Restriction map for
pFN21AB7504
(Linear) (Six-base) MAPSORT of: KIBB7504.seq Check: 3998 from: 1 to: 714
KIBB7504.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AfeI AGC'GCT
Cuts at: 0 361 714
Size: 361 353
AflIII A'CryG_T
Cuts at: 0 38 714
Size: 38 676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 443 714
Size: 443 271
AlwNI CAG_nnn'CTG
Cuts at: 0 27 156 390 445 714
Size: 27 129 234 55 269
Fragments arranged by size:
269 234 129 55 27
ApaI G_GGCC'C
Cuts at: 0 592 598 668 714
Size: 592 6 70 46
Fragments arranged by size:
592 70 46 6
AvaI C'yCGr_G
Cuts at: 0 90 591 661 714
Size: 90 501 70 53
Fragments arranged by size:
501 90 70 53
BanI G'GyrC_C
Cuts at: 0 13 714
Size: 13 701
BanII G_rGCy'C
Cuts at: 0 112 592 598 668 714
Size: 112 480 6 70 46
Fragments arranged by size:
480 112 70 46 6
BbeI G_GCGC'C
Cuts at: 0 17 714
Size: 17 697
BclI T'GATC_A
Cuts at: 0 32 714
Size: 32 682
BglI GCCn_nnn'nGGC
Cuts at: 0 457 550 714
Size: 457 93 164
Fragments arranged by size:
457 164 93
BlpI GC'TnA_GC
Cuts at: 0 106 301 714
Size: 106 195 413
Fragments arranged by size:
413 195 106
Bme1580I G_kGCm'C
Cuts at: 0 592 598 668 714
Size: 592 6 70 46
Fragments arranged by size:
592 70 46 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 632 714
Size: 632 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 714
Size: 85 629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 714
Size: 181 533
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 714
Size: 73 641
BsaHI Gr'CG_yC
Cuts at: 0 14 714
Size: 14 700
BseYI C'CCAG_C
Cuts at: 0 20 95 643 714
Size: 20 75 548 71
Fragments arranged by size:
548 75 71 20
BsiEI CG_ry'CG
Cuts at: 0 461 554 714
Size: 461 93 160
Fragments arranged by size:
461 160 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 56 714
Size: 56 658
Bsp1286I G_dGCh'C
Cuts at: 0 112 592 598 668 714
Size: 112 480 6 70 46
Fragments arranged by size:
480 112 70 46 6
BspHI T'CATG_A
Cuts at: 0 338 714
Size: 338 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 714
Size: 53 661
BsrBI CCG'CTC
Cuts at: 0 11 714
Size: 11 703
BsrFI r'CCGG_y
Cuts at: 0 187 493 568 714
Size: 187 306 75 146
Fragments arranged by size:
306 187 146 75
BssHII G'CGCG_C
Cuts at: 0 473 714
Size: 473 241
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 445 714
Size: 156 289 269
Fragments arranged by size:
289 269 156
BstYI r'GATC_y
Cuts at: 0 51 137 714
Size: 51 86 577
Fragments arranged by size:
577 86 51
BtgI C'CryG_G
Cuts at: 0 113 455 677 714
Size: 113 342 222 37
Fragments arranged by size:
342 222 113 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 180 714
Size: 180 534
EaeI y'GGCC_r
Cuts at: 0 458 542 551 583 680 714
Size: 458 84 9 32 97 34
Fragments arranged by size:
458 97 84 34 32 9
EagI C'GGCC_G
Cuts at: 0 458 551 714
Size: 458 93 163
Fragments arranged by size:
458 163 93
EarI CTCTTCn'nnn_
Cuts at: 0 76 714
Size: 76 638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 714
Size: 181 533
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 714
Size: 394 320
EcoO109I rG'GnC_Cy
Cuts at: 0 387 595 665 714
Size: 387 208 70 49
Fragments arranged by size:
387 208 70 49
HaeII r_GCGC'y
Cuts at: 0 17 363 714
Size: 17 346 351
Fragments arranged by size:
351 346 17
KasI G'GCGC_C
Cuts at: 0 13 714
Size: 13 701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 714
Size: 39 675
MslI CAynn'nnrTG
Cuts at: 0 425 714
Size: 425 289
MspA1I CmG'CkG
Cuts at: 0 105 115 131 144 442 457 507 610
Size: 105 10 16 13 298 15 50 103
Cuts at: 610 714
Size: 104
Fragments arranged by size:
298 105 104 103 50 16 15 13
10
NaeI GCC'GGC
Cuts at: 0 189 495 570 714
Size: 189 306 75 144
Fragments arranged by size:
306 189 144 75
NarI GG'CG_CC
Cuts at: 0 14 714
Size: 14 700
NgoMIV G'CCGG_C
Cuts at: 0 187 493 568 714
Size: 187 306 75 146
Fragments arranged by size:
306 187 146 75
NotI GC'GGCC_GC
Cuts at: 0 458 714
Size: 458 256
NspI r_CATG'y
Cuts at: 0 42 714
Size: 42 672
PciI A'CATG_T
Cuts at: 0 38 714
Size: 38 676
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 714
Size: 390 324
PspOMI G'GGCC_C
Cuts at: 0 588 594 664 714
Size: 588 6 70 50
Fragments arranged by size:
588 70 50 6
PvuII CAG'CTG
Cuts at: 0 144 442 714
Size: 144 298 272
Fragments arranged by size:
298 272 144
SacII CC_GC'GG
Cuts at: 0 116 458 714
Size: 116 342 256
Fragments arranged by size:
342 256 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 550 714
Size: 550 164
SfoI GGC'GCC
Cuts at: 0 15 714
Size: 15 699
SmaI CCC'GGG
Cuts at: 0 593 663 714
Size: 593 70 51
Fragments arranged by size:
593 70 51
SrfI GCCC'GGGC
Cuts at: 0 593 663 714
Size: 593 70 51
Fragments arranged by size:
593 70 51
StuI AGG'CCT
Cuts at: 0 400 714
Size: 400 314
XmaI C'CCGG_G
Cuts at: 0 591 661 714
Size: 591 70 53
Fragments arranged by size:
591 70 53
Enzymes that do cut:
AfeI AflIII AlfI AlwNI ApaI AvaI BanI BanII
BbeI BclI BglI BlpI Bme1580I BmrI BplI BpmI
BsaI BsaHI BseYI BsiEI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BstAPI BstYI BtgI BtgZI EaeI
EagI EarI Eco57MI EcoNI EcoO109I HaeII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PflMI PspOMI PvuII SacII SfiI SfoI SmaI SrfI
StuI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BglII BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiHKAI BsiWI BsmI BspEI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI