
Size: 306
Fragments arranged by size:
306 177 168 133 100 63 44 31
7
NaeI GCC'GGC
Cuts at: 0 191 1029
Size: 191 838
NcoI C'CATG_G
Cuts at: 0 428 831 900 1029
Size: 428 403 69 129
Fragments arranged by size:
428 403 129 69
NgoMIV G'CCGG_C
Cuts at: 0 189 1029
Size: 189 840
NsiI A_TGCA'T
Cuts at: 0 811 1029
Size: 811 218
NspI r_CATG'y
Cuts at: 0 813 1029
Size: 813 216
PasI CC'CwG_GG
Cuts at: 0 918 1029
Size: 918 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 754 1029
Size: 754 275
PfoI T'CCnGG_A
Cuts at: 0 650 747 1029
Size: 650 97 282
Fragments arranged by size:
650 282 97
PpuMI rG'GwC_Cy
Cuts at: 0 219 1029
Size: 219 810
PspOMI G'GGCC_C
Cuts at: 0 78 163 226 264 336 349 1029
Size: 78 85 63 38 72 13 680
Fragments arranged by size:
680 85 78 72 63 38 13
PstI C_TGCA'G
Cuts at: 0 218 728 1029
Size: 218 510 301
Fragments arranged by size:
510 301 218
PvuII CAG'CTG
Cuts at: 0 315 492 555 723 1029
Size: 315 177 63 168 306
Fragments arranged by size:
315 306 177 168 63
SacII CC_GC'GG
Cuts at: 0 132 139 272 1029
Size: 132 7 133 757
Fragments arranged by size:
757 133 132 7
SbfI CC_TGCA'GG
Cuts at: 0 218 1029
Size: 218 811
SfcI C'TryA_G
Cuts at: 0 214 724 1029
Size: 214 510 305
Fragments arranged by size:
510 305 214
SmaI CCC'GGG
Cuts at: 0 413 547 586 1029
Size: 413 134 39 443
Fragments arranged by size:
443 413 134 39
StyI C'CwwG_G
Cuts at: 0 320 428 831 900 995 1029
Size: 320 108 403 69 95 34
Fragments arranged by size:
403 320 108 95 69 34
XmaI C'CCGG_G
Cuts at: 0 411 545 584 1029
Size: 411 134 39 445
Fragments arranged by size:
445 411 134 39
XmnI GAAnn'nnTTC
Cuts at: 0 511 667 1029
Size: 511 156 362
Fragments arranged by size:
511 362 156
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BfrBI BglI Bme1580I BplI BpmI Bpu10I
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BssHII BstXI BstYI BtgI BtsI EaeI EciI Eco57MI
EcoNI EcoO109I FspI Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacII SbfI SfcI SmaI StyI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI ZraI