Restriction map for
pFN21AB7566
(Linear) (Six-base) MAPSORT of: KIBB7566.seq Check: 5158 from: 1 to: 831
KIBB7566.orf
With 182 enzymes: SgfI *
March 22, 2012 15:37 ..
AatII G_ACGT'C
Cuts at: 0 82 831
Size: 82 749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 831
Size: 694 137
ApaI G_GGCC'C
Cuts at: 0 284 526 831
Size: 284 242 305
Fragments arranged by size:
305 284 242
AscI GG'CGCG_CC
Cuts at: 0 371 831
Size: 371 460
AvaI C'yCGr_G
Cuts at: 0 15 160 465 831
Size: 15 145 305 366
Fragments arranged by size:
366 305 145 15
BanI G'GyrC_C
Cuts at: 0 123 335 831
Size: 123 212 496
Fragments arranged by size:
496 212 123
BanII G_rGCy'C
Cuts at: 0 254 284 526 831
Size: 254 30 242 305
Fragments arranged by size:
305 254 242 30
BbeI G_GCGC'C
Cuts at: 0 127 339 831
Size: 127 212 492
Fragments arranged by size:
492 212 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 82 831
Size: 82 749
BglI GCCn_nnn'nGGC
Cuts at: 0 46 295 806 831
Size: 46 249 511 25
Fragments arranged by size:
511 249 46 25
BglII A'GATC_T
Cuts at: 0 476 831
Size: 476 355
BlpI GC'TnA_GC
Cuts at: 0 31 831
Size: 31 800
Bme1580I G_kGCm'C
Cuts at: 0 42 284 526 831
Size: 42 242 242 305
Fragments arranged by size:
305 242 242 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 508 831
Size: 399 109 323
Fragments arranged by size:
399 323 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 831
Size: 748 83
BsaHI Gr'CG_yC
Cuts at: 0 79 124 336 419 634 662 824 831
Size: 79 45 212 83 215 28 162 7
Fragments arranged by size:
215 212 162 83 79 45 28 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 619 649 831
Size: 619 30 182
Fragments arranged by size:
619 182 30
BseYI C'CCAG_C
Cuts at: 0 132 552 831
Size: 132 420 279
Fragments arranged by size:
420 279 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 385 831
Size: 40 345 446
Fragments arranged by size:
446 345 40
BsiEI CG_ry'CG
Cuts at: 0 113 290 413 831
Size: 113 177 123 418
Fragments arranged by size:
418 177 123 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 544 831
Size: 544 287
Bsp1286I G_dGCh'C
Cuts at: 0 42 254 284 526 831
Size: 42 212 30 242 305
Fragments arranged by size:
305 242 212 42 30
BsrBI CCG'CTC
Cuts at: 0 159 831
Size: 159 672
BsrFI r'CCGG_y
Cuts at: 0 112 194 265 412 716 831
Size: 112 82 71 147 304 115
Fragments arranged by size:
304 147 115 112 82 71
BssHII G'CGCG_C
Cuts at: 0 146 298 371 531 533 831
Size: 146 152 73 160 2 298
Fragments arranged by size:
298 160 152 146 73 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 516 831
Size: 318 198 315
Fragments arranged by size:
318 315 198
BstYI r'GATC_y
Cuts at: 0 476 831
Size: 476 355
BtgI C'CryG_G
Cuts at: 0 200 284 831
Size: 200 84 547
Fragments arranged by size:
547 200 84
BtsI GCAGTG_nn'
Cuts at: 0 42 831
Size: 42 789
EaeI y'GGCC_r
Cuts at: 0 47 65 110 287 320 410 638 831
Size: 47 18 45 177 33 90 228 193
Fragments arranged by size:
228 193 177 90 47 45 33 18
EagI C'GGCC_G
Cuts at: 0 110 287 410 831
Size: 110 177 123 421
Fragments arranged by size:
421 177 123 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 640 831
Size: 252 388 191
Fragments arranged by size:
388 252 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 508 694 831
Size: 399 109 186 137
Fragments arranged by size:
399 186 137 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 831
Size: 427 404
EcoO109I rG'GnC_Cy
Cuts at: 0 138 164 219 384 831
Size: 138 26 55 165 447
Fragments arranged by size:
447 165 138 55 26
FseI GG_CCGG'CC
Cuts at: 0 198 831
Size: 198 633
HaeII r_GCGC'y
Cuts at: 0 127 339 793 831
Size: 127 212 454 38
Fragments arranged by size:
454 212 127 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 718 750 831
Size: 619 32 67 32 81
Fragments arranged by size:
619 81 67 32 32
KasI G'GCGC_C
Cuts at: 0 123 335 831
Size: 123 212 496
Fragments arranged by size:
496 212 123
MspA1I CmG'CkG
Cuts at: 0 45 286 292 831
Size: 45 241 6 539
Fragments arranged by size:
539 241 45 6
NaeI GCC'GGC
Cuts at: 0 114 196 267 414 831
Size: 114 82 71 147 417
Fragments arranged by size:
417 147 114 82 71
NarI GG'CG_CC
Cuts at: 0 124 336 831
Size: 124 212 495
Fragments arranged by size:
495 212 124
NgoMIV G'CCGG_C
Cuts at: 0 112 194 265 412 831
Size: 112 82 71 147 419
Fragments arranged by size:
419 147 112 82 71
NotI GC'GGCC_GC
Cuts at: 0 287 831
Size: 287 544
PflMI CCAn_nnn'nTGG
Cuts at: 0 350 831
Size: 350 481
PfoI T'CCnGG_A
Cuts at: 0 422 831
Size: 422 409
PpuMI rG'GwC_Cy
Cuts at: 0 164 384 831
Size: 164 220 447
Fragments arranged by size:
447 220 164
PshAI GACnn'nnGTC
Cuts at: 0 389 831
Size: 389 442
PspOMI G'GGCC_C
Cuts at: 0 280 522 831
Size: 280 242 309
Fragments arranged by size:
309 280 242
PstI C_TGCA'G
Cuts at: 0 104 831
Size: 104 727
SacII CC_GC'GG
Cuts at: 0 287 831
Size: 287 544
SanDI GG'GwC_CC
Cuts at: 0 164 384 831
Size: 164 220 447
Fragments arranged by size:
447 220 164
SfcI C'TryA_G
Cuts at: 0 100 831
Size: 100 731
SfoI GGC'GCC
Cuts at: 0 125 337 831
Size: 125 212 494
Fragments arranged by size:
494 212 125
SgrAI Cr'CCGG_yG
Cuts at: 0 265 831
Size: 265 566
SmaI CCC'GGG
Cuts at: 0 17 831
Size: 17 814
SmlI C'TyrA_G
Cuts at: 0 763 831
Size: 763 68
SrfI GCCC'GGGC
Cuts at: 0 17 831
Size: 17 814
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 831
Size: 577 254
XmaI C'CCGG_G
Cuts at: 0 15 831
Size: 15 816
XmnI GAAnn'nnTTC
Cuts at: 0 496 831
Size: 496 335
ZraI GAC'GTC
Cuts at: 0 80 831
Size: 80 751
Enzymes that do cut:
AatII AcuI ApaI AscI AvaI BanI BanII BbeI
BbsI BglI BglII BlpI Bme1580I BpmI BpuEI BsaHI
BsaXI BseYI BsgI BsiEI BsmBI Bsp1286I BsrBI BsrFI
BssHII BstXI BstYI BtgI BtsI EaeI EagI EciI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MspA1I
NaeI NarI NgoMIV NotI PflMI PfoI PpuMI PshAI
PspOMI PstI SacII SanDI SfcI SfoI SgrAI SmaI
SmlI SrfI TaqII XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaWI BseRI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SphI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI