Restriction map for
pFN21AB7594
(Linear) (Six-base) MAPSORT of: KIBB7594.seq Check: 6163 from: 1 to: 873
KIBB7594.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AfeI AGC'GCT
Cuts at: 0 550 873
Size: 550 323
AlwNI CAG_nnn'CTG
Cuts at: 0 819 873
Size: 819 54
ApaI G_GGCC'C
Cuts at: 0 112 140 873
Size: 112 28 733
Fragments arranged by size:
733 112 28
AscI GG'CGCG_CC
Cuts at: 0 200 873
Size: 200 673
AvaI C'yCGr_G
Cuts at: 0 142 289 521 873
Size: 142 147 232 352
Fragments arranged by size:
352 232 147 142
BanI G'GyrC_C
Cuts at: 0 6 118 157 267 873
Size: 6 112 39 110 606
Fragments arranged by size:
606 112 110 39 6
BanII G_rGCy'C
Cuts at: 0 44 112 140 873
Size: 44 68 28 733
Fragments arranged by size:
733 68 44 28
BbeI G_GCGC'C
Cuts at: 0 10 122 161 873
Size: 10 112 39 712
Fragments arranged by size:
712 112 39 10
BglI GCCn_nnn'nGGC
Cuts at: 0 116 155 586 873
Size: 116 39 431 287
Fragments arranged by size:
431 287 116 39
BglII A'GATC_T
Cuts at: 0 530 873
Size: 530 343
Bme1580I G_kGCm'C
Cuts at: 0 112 140 344 873
Size: 112 28 204 529
Fragments arranged by size:
529 204 112 28
BmgBI CAC'GTC
Cuts at: 0 512 873
Size: 512 361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 873
Size: 336 537
Bpu10I CC'TnA_GC
Cuts at: 0 238 873
Size: 238 635
BsaI GGTCTCn'nnnn_
Cuts at: 0 354 370 873
Size: 354 16 503
Fragments arranged by size:
503 354 16
BsaHI Gr'CG_yC
Cuts at: 0 7 119 158 484 737 873
Size: 7 112 39 326 253 136
Fragments arranged by size:
326 253 136 112 39 7
BsaWI w'CCGG_w
Cuts at: 0 479 647 873
Size: 479 168 226
Fragments arranged by size:
479 226 168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 847 873
Size: 694 153 26
Fragments arranged by size:
694 153 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 772 873
Size: 547 225 101
Fragments arranged by size:
547 225 101
BsiEI CG_ry'CG
Cuts at: 0 428 873
Size: 428 445
BsmBI CGTCTCn'nnnn_
Cuts at: 0 176 873
Size: 176 697
Bsp1286I G_dGCh'C
Cuts at: 0 44 112 140 344 873
Size: 44 68 28 204 529
Fragments arranged by size:
529 204 68 44 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 873
Size: 615 258
BsrBI CCG'CTC
Cuts at: 0 684 696 780 873
Size: 684 12 84 93
Fragments arranged by size:
684 93 84 12
BsrFI r'CCGG_y
Cuts at: 0 270 710 873
Size: 270 440 163
Fragments arranged by size:
440 270 163
BssHII G'CGCG_C
Cuts at: 0 17 200 317 873
Size: 17 183 117 556
Fragments arranged by size:
556 183 117 17
BssSI C'ACGA_G
Cuts at: 0 37 873
Size: 37 836
BstAPI GCAn_nnn'nTGC
Cuts at: 0 819 873
Size: 819 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 873
Size: 129 744
BstYI r'GATC_y
Cuts at: 0 65 220 530 873
Size: 65 155 310 343
Fragments arranged by size:
343 310 155 65
BtgI C'CryG_G
Cuts at: 0 103 344 873
Size: 103 241 529
Fragments arranged by size:
529 241 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 541 873
Size: 541 332
DrdI GACnn_nn'nnGTC
Cuts at: 0 625 873
Size: 625 248
EaeI y'GGCC_r
Cuts at: 0 425 462 873
Size: 425 37 411
Fragments arranged by size:
425 411 37
EagI C'GGCC_G
Cuts at: 0 425 873
Size: 425 448
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 706 873
Size: 691 15 167
Fragments arranged by size:
691 167 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 873
Size: 336 537
EcoO109I rG'GnC_Cy
Cuts at: 0 108 109 136 137 873
Size: 108 1 27 1 736
Fragments arranged by size:
736 108 27 1 1
HaeII r_GCGC'y
Cuts at: 0 10 122 161 250 552 595 873
Size: 10 112 39 89 302 43 278
Fragments arranged by size:
302 278 112 89 43 39 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 873
Size: 205 32 636
Fragments arranged by size:
636 205 32
KasI G'GCGC_C
Cuts at: 0 6 118 157 873
Size: 6 112 39 716
Fragments arranged by size:
716 112 39 6
MspA1I CmG'CkG
Cuts at: 0 105 285 421 726 856 873
Size: 105 180 136 305 130 17
Fragments arranged by size:
305 180 136 130 105 17
NaeI GCC'GGC
Cuts at: 0 272 712 873
Size: 272 440 161
Fragments arranged by size:
440 272 161
NarI GG'CG_CC
Cuts at: 0 7 119 158 873
Size: 7 112 39 715
Fragments arranged by size:
715 112 39 7
NgoMIV G'CCGG_C
Cuts at: 0 270 710 873
Size: 270 440 163
Fragments arranged by size:
440 270 163
NotI GC'GGCC_GC
Cuts at: 0 425 873
Size: 425 448
PasI CC'CwG_GG
Cuts at: 0 827 873
Size: 827 46
PspOMI G'GGCC_C
Cuts at: 0 108 136 873
Size: 108 28 737
Fragments arranged by size:
737 108 28
PstI C_TGCA'G
Cuts at: 0 725 815 824 873
Size: 725 90 9 49
Fragments arranged by size:
725 90 49 9
PvuII CAG'CTG
Cuts at: 0 726 873
Size: 726 147
SacII CC_GC'GG
Cuts at: 0 106 873
Size: 106 767
SexAI A'CCwGG_T
Cuts at: 0 634 873
Size: 634 239
SfcI C'TryA_G
Cuts at: 0 721 811 820 873
Size: 721 90 9 53
Fragments arranged by size:
721 90 53 9
SfoI GGC'GCC
Cuts at: 0 8 120 159 873
Size: 8 112 39 714
Fragments arranged by size:
714 112 39 8
SmaI CCC'GGG
Cuts at: 0 144 291 523 873
Size: 144 147 232 350
Fragments arranged by size:
350 232 147 144
StyI C'CwwG_G
Cuts at: 0 357 845 873
Size: 357 488 28
Fragments arranged by size:
488 357 28
XmaI C'CCGG_G
Cuts at: 0 142 289 521 873
Size: 142 147 232 352
Fragments arranged by size:
352 232 147 142
Enzymes that do cut:
AfeI AlwNI ApaI AscI AvaI BanI BanII BbeI
BglI BglII Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI
BsaWI BseRI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI
DrdI EaeI EagI EciI Eco57MI EcoO109I HaeII Hin4I
KasI MspA1I NaeI NarI NgoMIV NotI PasI PspOMI
PstI PvuII SacII SexAI SfcI SfoI SmaI StyI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BlpI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaXI BseYI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII EarI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI