Restriction map for
pFN21AB7321
(Linear) (Six-base) MAPSORT of: KIBB7321.seq Check: 8291 from: 1 to: 3597
KIBB7321.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2430 3597
Size: 2430 1167
AccI GT'mk_AC
Cuts at: 0 673 2946 3597
Size: 673 2273 651
Fragments arranged by size:
2273 673 651
Acc65I G'GTAC_C
Cuts at: 0 486 3597
Size: 486 3111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1129 2566 3597
Size: 451 678 1437 1031
Fragments arranged by size:
1437 1031 678 451
AfeI AGC'GCT
Cuts at: 0 3384 3597
Size: 3384 213
AflIII A'CryG_T
Cuts at: 0 408 886 2595 2783 3597
Size: 408 478 1709 188 814
Fragments arranged by size:
1709 814 478 408 188
AleI CACnn'nnGTG
Cuts at: 0 1473 3597
Size: 1473 2124
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3094 3126 3597
Size: 3094 32 471
Fragments arranged by size:
3094 471 32
ApoI r'AATT_y
Cuts at: 0 114 549 613 865 878 1115 1399 1588
Size: 114 435 64 252 13 237 284 189
Cuts at: 1588 1921 2011 2118 2871 3597
Size: 333 90 107 753 726
Fragments arranged by size:
753 726 435 333 284 252 237 189
114 107 90 64 13
BamHI G'GATC_C
Cuts at: 0 1717 3597
Size: 1717 1880
BanI G'GyrC_C
Cuts at: 0 486 3597
Size: 486 3111
BanII G_rGCy'C
Cuts at: 0 23 3218 3597
Size: 23 3195 379
Fragments arranged by size:
3195 379 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 912 2257 3597
Size: 466 446 1345 1340
Fragments arranged by size:
1345 1340 466 446
BciVI GTATCCnnnnn_n'
Cuts at: 0 3138 3597
Size: 3138 459
BfrBI ATG'CAT
Cuts at: 0 786 3369 3597
Size: 786 2583 228
Fragments arranged by size:
2583 786 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 485 3297 3597
Size: 485 2812 300
Fragments arranged by size:
2812 485 300
BmtI G_CTAG'C
Cuts at: 0 3239 3597
Size: 3239 358
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 539 3597
Size: 539 3058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2006 2913 3194 3534 3597
Size: 2006 907 281 340 63
Fragments arranged by size:
2006 907 340 281 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3104 3597
Size: 3104 493
BsaAI yAC'GTr
Cuts at: 0 1680 3597
Size: 1680 1917
BsaBI GATnn'nnATC
Cuts at: 0 439 1841 3597
Size: 439 1402 1756
Fragments arranged by size:
1756 1402 439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 655 685 886 916 1817 1847 3597
Size: 655 30 201 30 901 30 1750
Fragments arranged by size:
1750 901 655 201 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1204 3142 3145 3597
Size: 1204 1938 3 452
Fragments arranged by size:
1938 1204 452 3
BseYI C'CCAG_C
Cuts at: 0 1052 3260 3597
Size: 1052 2208 337
Fragments arranged by size:
2208 1052 337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1723 2405 3597
Size: 1723 682 1192
Fragments arranged by size:
1723 1192 682
BsiHKAI G_wGCw'C
Cuts at: 0 525 2134 3461 3597
Size: 525 1609 1327 136
Fragments arranged by size:
1609 1327 525 136
Bsp1286I G_dGCh'C
Cuts at: 0 23 525 2134 3218 3461 3597
Size: 23 502 1609 1084 243 136
Fragments arranged by size:
1609 1084 502 243 136 23
BspHI T'CATG_A
Cuts at: 0 678 852 3153 3597
Size: 678 174 2301 444
Fragments arranged by size:
2301 678 444 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2430 3557 3597
Size: 2430 1127 40
Fragments arranged by size:
2430 1127 40
BsrDI GCAATG_nn'
Cuts at: 0 638 823 1859 2273 2728 2841 3597
Size: 638 185 1036 414 455 113 756
Fragments arranged by size:
1036 756 638 455 414 185 113
BsrGI T'GTAC_A
Cuts at: 0 598 3597
Size: 598 2999
BstAPI GCAn_nnn'nTGC
Cuts at: 0 343 637 1858 2113 3597
Size: 343 294 1221 255 1484
Fragments arranged by size:
1484 1221 343 294 255
BstYI r'GATC_y
Cuts at: 0 774 1717 1842 3597
Size: 774 943 125 1755
Fragments arranged by size:
1755 943 774 125
BstZ17I GTA'TAC
Cuts at: 0 674 3597
Size: 674 2923
BtsI GCAGTG_nn'
Cuts at: 0 1567 1732 2071 3597
Size: 1567 165 339 1526
Fragments arranged by size:
1567 1526 339 165
DraI TTT'AAA
Cuts at: 0 2622 3185 3597
Size: 2622 563 412
Fragments arranged by size:
2622 563 412
DraIII CAC_nnn'GTG
Cuts at: 0 1365 2649 3597
Size: 1365 1284 948
Fragments arranged by size:
1365 1284 948
EaeI y'GGCC_r
Cuts at: 0 746 2405 2640 3229 3597
Size: 746 1659 235 589 368
Fragments arranged by size:
1659 746 589 368 235
EarI CTCTTCn'nnn_
Cuts at: 0 2170 3597
Size: 2170 1427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1129 2006 2566 2913 3194 3534 3597
Size: 451 678 877 560 347 281 340 63
Fragments arranged by size:
877 678 560 451 347 340 281 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 359 2763 3597
Size: 359 2404 834
Fragments arranged by size:
2404 834 359
EcoO109I rG'GnC_Cy
Cuts at: 0 426 3597
Size: 426 3171
EcoRI G'AATT_C
Cuts at: 0 2871 3597
Size: 2871 726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 490 898 936 1378 2181 3597
Size: 490 408 38 442 803 1416
Fragments arranged by size:
1416 803 490 442 408 38
HaeII r_GCGC'y
Cuts at: 0 3386 3597
Size: 3386 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 571 603 1642 1674 3354 3386
Size: 212 32 327 32 1039 32 1680 32
Cuts at: 3386 3597
Size: 211
Fragments arranged by size:
1680 1039 327 212 211 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2789 3206 3597
Size: 2789 417 391
Fragments arranged by size:
2789 417 391
KpnI G_GTAC'C
Cuts at: 0 490 3597
Size: 490 3107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3072 3597
Size: 3072 525
MscI TGG'CCA
Cuts at: 0 748 2407 2642 3597
Size: 748 1659 235 955
Fragments arranged by size:
1659 955 748 235
MslI CAynn'nnrTG
Cuts at: 0 413 1473 2173 3452 3509 3597
Size: 413 1060 700 1279 57 88
Fragments arranged by size:
1279 1060 700 413 88 57
MspA1I CmG'CkG
Cuts at: 0 6 334 3376 3597
Size: 6 328 3042 221
Fragments arranged by size:
3042 328 221 6
NdeI CA'TA_TG
Cuts at: 0 1212 1472 3597
Size: 1212 260 2125
Fragments arranged by size:
2125 1212 260
NheI G'CTAG_C
Cuts at: 0 3235 3597
Size: 3235 362
NsiI A_TGCA'T
Cuts at: 0 788 3371 3597
Size: 788 2583 226
Fragments arranged by size:
2583 788 226
NspI r_CATG'y
Cuts at: 0 786 890 2599 2787 3192 3597
Size: 786 104 1709 188 405 405
Fragments arranged by size:
1709 786 405 405 188 104
PciI A'CATG_T
Cuts at: 0 886 2595 2783 3597
Size: 886 1709 188 814
Fragments arranged by size:
1709 886 814 188
PflMI CCAn_nnn'nTGG
Cuts at: 0 1047 2649 2974 3597
Size: 1047 1602 325 623
Fragments arranged by size:
1602 1047 623 325
PfoI T'CCnGG_A
Cuts at: 0 1662 3597
Size: 1662 1935
PpuMI rG'GwC_Cy
Cuts at: 0 426 3597
Size: 426 3171
PsiI TTA'TAA
Cuts at: 0 257 3597
Size: 257 3340
PstI C_TGCA'G
Cuts at: 0 339 2427 3597
Size: 339 2088 1170
Fragments arranged by size:
2088 1170 339
PvuII CAG'CTG
Cuts at: 0 334 3597
Size: 334 3263
ScaI AGT'ACT
Cuts at: 0 1443 3597
Size: 1443 2154
SfcI C'TryA_G
Cuts at: 0 335 722 2073 2245 2423 3413 3597
Size: 335 387 1351 172 178 990 184
Fragments arranged by size:
1351 990 387 335 184 178 172
SmlI C'TyrA_G
Cuts at: 0 3119 3597
Size: 3119 478
StuI AGG'CCT
Cuts at: 0 3166 3597
Size: 3166 431
StyI C'CwwG_G
Cuts at: 0 67 269 743 1046 3597
Size: 67 202 474 303 2551
Fragments arranged by size:
2551 474 303 202 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1092 3095 3303 3597
Size: 1092 2003 208 294
Fragments arranged by size:
2003 1092 294 208
TatI w'GTAC_w
Cuts at: 0 598 1441 2339 2921 3597
Size: 598 843 898 582 676
Fragments arranged by size:
898 843 676 598 582
XbaI T'CTAG_A
Cuts at: 0 1078 3597
Size: 1078 2519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 398 1060 2001 3597
Size: 398 662 941 1596
Fragments arranged by size:
1596 941 662 398
XmnI GAAnn'nnTTC
Cuts at: 0 697 878 2522 3597
Size: 697 181 1644 1075
Fragments arranged by size:
1644 1075 697 181
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AloI
ApoI BamHI BanI BanII BbsI BciVI BfrBI BmrI
BmtI BplI BpmI BpuEI BsaAI BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstYI BstZ17I BtsI DraI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NdeI NheI NsiI
NspI PciI PflMI PfoI PpuMI PsiI PstI PvuII
ScaI SfcI SmlI StuI StyI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
Bpu10I BsaI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI
Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NotI NruI
PacI PasI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmaI ZraI