Restriction map for
pFN21AB7604
(Linear) (Six-base) MAPSORT of: KIBB7604.seq Check: 9302 from: 1 to: 1065
KIBB7604.orf
With 182 enzymes: SgfI *
July 3, 2012 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 826 1065
Size: 826 239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 886 1065
Size: 398 488 179
Fragments arranged by size:
488 398 179
AfeI AGC'GCT
Cuts at: 0 76 1065
Size: 76 989
AleI CACnn'nnGTG
Cuts at: 0 890 1065
Size: 890 175
ApaI G_GGCC'C
Cuts at: 0 317 463 620 1065
Size: 317 146 157 445
Fragments arranged by size:
445 317 157 146
AscI GG'CGCG_CC
Cuts at: 0 236 608 1065
Size: 236 372 457
Fragments arranged by size:
457 372 236
AvaI C'yCGr_G
Cuts at: 0 94 292 412 456 487 613 1065
Size: 94 198 120 44 31 126 452
Fragments arranged by size:
452 198 126 120 94 44 31
BanI G'GyrC_C
Cuts at: 0 106 169 342 451 702 1065
Size: 106 63 173 109 251 363
Fragments arranged by size:
363 251 173 109 106 63
BanII G_rGCy'C
Cuts at: 0 317 374 463 620 956 1065
Size: 317 57 89 157 336 109
Fragments arranged by size:
336 317 157 109 89 57
BbeI G_GCGC'C
Cuts at: 0 173 346 455 706 1065
Size: 173 173 109 251 359
Fragments arranged by size:
359 251 173 173 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 1065
Size: 283 34 748
Fragments arranged by size:
748 283 34
BglI GCCn_nnn'nGGC
Cuts at: 0 312 536 685 1065
Size: 312 224 149 380
Fragments arranged by size:
380 312 224 149
BlpI GC'TnA_GC
Cuts at: 0 526 1065
Size: 526 539
Bme1580I G_kGCm'C
Cuts at: 0 317 463 620 1065
Size: 317 146 157 445
Fragments arranged by size:
445 317 157 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 924 1065
Size: 924 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 778 1065
Size: 769 9 287
Fragments arranged by size:
769 287 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1065
Size: 424 641
BsaI GGTCTCn'nnnn_
Cuts at: 0 664 727 1065
Size: 664 63 338
Fragments arranged by size:
664 338 63
BsaHI Gr'CG_yC
Cuts at: 0 170 343 452 703 1065
Size: 170 173 109 251 362
Fragments arranged by size:
362 251 173 170 109
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 1065
Size: 715 350
BseYI C'CCAG_C
Cuts at: 0 648 1065
Size: 648 417
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1030 1065
Size: 307 723 35
Fragments arranged by size:
723 307 35
BsiEI CG_ry'CG
Cuts at: 0 330 354 677 780 1014 1065
Size: 330 24 323 103 234 51
Fragments arranged by size:
330 323 234 103 51 24
BsiHKAI G_wGCw'C
Cuts at: 0 956 1065
Size: 956 109
Bsp1286I G_dGCh'C
Cuts at: 0 317 374 463 620 956 1065
Size: 317 57 89 157 336 109
Fragments arranged by size:
336 317 157 109 89 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 826 1065
Size: 826 239
BsrBI CCG'CTC
Cuts at: 0 525 785 1065
Size: 525 260 280
Fragments arranged by size:
525 280 260
BsrFI r'CCGG_y
Cuts at: 0 986 1065
Size: 986 79
BssHII G'CGCG_C
Cuts at: 0 84 236 544 567 608 940 1065
Size: 84 152 308 23 41 332 125
Fragments arranged by size:
332 308 152 125 84 41 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 1065
Size: 156 909
BtgI C'CryG_G
Cuts at: 0 149 260 423 683 717 1065
Size: 149 111 163 260 34 348
Fragments arranged by size:
348 260 163 149 111 34
BtsI GCAGTG_nn'
Cuts at: 0 319 906 1065
Size: 319 587 159
Fragments arranged by size:
587 319 159
EaeI y'GGCC_r
Cuts at: 0 79 327 351 674 777 988 1065
Size: 79 248 24 323 103 211 77
Fragments arranged by size:
323 248 211 103 79 77 24
EagI C'GGCC_G
Cuts at: 0 327 351 674 777 1065
Size: 327 24 323 103 288
Fragments arranged by size:
327 323 288 103 24
EarI CTCTTCn'nnn_
Cuts at: 0 667 1065
Size: 667 398
EcoICRI GAG'CTC
Cuts at: 0 954 1065
Size: 954 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 769 778 886 1065
Size: 398 371 9 108 179
Fragments arranged by size:
398 371 179 108 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 1065
Size: 361 704
EcoO109I rG'GnC_Cy
Cuts at: 0 313 484 1065
Size: 313 171 581
Fragments arranged by size:
581 313 171
FspI TGC'GCA
Cuts at: 0 880 1065
Size: 880 185
HaeII r_GCGC'y
Cuts at: 0 7 78 173 257 346 455 706 1045
Size: 7 71 95 84 89 109 251 339
Cuts at: 1045 1065
Size: 20
Fragments arranged by size:
339 251 109 95 89 84 71 20
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 846 878 1065
Size: 846 32 187
Fragments arranged by size:
846 187 32
KasI G'GCGC_C
Cuts at: 0 169 342 451 702 1065
Size: 169 173 109 251 363
Fragments arranged by size:
363 251 173 169 109
MslI CAynn'nnrTG
Cuts at: 0 890 996 1065
Size: 890 106 69
Fragments arranged by size:
890 106 69
MspA1I CmG'CkG
Cuts at: 0 262 685 719 747 1065
Size: 262 423 34 28 318
Fragments arranged by size:
423 318 262 34 28
NarI GG'CG_CC
Cuts at: 0 170 343 452 703 1065
Size: 170 173 109 251 362
Fragments arranged by size:
362 251 173 170 109
NcoI C'CATG_G
Cuts at: 0 149 1065
Size: 149 916
NotI GC'GGCC_GC
Cuts at: 0 674 777 1065
Size: 674 103 288
Fragments arranged by size:
674 288 103
NspI r_CATG'y
Cuts at: 0 480 1065
Size: 480 585
PasI CC'CwG_GG
Cuts at: 0 693 1065
Size: 693 372
PpuMI rG'GwC_Cy
Cuts at: 0 484 1065
Size: 484 581
PspOMI G'GGCC_C
Cuts at: 0 313 459 616 1065
Size: 313 146 157 449
Fragments arranged by size:
449 313 157 146
PstI C_TGCA'G
Cuts at: 0 71 823 1065
Size: 71 752 242
Fragments arranged by size:
752 242 71
PvuI CG_AT'CG
Cuts at: 0 1014 1065
Size: 1014 51
RsrII CG'GwC_CG
Cuts at: 0 562 1065
Size: 562 503
SacI G_AGCT'C
Cuts at: 0 956 1065
Size: 956 109
SacII CC_GC'GG
Cuts at: 0 263 686 720 1065
Size: 263 423 34 345
Fragments arranged by size:
423 345 263 34
SbfI CC_TGCA'GG
Cuts at: 0 71 1065
Size: 71 994
SfcI C'TryA_G
Cuts at: 0 67 249 819 1065
Size: 67 182 570 246
Fragments arranged by size:
570 246 182 67
SfoI GGC'GCC
Cuts at: 0 171 344 453 704 1065
Size: 171 173 109 251 361
Fragments arranged by size:
361 251 173 171 109
SmaI CCC'GGG
Cuts at: 0 414 458 489 615 1065
Size: 414 44 31 126 450
Fragments arranged by size:
450 414 126 44 31
SmlI C'TyrA_G
Cuts at: 0 439 1065
Size: 439 626
SphI G_CATG'C
Cuts at: 0 480 1065
Size: 480 585
SrfI GCCC'GGGC
Cuts at: 0 615 1065
Size: 615 450
StyI C'CwwG_G
Cuts at: 0 149 1065
Size: 149 916
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 1065
Size: 646 419
XmaI C'CCGG_G
Cuts at: 0 412 456 487 613 1065
Size: 412 44 31 126 452
Fragments arranged by size:
452 412 126 44 31
Enzymes that do cut:
AarI AcuI AfeI AleI ApaI AscI AvaI BanI
BanII BbeI BcgI BglI BlpI Bme1580I BmrI BpmI
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MslI MspA1I NarI NcoI NotI
NspI PasI PpuMI PspOMI PstI PvuI RsrII SacI
SacII SbfI SfcI SfoI SmaI SmlI SphI SrfI
StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NdeI NgoMIV NheI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuII SalI SanDI SapI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI