Restriction map for
pFN21AB7626
(Linear) (Six-base) MAPSORT of: KIBB7626.seq Check: 4213 from: 1 to: 1116
KIBB7626.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1051 1116
Size: 1051 65
Acc65I G'GTAC_C
Cuts at: 0 869 1116
Size: 869 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 944 1116
Size: 814 130 172
Fragments arranged by size:
814 172 130
AgeI A'CCGG_T
Cuts at: 0 837 1116
Size: 837 279
AlwNI CAG_nnn'CTG
Cuts at: 0 460 1116
Size: 460 656
ApaI G_GGCC'C
Cuts at: 0 657 1116
Size: 657 459
BanI G'GyrC_C
Cuts at: 0 138 196 521 551 869 1116
Size: 138 58 325 30 318 247
Fragments arranged by size:
325 318 247 138 58 30
BanII G_rGCy'C
Cuts at: 0 103 657 1116
Size: 103 554 459
Fragments arranged by size:
554 459 103
BbeI G_GCGC'C
Cuts at: 0 142 525 555 1116
Size: 142 383 30 561
Fragments arranged by size:
561 383 142 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 1116
Size: 245 871
BglI GCCn_nnn'nGGC
Cuts at: 0 73 107 1116
Size: 73 34 1009
Fragments arranged by size:
1009 73 34
Bme1580I G_kGCm'C
Cuts at: 0 199 657 1116
Size: 199 458 459
Fragments arranged by size:
459 458 199
BmgBI CAC'GTC
Cuts at: 0 153 535 1116
Size: 153 382 581
Fragments arranged by size:
581 382 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 672 1116
Size: 672 444
BsaAI yAC'GTr
Cuts at: 0 723 1116
Size: 723 393
BsaHI Gr'CG_yC
Cuts at: 0 139 522 552 990 1008 1116
Size: 139 383 30 438 18 108
Fragments arranged by size:
438 383 139 108 30 18
BsaWI w'CCGG_w
Cuts at: 0 837 1116
Size: 837 279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 476 506 1116
Size: 404 30 42 30 610
Fragments arranged by size:
610 404 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 601 604 878 1116
Size: 598 3 3 274 238
Fragments arranged by size:
598 274 238 3 3
BseYI C'CCAG_C
Cuts at: 0 577 906 1097 1116
Size: 577 329 191 19
Fragments arranged by size:
577 329 191 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1116
Size: 473 643
BsiEI CG_ry'CG
Cuts at: 0 68 206 1116
Size: 68 138 910
Fragments arranged by size:
910 138 68
BsiHKAI G_wGCw'C
Cuts at: 0 825 1116
Size: 825 291
Bsp1286I G_dGCh'C
Cuts at: 0 103 199 657 825 1116
Size: 103 96 458 168 291
Fragments arranged by size:
458 291 168 103 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 909 1051 1116
Size: 909 142 65
Fragments arranged by size:
909 142 65
BsrBI CCG'CTC
Cuts at: 0 602 1116
Size: 602 514
BsrFI r'CCGG_y
Cuts at: 0 229 695 837 1004 1116
Size: 229 466 142 167 112
Fragments arranged by size:
466 229 167 142 112
BsrGI T'GTAC_A
Cuts at: 0 351 1116
Size: 351 765
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 1116
Size: 460 656
BtgI C'CryG_G
Cuts at: 0 71 128 191 200 382 555 978 1116
Size: 71 57 63 9 182 173 423 138
Fragments arranged by size:
423 182 173 138 71 63 57 9
BtsI GCAGTG_nn'
Cuts at: 0 405 615 1113 1116
Size: 405 210 498 3
Fragments arranged by size:
498 405 210 3
EaeI y'GGCC_r
Cuts at: 0 65 203 1116
Size: 65 138 913
Fragments arranged by size:
913 138 65
EagI C'GGCC_G
Cuts at: 0 65 203 1116
Size: 65 138 913
Fragments arranged by size:
913 138 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 944 1116
Size: 814 130 172
Fragments arranged by size:
814 172 130
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 494 1116
Size: 46 448 622
Fragments arranged by size:
622 448 46
EcoO109I rG'GnC_Cy
Cuts at: 0 84 653 883 1116
Size: 84 569 230 233
Fragments arranged by size:
569 233 230 84
EcoRV GAT'ATC
Cuts at: 0 985 1116
Size: 985 131
FspI TGC'GCA
Cuts at: 0 397 1116
Size: 397 719
HaeII r_GCGC'y
Cuts at: 0 142 525 555 1116
Size: 142 383 30 561
Fragments arranged by size:
561 383 142 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 1116
Size: 730 32 354
Fragments arranged by size:
730 354 32
KasI G'GCGC_C
Cuts at: 0 138 521 551 1116
Size: 138 383 30 565
Fragments arranged by size:
565 383 138 30
KpnI G_GTAC'C
Cuts at: 0 873 1116
Size: 873 243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 877 894 1116
Size: 79 798 17 222
Fragments arranged by size:
798 222 79 17
MspA1I CmG'CkG
Cuts at: 0 73 113 130 202 666 702 1116
Size: 73 40 17 72 464 36 414
Fragments arranged by size:
464 414 73 72 40 36 17
NaeI GCC'GGC
Cuts at: 0 231 1116
Size: 231 885
NarI GG'CG_CC
Cuts at: 0 139 522 552 1116
Size: 139 383 30 564
Fragments arranged by size:
564 383 139 30
NgoMIV G'CCGG_C
Cuts at: 0 229 1116
Size: 229 887
NotI GC'GGCC_GC
Cuts at: 0 65 203 1116
Size: 65 138 913
Fragments arranged by size:
913 138 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 913 1116
Size: 913 203
PmlI CAC'GTG
Cuts at: 0 723 1116
Size: 723 393
PpuMI rG'GwC_Cy
Cuts at: 0 84 883 1116
Size: 84 799 233
Fragments arranged by size:
799 233 84
PsiI TTA'TAA
Cuts at: 0 319 1116
Size: 319 797
PspOMI G'GGCC_C
Cuts at: 0 653 1116
Size: 653 463
PstI C_TGCA'G
Cuts at: 0 516 1116
Size: 516 600
PvuII CAG'CTG
Cuts at: 0 666 1116
Size: 666 450
SacII CC_GC'GG
Cuts at: 0 74 131 203 1116
Size: 74 57 72 913
Fragments arranged by size:
913 74 72 57
SfcI C'TryA_G
Cuts at: 0 419 512 1116
Size: 419 93 604
Fragments arranged by size:
604 419 93
SfoI GGC'GCC
Cuts at: 0 140 523 553 1116
Size: 140 383 30 563
Fragments arranged by size:
563 383 140 30
StyI C'CwwG_G
Cuts at: 0 273 865 1055 1116
Size: 273 592 190 61
Fragments arranged by size:
592 273 190 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1107 1111 1116
Size: 1107 4 5
Fragments arranged by size:
1107 5 4
TatI w'GTAC_w
Cuts at: 0 351 1116
Size: 351 765
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 476 508 939 971 1116
Size: 476 32 431 32 145
Fragments arranged by size:
476 431 145 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 1116
Size: 102 1014
Enzymes that do cut:
AarI Acc65I AcuI AgeI AlwNI ApaI BanI BanII
BbeI BbsI BglI Bme1580I BmgBI BmrI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BsrGI BstAPI BtgI BtsI EaeI
EagI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I
KasI KpnI MmeI MspA1I NaeI NarI NgoMIV NotI
PflMI PmlI PpuMI PsiI PspOMI PstI PvuII SacII
SfcI SfoI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoRI FalI FseI FspAI HincII HindIII HpaI MfeI
MluI MscI MslI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PpiI PshAI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI