Restriction map for
pFN21AB7544
(Linear) (Six-base) MAPSORT of: KIBB7544.seq Check: 3811 from: 1 to: 786
KIBB7544.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 786
Size: 24 762
AhdI GACnn_n'nnGTC
Cuts at: 0 438 786
Size: 438 348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 786
Size: 520 266
AlwNI CAG_nnn'CTG
Cuts at: 0 342 522 678 786
Size: 342 180 156 108
Fragments arranged by size:
342 180 156 108
AscI GG'CGCG_CC
Cuts at: 0 625 786
Size: 625 161
AvaI C'yCGr_G
Cuts at: 0 291 466 786
Size: 291 175 320
Fragments arranged by size:
320 291 175
BamHI G'GATC_C
Cuts at: 0 478 786
Size: 478 308
BanI G'GyrC_C
Cuts at: 0 580 786
Size: 580 206
BanII G_rGCy'C
Cuts at: 0 120 786
Size: 120 666
BbeI G_GCGC'C
Cuts at: 0 584 786
Size: 584 202
BbsI GAAGACnn'nnnn_
Cuts at: 0 57 401 582 786
Size: 57 344 181 204
Fragments arranged by size:
344 204 181 57
BglI GCCn_nnn'nGGC
Cuts at: 0 13 457 635 786
Size: 13 444 178 151
Fragments arranged by size:
444 178 151 13
BmtI G_CTAG'C
Cuts at: 0 335 786
Size: 335 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 304 442 786
Size: 31 273 138 344
Fragments arranged by size:
344 273 138 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 432 786
Size: 432 354
BsaHI Gr'CG_yC
Cuts at: 0 62 581 587 786
Size: 62 519 6 199
Fragments arranged by size:
519 199 62 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 591 786
Size: 403 188 195
Fragments arranged by size:
403 195 188
BseYI C'CCAG_C
Cuts at: 0 159 221 277 370 525 632 786
Size: 159 62 56 93 155 107 154
Fragments arranged by size:
159 155 154 107 93 62 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 786
Size: 2 784
BsmI GAATG_Cn'
Cuts at: 0 90 786
Size: 90 696
Bsp1286I G_dGCh'C
Cuts at: 0 120 786
Size: 120 666
BsrFI r'CCGG_y
Cuts at: 0 583 709 786
Size: 583 126 77
Fragments arranged by size:
583 126 77
BssHII G'CGCG_C
Cuts at: 0 625 682 684 758 786
Size: 625 57 2 74 28
Fragments arranged by size:
625 74 57 28 2
BssSI C'ACGA_G
Cuts at: 0 454 786
Size: 454 332
BstYI r'GATC_y
Cuts at: 0 478 716 786
Size: 478 238 70
Fragments arranged by size:
478 238 70
BtgI C'CryG_G
Cuts at: 0 317 786
Size: 317 469
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 786
Size: 766 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 31 304 442 786
Size: 24 7 273 138 344
Fragments arranged by size:
344 273 138 24 7
EcoO109I rG'GnC_Cy
Cuts at: 0 288 295 450 786
Size: 288 7 155 336
Fragments arranged by size:
336 288 155 7
HaeII r_GCGC'y
Cuts at: 0 584 786
Size: 584 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 786
Size: 424 32 330
Fragments arranged by size:
424 330 32
KasI G'GCGC_C
Cuts at: 0 580 786
Size: 580 206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 422 786
Size: 386 36 364
Fragments arranged by size:
386 364 36
MspA1I CmG'CkG
Cuts at: 0 44 83 632 786
Size: 44 39 549 154
Fragments arranged by size:
549 154 44 39
NaeI GCC'GGC
Cuts at: 0 585 786
Size: 585 201
NarI GG'CG_CC
Cuts at: 0 581 786
Size: 581 205
NdeI CA'TA_TG
Cuts at: 0 357 786
Size: 357 429
NgoMIV G'CCGG_C
Cuts at: 0 583 786
Size: 583 203
NheI G'CTAG_C
Cuts at: 0 331 786
Size: 331 455
NspI r_CATG'y
Cuts at: 0 552 786
Size: 552 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 678 786
Size: 342 336 108
Fragments arranged by size:
342 336 108
PstI C_TGCA'G
Cuts at: 0 258 774 786
Size: 258 516 12
Fragments arranged by size:
516 258 12
PvuII CAG'CTG
Cuts at: 0 44 83 786
Size: 44 39 703
Fragments arranged by size:
703 44 39
SbfI CC_TGCA'GG
Cuts at: 0 258 786
Size: 258 528
SfcI C'TryA_G
Cuts at: 0 246 254 770 786
Size: 246 8 516 16
Fragments arranged by size:
516 246 16 8
SfoI GGC'GCC
Cuts at: 0 582 786
Size: 582 204
SgrAI Cr'CCGG_yG
Cuts at: 0 583 786
Size: 583 203
SmaI CCC'GGG
Cuts at: 0 293 786
Size: 293 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 786
Size: 558 228
XmaI C'CCGG_G
Cuts at: 0 291 786
Size: 291 495
Enzymes that do cut:
AcuI AhdI AlfI AlwNI AscI AvaI BamHI BanI
BanII BbeI BbsI BglI BmtI BpmI BsaI BsaHI
BseRI BseYI BsgI BsmI Bsp1286I BsrFI BssHII BssSI
BstYI BtgI EciI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NspI
PflMI PstI PvuII SbfI SfcI SfoI SgrAI SmaI
TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI ApoI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI