Restriction map for
pFN21AB7608
(Linear) (Six-base) MAPSORT of: KIBB7608.seq Check: 8957 from: 1 to: 1071
KIBB7608.orf
With 182 enzymes: SgfI *
December 17, 2010 16:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 145 1071
Size: 145 926
Acc65I G'GTAC_C
Cuts at: 0 1007 1071
Size: 1007 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 1071
Size: 87 984
AlwNI CAG_nnn'CTG
Cuts at: 0 1032 1071
Size: 1032 39
ApaI G_GGCC'C
Cuts at: 0 360 1071
Size: 360 711
AseI AT'TA_AT
Cuts at: 0 992 1071
Size: 992 79
AvaI C'yCGr_G
Cuts at: 0 935 1071
Size: 935 136
BanI G'GyrC_C
Cuts at: 0 179 376 1007 1071
Size: 179 197 631 64
Fragments arranged by size:
631 197 179 64
BanII G_rGCy'C
Cuts at: 0 360 690 983 1071
Size: 360 330 293 88
Fragments arranged by size:
360 330 293 88
BbeI G_GCGC'C
Cuts at: 0 183 1071
Size: 183 888
BbsI GAAGACnn'nnnn_
Cuts at: 0 534 569 1071
Size: 534 35 502
Fragments arranged by size:
534 502 35
BbvCI CC'TCA_GC
Cuts at: 0 22 1071
Size: 22 1049
BclI T'GATC_A
Cuts at: 0 870 1071
Size: 870 201
BglI GCCn_nnn'nGGC
Cuts at: 0 374 1071
Size: 374 697
BglII A'GATC_T
Cuts at: 0 569 1061 1071
Size: 569 492 10
Fragments arranged by size:
569 492 10
BlpI GC'TnA_GC
Cuts at: 0 977 1071
Size: 977 94
Bme1580I G_kGCm'C
Cuts at: 0 297 360 379 1071
Size: 297 63 19 692
Fragments arranged by size:
692 297 63 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 866 1039 1071
Size: 395 471 173 32
Fragments arranged by size:
471 395 173 32
Bpu10I CC'TnA_GC
Cuts at: 0 22 1071
Size: 22 1049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1071
Size: 565 506
BsaHI Gr'CG_yC
Cuts at: 0 180 1071
Size: 180 891
BsaWI w'CCGG_w
Cuts at: 0 341 983 1071
Size: 341 642 88
Fragments arranged by size:
642 341 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 578 586 608 677 707 755 785
Size: 556 22 8 22 69 30 48 30
Cuts at: 785 1071
Size: 286
Fragments arranged by size:
556 286 69 48 30 30 22 22
8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1071
Size: 898 173
BseYI C'CCAG_C
Cuts at: 0 189 284 1071
Size: 189 95 787
Fragments arranged by size:
787 189 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1071
Size: 418 653
BsiHKAI G_wGCw'C
Cuts at: 0 794 983 1071
Size: 794 189 88
Fragments arranged by size:
794 189 88
Bsp1286I G_dGCh'C
Cuts at: 0 297 360 379 690 794 983 1071
Size: 297 63 19 311 104 189 88
Fragments arranged by size:
311 297 189 104 88 63 19
BspEI T'CCGG_A
Cuts at: 0 341 983 1071
Size: 341 642 88
Fragments arranged by size:
642 341 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 188 542 1071
Size: 145 43 354 529
Fragments arranged by size:
529 354 145 43
BsrDI GCAATG_nn'
Cuts at: 0 262 1071
Size: 262 809
BstYI r'GATC_y
Cuts at: 0 569 1061 1071
Size: 569 492 10
Fragments arranged by size:
569 492 10
BtgI C'CryG_G
Cuts at: 0 747 803 1071
Size: 747 56 268
Fragments arranged by size:
747 268 56
BtsI GCAGTG_nn'
Cuts at: 0 183 1071
Size: 183 888
DrdI GACnn_nn'nnGTC
Cuts at: 0 538 1071
Size: 538 533
EaeI y'GGCC_r
