Restriction map for
pFN21AB7678
(Linear) (Six-base) MAPSORT of: KIBB7678.seq Check: 5777 from: 1 to: 1218
KIBB7678.orf
With 182 enzymes: SgfI *
July 3, 2012 16:26 ..
AclI AA'CG_TT
Cuts at: 0 179 1218
Size: 179 1039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 557 1218
Size: 337 220 661
Fragments arranged by size:
661 337 220
AflIII A'CryG_T
Cuts at: 0 647 1137 1218
Size: 647 490 81
Fragments arranged by size:
647 490 81
ApaI G_GGCC'C
Cuts at: 0 1182 1218
Size: 1182 36
ApoI r'AATT_y
Cuts at: 0 528 1218
Size: 528 690
AvaI C'yCGr_G
Cuts at: 0 310 859 967 994 1021 1048 1075 1102
Size: 310 549 108 27 27 27 27 27
Cuts at: 1102 1218
Size: 116
Fragments arranged by size:
549 310 116 108 27 27 27 27
27
BamHI G'GATC_C
Cuts at: 0 687 1218
Size: 687 531
BanI G'GyrC_C
Cuts at: 0 711 819 846 873 900 927 954 1035
Size: 711 108 27 27 27 27 27 81
Cuts at: 1035 1062 1089 1116 1218
Size: 27 27 27 102
Fragments arranged by size:
711 108 102 81 27 27 27 27
27 27 27 27
BanII G_rGCy'C
Cuts at: 0 1182 1218
Size: 1182 36
BbeI G_GCGC'C
Cuts at: 0 823 850 877 904 931 958 1039 1066
Size: 823 27 27 27 27 27 81 27
Cuts at: 1066 1093 1120 1218
Size: 27 27 98
Fragments arranged by size:
823 98 81 27 27 27 27 27
27 27 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 1218
Size: 545 673
BbvCI CC'TCA_GC
Cuts at: 0 389 1218
Size: 389 829
BfrBI ATG'CAT
Cuts at: 0 1204 1218
Size: 1204 14
BglI GCCn_nnn'nGGC
Cuts at: 0 762 789 861 888 915 942 969 996
Size: 762 27 72 27 27 27 27 27
Cuts at: 996 1023 1050 1077 1104 1218
Size: 27 27 27 27 114
Fragments arranged by size:
762 114 72 27 27 27 27 27
27 27 27 27 27
Bme1580I G_kGCm'C
Cuts at: 0 1182 1218
Size: 1182 36
Bpu10I CC'TnA_GC
Cuts at: 0 389 1218
Size: 389 829
BsaHI Gr'CG_yC
Cuts at: 0 820 847 874 901 928 955 1036 1063
Size: 820 27 27 27 27 27 81 27
Cuts at: 1063 1090 1117 1218
Size: 27 27 101
Fragments arranged by size:
820 101 81 27 27 27 27 27
27 27 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 145 274 310 346 1218
Size: 134 11 129 36 36 872
Fragments arranged by size:
872 134 129 36 36 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1218
Size: 568 650
BsiEI CG_ry'CG
Cuts at: 0 350 1218
Size: 350 868
Bsp1286I G_dGCh'C
Cuts at: 0 1182 1218
Size: 1182 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 540 1218
Size: 540 678
BsrFI r'CCGG_y
Cuts at: 0 366 1218
Size: 366 852
BsrGI T'GTAC_A
Cuts at: 0 1164 1218
Size: 1164 54
BssSI C'ACGA_G
Cuts at: 0 238 1218
Size: 238 980
BstYI r'GATC_y
Cuts at: 0 687 1218
Size: 687 531
BtgI C'CryG_G
Cuts at: 0 350 770 815 896 923 950 977 1004
Size: 350 420 45 81 27 27 27 27
Cuts at: 1004 1031 1058 1085 1112 1218
Size: 27 27 27 27 106
Fragments arranged by size:
420 350 106 81 45 27 27 27
27 27 27 27 27
BtsI GCAGTG_nn'
Cuts at: 0 614 1218
Size: 614 604
EaeI y'GGCC_r
Cuts at: 0 234 889 916 1218
Size: 234 655 27 302
Fragments arranged by size:
655 302 234 27
EarI CTCTTCn'nnn_
Cuts at: 0 14 564 1218
Size: 14 550 654
Fragments arranged by size:
654 550 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 385 1218
Size: 385 833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 557 1218
Size: 337 220 661
Fragments arranged by size:
661 337 220
EcoO109I rG'GnC_Cy
Cuts at: 0 662 1179 1218
Size: 662 517 39
Fragments arranged by size:
662 517 39
HaeII r_GCGC'y
Cuts at: 0 823 850 877 904 931 958 1039 1066
Size: 823 27 27 27 27 27 81 27
Cuts at: 1066 1093 1120 1218
Size: 27 27 98
Fragments arranged by size:
823 98 81 27 27 27 27 27
27 27 27
KasI G'GCGC_C
Cuts at: 0 819 846 873 900 927 954 1035 1062
Size: 819 27 27 27 27 27 81 27
Cuts at: 1062 1089 1116 1218
Size: 27 27 102
Fragments arranged by size:
819 102 81 27 27 27 27 27
27 27 27
MspA1I CmG'CkG
Cuts at: 0 388 450 1218
Size: 388 62 768
Fragments arranged by size:
768 388 62
NaeI GCC'GGC
Cuts at: 0 368 1218
Size: 368 850
NarI GG'CG_CC
Cuts at: 0 820 847 874 901 928 955 1036 1063
Size: 820 27 27 27 27 27 81 27
Cuts at: 1063 1090 1117 1218
Size: 27 27 101
Fragments arranged by size:
820 101 81 27 27 27 27 27
27 27 27
NcoI C'CATG_G
Cuts at: 0 770 815 896 923 950 977 1004 1031
Size: 770 45 81 27 27 27 27 27
Cuts at: 1031 1058 1085 1112 1218
Size: 27 27 27 106
Fragments arranged by size:
770 106 81 45 27 27 27 27
27 27 27 27
NgoMIV G'CCGG_C
Cuts at: 0 366 1218
Size: 366 852
NsiI A_TGCA'T
Cuts at: 0 1206 1218
Size: 1206 12
NspI r_CATG'y
Cuts at: 0 651 1141 1208 1218
Size: 651 490 67 10
Fragments arranged by size:
651 490 67 10
PciI A'CATG_T
Cuts at: 0 647 1137 1218
Size: 647 490 81
Fragments arranged by size:
647 490 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1218
Size: 642 576
PspOMI G'GGCC_C
Cuts at: 0 1178 1218
Size: 1178 40
PspXI vC'TCGA_Gb
Cuts at: 0 310 1218
Size: 310 908
PstI C_TGCA'G
Cuts at: 0 455 1218
Size: 455 763
PvuII CAG'CTG
Cuts at: 0 450 1218
Size: 450 768
SapI GCTCTTCn'nnn_
Cuts at: 0 564 1218
Size: 564 654
SfcI C'TryA_G
Cuts at: 0 451 1218
Size: 451 767
SfoI GGC'GCC
Cuts at: 0 821 848 875 902 929 956 1037 1064
Size: 821 27 27 27 27 27 81 27
Cuts at: 1064 1091 1118 1218
Size: 27 27 100
Fragments arranged by size:
821 100 81 27 27 27 27 27
27 27 27
SmaI CCC'GGG
Cuts at: 0 861 969 996 1023 1050 1077 1104 1218
Size: 861 108 27 27 27 27 27 114
Fragments arranged by size:
861 114 108 27 27 27 27 27
SmlI C'TyrA_G
Cuts at: 0 310 1218
Size: 310 908
StyI C'CwwG_G
Cuts at: 0 770 815 896 923 950 977 1004 1031
Size: 770 45 81 27 27 27 27 27
Cuts at: 1031 1058 1085 1112 1218
Size: 27 27 27 106
Fragments arranged by size:
770 106 81 45 27 27 27 27
27 27 27 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 646 774 783 801 810 828 1125
Size: 37 609 128 9 18 9 18 297
Cuts at: 1125 1143 1218
Size: 18 75
Fragments arranged by size:
609 297 128 75 37 18 18 18
9 9
TatI w'GTAC_w
Cuts at: 0 1164 1218
Size: 1164 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 758 790 1218
Size: 758 32 428
Fragments arranged by size:
758 428 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 31 768 1218
Size: 31 737 450
Fragments arranged by size:
737 450 31
XhoI C'TCGA_G
Cuts at: 0 310 1218
Size: 310 908
XmaI C'CCGG_G
Cuts at: 0 859 967 994 1021 1048 1075 1102 1218
Size: 859 108 27 27 27 27 27 116
Fragments arranged by size:
859 116 108 27 27 27 27 27
XmnI GAAnn'nnTTC
Cuts at: 0 469 1218
Size: 469 749
Enzymes that do cut:
AclI AcuI AflIII ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BfrBI BglI Bme1580I Bpu10I
BsaHI BseRI BsgI BsiEI Bsp1286I BspMI BsrFI BsrGI
BssSI BstYI BtgI BtsI EaeI EarI EciI Eco57MI
EcoO109I HaeII KasI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PspOMI PspXI PstI PvuII
SapI SfcI SfoI SmaI SmlI StyI TaqII TatI
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BglII BlpI BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NdeI NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI ZraI