Restriction map for
pFN21AB7642
(Linear) (Six-base) MAPSORT of: KIBB7642.seq Check: 6645 from: 1 to: 1161
KIBB7642.orf
With 182 enzymes: SgfI *
March 22, 2012 15:38 ..
Acc65I G'GTAC_C
Cuts at: 0 836 1161
Size: 836 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1161
Size: 528 633
AfeI AGC'GCT
Cuts at: 0 803 1161
Size: 803 358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 547 1161
Size: 547 614
AlwNI CAG_nnn'CTG
Cuts at: 0 342 381 1161
Size: 342 39 780
Fragments arranged by size:
780 342 39
ApaLI G'TGCA_C
Cuts at: 0 470 532 1161
Size: 470 62 629
Fragments arranged by size:
629 470 62
AvaI C'yCGr_G
Cuts at: 0 556 1161
Size: 556 605
AvrII C'CTAG_G
Cuts at: 0 1092 1161
Size: 1092 69
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 473 506 1161
Size: 473 33 655
Fragments arranged by size:
655 473 33
BanI G'GyrC_C
Cuts at: 0 180 836 1068 1144 1161
Size: 180 656 232 76 17
Fragments arranged by size:
656 232 180 76 17
BanII G_rGCy'C
Cuts at: 0 215 388 1161
Size: 215 173 773
Fragments arranged by size:
773 215 173
BbeI G_GCGC'C
Cuts at: 0 184 1161
Size: 184 977
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 1161
Size: 410 751
BciVI GTATCCnnnnn_n'
Cuts at: 0 501 1161
Size: 501 660
BglI GCCn_nnn'nGGC
Cuts at: 0 397 965 1161
Size: 397 568 196
Fragments arranged by size:
568 397 196
Bme1580I G_kGCm'C
Cuts at: 0 474 536 1073 1161
Size: 474 62 537 88
Fragments arranged by size:
537 474 88 62
BmgBI CAC'GTC
Cuts at: 0 416 1161
Size: 416 745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 904 1019 1161
Size: 760 144 115 142
Fragments arranged by size:
760 144 142 115
Bpu10I CC'TnA_GC
Cuts at: 0 769 1161
Size: 769 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 635 1161
Size: 635 526
BsaHI Gr'CG_yC
Cuts at: 0 181 1161
Size: 181 980
BsaWI w'CCGG_w
Cuts at: 0 1025 1161
Size: 1025 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 132 162 1161
Size: 132 30 999
Fragments arranged by size:
999 132 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1022 1161
Size: 1022 139
BseYI C'CCAG_C
Cuts at: 0 245 374 381 963 1049 1161
Size: 245 129 7 582 86 112
Fragments arranged by size:
582 245 129 112 86 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1161
Size: 796 365
BsiEI CG_ry'CG
Cuts at: 0 293 392 1161
Size: 293 99 769
Fragments arranged by size:
769 293 99
BsiHKAI G_wGCw'C
Cuts at: 0 474 536 1082 1161
Size: 474 62 546 79
Fragments arranged by size:
546 474 79 62
Bsp1286I G_dGCh'C
Cuts at: 0 215 388 474 536 1073 1082 1161
Size: 215 173 86 62 537 9 79
Fragments arranged by size:
537 215 173 86 79 62 9
BsrBI CCG'CTC
Cuts at: 0 102 756 1161
Size: 102 654 405
Fragments arranged by size:
654 405 102
BsrFI r'CCGG_y
Cuts at: 0 288 1161
Size: 288 873
BsrGI T'GTAC_A
Cuts at: 0 1010 1161
Size: 1010 151
BssSI C'ACGA_G
Cuts at: 0 535 1161
Size: 535 626
BstAPI GCAn_nnn'nTGC
Cuts at: 0 312 321 342 1161
Size: 312 9 21 819
Fragments arranged by size:
819 312 21 9
BtgI C'CryG_G
Cuts at: 0 119 206 293 621 1086 1161
Size: 119 87 87 328 465 75
Fragments arranged by size:
465 328 119 87 87 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 920 1161
Size: 920 241
BtsI GCAGTG_nn'
Cuts at: 0 536 1161
Size: 536 625
DrdI GACnn_nn'nnGTC
Cuts at: 0 414 823 1161
Size: 414 409 338
Fragments arranged by size:
414 409 338
EaeI y'GGCC_r
Cuts at: 0 290 389 893 1161
Size: 290 99 504 268
Fragments arranged by size:
504 290 268 99
EagI C'GGCC_G
Cuts at: 0 290 389 1161
Size: 290 99 772
Fragments arranged by size:
772 290 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 1161
Size: 85 1076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 760 904 1019 1161
Size: 528 232 144 115 142
Fragments arranged by size:
528 232 144 142 115
EcoO109I rG'GnC_Cy
Cuts at: 0 1096 1161
Size: 1096 65
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 728 1161
Size: 456 272 433
Fragments arranged by size:
456 433 272
HaeII r_GCGC'y
Cuts at: 0 184 805 919 1161
Size: 184 621 114 242
Fragments arranged by size:
621 242 184 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 1161
Size: 234 32 895
Fragments arranged by size:
895 234 32
KasI G'GCGC_C
Cuts at: 0 180 1161
Size: 180 981
KpnI G_GTAC'C
Cuts at: 0 840 1161
Size: 840 321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1161
Size: 743 418
MspA1I CmG'CkG
Cuts at: 0 20 121 295 342 381 874 1003 1053
Size: 20 101 174 47 39 493 129 50
Cuts at: 1053 1059 1161
Size: 6 102
Fragments arranged by size:
493 174 129 102 101 50 47 39
20 6
NaeI GCC'GGC
Cuts at: 0 290 1161
Size: 290 871
NarI GG'CG_CC
Cuts at: 0 181 1161
Size: 181 980
NcoI C'CATG_G
Cuts at: 0 206 1161
Size: 206 955
NgoMIV G'CCGG_C
Cuts at: 0 288 1161
Size: 288 873
NspI r_CATG'y
Cuts at: 0 470 1023 1161
Size: 470 553 138
Fragments arranged by size:
553 470 138
PacI TTA_AT'TAA
Cuts at: 0 431 1161
Size: 431 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 1161
Size: 381 780
PspXI vC'TCGA_Gb
Cuts at: 0 556 1161
Size: 556 605
PstI C_TGCA'G
Cuts at: 0 801 923 1058 1161
Size: 801 122 135 103
Fragments arranged by size:
801 135 122 103
PvuII CAG'CTG
Cuts at: 0 381 1053 1161
Size: 381 672 108
Fragments arranged by size:
672 381 108
SacII CC_GC'GG
Cuts at: 0 122 296 1161
Size: 122 174 865
Fragments arranged by size:
865 174 122
SexAI A'CCwGG_T
Cuts at: 0 832 1161
Size: 832 329
SfcI C'TryA_G
Cuts at: 0 477 797 919 1054 1161
Size: 477 320 122 135 107
Fragments arranged by size:
477 320 135 122 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 397 1161
Size: 397 764
SfoI GGC'GCC
Cuts at: 0 182 1161
Size: 182 979
SmlI C'TyrA_G
Cuts at: 0 556 1161
Size: 556 605
StyI C'CwwG_G
Cuts at: 0 206 1092 1161
Size: 206 886 69
Fragments arranged by size:
886 206 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 835 968 1161
Size: 835 133 193
Fragments arranged by size:
835 193 133
TatI w'GTAC_w
Cuts at: 0 1010 1161
Size: 1010 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 132 164 1161
Size: 132 32 997
Fragments arranged by size:
997 132 32
XhoI C'TCGA_G
Cuts at: 0 556 1161
Size: 556 605
Enzymes that do cut:
Acc65I AcuI AfeI AlfI AlwNI ApaLI AvaI AvrII
BaeI BanI BanII BbeI BbsI BciVI BglI Bme1580I
BmgBI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BsrGI
BssSI BstAPI BtgI BtgZI BtsI DrdI EaeI EagI
EciI Eco57MI EcoO109I FalI HaeII Hin4I KasI KpnI
MmeI MspA1I NaeI NarI NcoI NgoMIV NspI PacI
PflMI PspXI PstI PvuII SacII SexAI SfcI SfiI
SfoI SmlI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI BamHI
BbvCI BcgI BclI BfrBI BglII BlpI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BssHII BstBI BstEII BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MscI MslI NdeI NheI NotI
NruI NsiI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XmaI XmnI ZraI