Cuts at: 0 2 1071
Size: 2 1069
EarI CTCTTCn'nnn_
Cuts at: 0 326 668 817 1071
Size: 326 342 149 254
Fragments arranged by size:
342 326 254 149
EcoICRI GAG'CTC
Cuts at: 0 981 1071
Size: 981 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 395 866 1039 1071
Size: 87 308 471 173 32
Fragments arranged by size:
471 308 173 87 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 1071
Size: 580 491
EcoO109I rG'GnC_Cy
Cuts at: 0 356 531 932 1029 1071
Size: 356 175 401 97 42
Fragments arranged by size:
401 356 175 97 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 1071
Size: 117 954
HaeII r_GCGC'y
Cuts at: 0 183 1071
Size: 183 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 1071
Size: 556 32 483
Fragments arranged by size:
556 483 32
KasI G'GCGC_C
Cuts at: 0 179 1071
Size: 179 892
KpnI G_GTAC'C
Cuts at: 0 1011 1071
Size: 1011 60
MfeI C'AATT_G
Cuts at: 0 674 1071
Size: 674 397
MscI TGG'CCA
Cuts at: 0 4 1071
Size: 4 1067
MslI CAynn'nnrTG
Cuts at: 0 68 499 746 1071
Size: 68 431 247 325
Fragments arranged by size:
431 325 247 68
MspA1I CmG'CkG
Cuts at: 0 288 592 876 1050 1071
Size: 288 304 284 174 21
Fragments arranged by size:
304 288 284 174 21
NarI GG'CG_CC
Cuts at: 0 180 1071
Size: 180 891
NcoI C'CATG_G
Cuts at: 0 747 803 1071
Size: 747 56 268
Fragments arranged by size:
747 268 56
PfoI T'CCnGG_A
Cuts at: 0 1055 1071
Size: 1055 16
PpuMI rG'GwC_Cy
Cuts at: 0 531 1071
Size: 531 540
PspOMI G'GGCC_C
Cuts at: 0 356 1071
Size: 356 715
PstI C_TGCA'G
Cuts at: 0 199 770 1069 1071
Size: 199 571 299 2
Fragments arranged by size:
571 299 199 2
PvuII CAG'CTG
Cuts at: 0 592 876 1071
Size: 592 284 195
Fragments arranged by size:
592 284 195
SacI G_AGCT'C
Cuts at: 0 983 1071
Size: 983 88
SapI GCTCTTCn'nnn_
Cuts at: 0 326 1071
Size: 326 745
SbfI CC_TGCA'GG
Cuts at: 0 199 1071
Size: 199 872
SfcI C'TryA_G
Cuts at: 0 195 766 1065 1071
Size: 195 571 299 6
Fragments arranged by size:
571 299 195 6
SfoI GGC'GCC
Cuts at: 0 181 1071
Size: 181 890
SmaI CCC'GGG
Cuts at: 0 937 1071
Size: 937 134
SmlI C'TyrA_G
Cuts at: 0 580 1071
Size: 580 491
StuI AGG'CCT
Cuts at: 0 408 968 1000 1071
Size: 408 560 32 71
Fragments arranged by size:
560 408 71 32
StyI C'CwwG_G
Cuts at: 0 747 803 1071
Size: 747 56 268
Fragments arranged by size:
747 268 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 854 1071
Size: 854 217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 632 664 1071
Size: 578 32 22 32 407
Fragments arranged by size:
578 407 32 32 22
XmaI C'CCGG_G
Cuts at: 0 935 1071
Size: 935 136
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI AseI AvaI BanI
BanII BbeI BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI BstYI
BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI KpnI MfeI MscI
MslI MspA1I NarI NcoI PfoI PpuMI PspOMI PstI
PvuII SacI SapI SbfI SfcI SfoI SmaI SmlI
StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AsiSI AvrII
BaeI BamHI BcgI BciVI BfrBI BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XcmI XhoI XmnI ZraI