Restriction map for
pFN21AB7577
(Linear) (Six-base) MAPSORT of: KIBB7577.seq Check: 6798 from: 1 to: 654
KIBB7577.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 172 403 654
Size: 172 231 251
Fragments arranged by size:
251 231 172
ApaI G_GGCC'C
Cuts at: 0 67 654
Size: 67 587
ApoI r'AATT_y
Cuts at: 0 481 654
Size: 481 173
AvaI C'yCGr_G
Cuts at: 0 28 60 654
Size: 28 32 594
Fragments arranged by size:
594 32 28
BanI G'GyrC_C
Cuts at: 0 36 255 654
Size: 36 219 399
Fragments arranged by size:
399 219 36
BanII G_rGCy'C
Cuts at: 0 67 654
Size: 67 587
BbeI G_GCGC'C
Cuts at: 0 40 259 654
Size: 40 219 395
Fragments arranged by size:
395 219 40
BbvCI CC'TCA_GC
Cuts at: 0 610 654
Size: 610 44
BglI GCCn_nnn'nGGC
Cuts at: 0 55 654
Size: 55 599
BlpI GC'TnA_GC
Cuts at: 0 215 654
Size: 215 439
Bme1580I G_kGCm'C
Cuts at: 0 67 181 535 654
Size: 67 114 354 119
Fragments arranged by size:
354 119 114 67
BmgBI CAC'GTC
Cuts at: 0 318 654
Size: 318 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 182 654
Size: 87 95 472
Fragments arranged by size:
472 95 87
Bpu10I CC'TnA_GC
Cuts at: 0 468 610 654
Size: 468 142 44
Fragments arranged by size:
468 142 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 84 654
Size: 84 570
BsaAI yAC'GTr
Cuts at: 0 291 654
Size: 291 363
BsaHI Gr'CG_yC
Cuts at: 0 37 256 654
Size: 37 219 398
Fragments arranged by size:
398 219 37
BsaWI w'CCGG_w
Cuts at: 0 234 654
Size: 234 420
BseYI C'CCAG_C
Cuts at: 0 172 654
Size: 172 482
BsmBI CGTCTCn'nnnn_
Cuts at: 0 308 654
Size: 308 346
Bsp1286I G_dGCh'C
Cuts at: 0 67 181 535 654
Size: 67 114 354 119
Fragments arranged by size:
354 119 114 67
BsrBI CCG'CTC
Cuts at: 0 219 654
Size: 219 435
BsrFI r'CCGG_y
Cuts at: 0 39 654
Size: 39 615
BtgI C'CryG_G
Cuts at: 0 113 134 326 587 654
Size: 113 21 192 261 67
Fragments arranged by size:
261 192 113 67 21
BtsI GCAGTG_nn'
Cuts at: 0 404 516 654
Size: 404 112 138
Fragments arranged by size:
404 138 112
EaeI y'GGCC_r
Cuts at: 0 305 654
Size: 305 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 654
Size: 36 618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 182 654
Size: 87 95 472
Fragments arranged by size:
472 95 87
EcoO109I rG'GnC_Cy
Cuts at: 0 64 151 184 654
Size: 64 87 33 470
Fragments arranged by size:
470 87 64 33
HaeII r_GCGC'y
Cuts at: 0 40 259 654
Size: 40 219 395
Fragments arranged by size:
395 219 40
KasI G'GCGC_C
Cuts at: 0 36 255 654
Size: 36 219 399
Fragments arranged by size:
399 219 36
MslI CAynn'nnrTG
Cuts at: 0 628 654
Size: 628 26
MspA1I CmG'CkG
Cuts at: 0 11 115 136 145 169 214 366 654
Size: 11 104 21 9 24 45 152 288
Fragments arranged by size:
288 152 104 45 24 21 11 9
NaeI GCC'GGC
Cuts at: 0 41 654
Size: 41 613
NarI GG'CG_CC
Cuts at: 0 37 256 654
Size: 37 219 398
Fragments arranged by size:
398 219 37
NcoI C'CATG_G
Cuts at: 0 587 654
Size: 587 67
NgoMIV G'CCGG_C
Cuts at: 0 39 654
Size: 39 615
PfoI T'CCnGG_A
Cuts at: 0 550 654
Size: 550 104
PpuMI rG'GwC_Cy
Cuts at: 0 151 184 654
Size: 151 33 470
Fragments arranged by size:
470 151 33
PspOMI G'GGCC_C
Cuts at: 0 63 654
Size: 63 591
PstI C_TGCA'G
Cuts at: 0 10 654
Size: 10 644
PvuII CAG'CTG
Cuts at: 0 169 214 366 654
Size: 169 45 152 288
Fragments arranged by size:
288 169 152 45
SacII CC_GC'GG
Cuts at: 0 116 137 654
Size: 116 21 517
Fragments arranged by size:
517 116 21
SanDI GG'GwC_CC
Cuts at: 0 184 654
Size: 184 470
SfcI C'TryA_G
Cuts at: 0 6 511 654
Size: 6 505 143
Fragments arranged by size:
505 143 6
SfoI GGC'GCC
Cuts at: 0 38 257 654
Size: 38 219 397
Fragments arranged by size:
397 219 38
SmaI CCC'GGG
Cuts at: 0 62 654
Size: 62 592
SnaBI TAC'GTA
Cuts at: 0 291 654
Size: 291 363
StuI AGG'CCT
Cuts at: 0 436 654
Size: 436 218
StyI C'CwwG_G
Cuts at: 0 188 587 654
Size: 188 399 67
Fragments arranged by size:
399 188 67
TatI w'GTAC_w
Cuts at: 0 575 654
Size: 575 79
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 654
Size: 342 312
XmaI C'CCGG_G
Cuts at: 0 60 654
Size: 60 594
Enzymes that do cut:
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI
BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BseYI BsmBI Bsp1286I BsrBI BsrFI BtgI
BtsI EaeI EciI Eco57MI EcoO109I HaeII KasI MslI
MspA1I NaeI NarI NcoI NgoMIV PfoI PpuMI PspOMI
PstI PvuII SacII SanDI SfcI SfoI SmaI SnaBI
StuI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaBI
BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI