Restriction map for
pFN21AB7280
(Linear) (Six-base) MAPSORT of: KIBB7280.seq Check: 9325 from: 1 to: 4008
KIBB7280.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 499 3434 4008
Size: 499 2935 574
Fragments arranged by size:
2935 574 499
AatII G_ACGT'C
Cuts at: 0 191 1544 3782 4008
Size: 191 1353 2238 226
Fragments arranged by size:
2238 1353 226 191
AccI GT'mk_AC
Cuts at: 0 2138 4008
Size: 2138 1870
Acc65I G'GTAC_C
Cuts at: 0 242 4008
Size: 242 3766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1033 1365 2289 2689 3760 4008
Size: 688 345 332 924 400 1071 248
Fragments arranged by size:
1071 924 688 400 345 332 248
AfeI AGC'GCT
Cuts at: 0 1881 4008
Size: 1881 2127
AflII C'TTAA_G
Cuts at: 0 1918 4008
Size: 1918 2090
AflIII A'CryG_T
Cuts at: 0 2141 4008
Size: 2141 1867
AhdI GACnn_n'nnGTC
Cuts at: 0 2134 4008
Size: 2134 1874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3700 3732 4008
Size: 3700 32 276
Fragments arranged by size:
3700 276 32
AlwNI CAG_nnn'CTG
Cuts at: 0 952 3627 3727 4008
Size: 952 2675 100 281
Fragments arranged by size:
2675 952 281 100
ApaI G_GGCC'C
Cuts at: 0 313 1048 1340 1367 1643 1796 1805 1859
Size: 313 735 292 27 276 153 9 54
Cuts at: 1859 2027 2332 2540 2632 2668 2947 2975 3031
Size: 168 305 208 92 36 279 28 56
Cuts at: 3031 3041 3050 4008
Size: 10 9 958
Fragments arranged by size:
958 735 313 305 292 279 276 208
168 153 92 56 54 36 28 27
10 9 9
ApaLI G'TGCA_C
Cuts at: 0 3118 3421 4008
Size: 3118 303 587
Fragments arranged by size:
3118 587 303
AvaI C'yCGr_G
Cuts at: 0 76 763 773 1095 1273 1457 1591 1608
Size: 76 687 10 322 178 184 134 17
Cuts at: 1608 1984 2124 2265 2376 2440 2599 2779 3130
Size: 376 140 141 111 64 159 180 351
Cuts at: 3130 3376 3745 4008
Size: 246 369 263
Fragments arranged by size:
687 376 369 351 322 263 246 184
180 178 159 141 140 134 111 76
64 17 10
BamHI G'GATC_C
Cuts at: 0 1072 1926 2158 4008
Size: 1072 854 232 1850
Fragments arranged by size:
1850 1072 854 232
BanI G'GyrC_C
Cuts at: 0 3 11 38 242 392 598 1195 1684
Size: 3 8 27 204 150 206 597 489
Cuts at: 1684 2320 2647 2936 3064 3589 3788 4008
Size: 636 327 289 128 525 199 220
Fragments arranged by size:
636 597 525 489 327 289 220 206
204 199 150 128 27 8 3
BanII G_rGCy'C
Cuts at: 0 313 608 697 764 930 1048 1340 1367
Size: 313 295 89 67 166 118 292 27
Cuts at: 1367 1643 1716 1761 1796 1805 1859 2027 2245
Size: 276 73 45 35 9 54 168 218
Cuts at: 2245 2332 2540 2632 2668 2780 2947 2975 3031
Size: 87 208 92 36 112 167 28 56
Cuts at: 3031 3041 3050 3170 3383 3402 4008
Size: 10 9 120 213 19 606
Fragments arranged by size:
606 313 295 292 276 218 213 208
168 167 166 120 118 112 92 89
87 73 67 56 54 45 36 35
28 27 19 10 9 9
BbeI G_GCGC'C
Cuts at: 0 7 42 602 1688 2651 2940 3068 4008
Size: 7 35 560 1086 963 289 128 940
Fragments arranged by size:
1086 963 940 560 289 128 35 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1241 4008
Size: 1241 2767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 744 778 2302 2336 4008
Size: 744 34 1524 34 1672
Fragments arranged by size:
1672 1524 744 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 33 1052 1061 1362 1505 1800 2003 2031
Size: 33 1019 9 301 143 295 203 28
Cuts at: 2031 2108 2315 2813 2822 2831 2840 2891 2951
Size: 77 207 498 9 9 9 51 60
Cuts at: 2951 2960 3026 3045 4008
Size: 9 66 19 963
Fragments arranged by size:
1019 963 498 301 295 207 203 143
77 66 60 51 33 28 19 9
9 9 9 9
BlpI GC'TnA_GC
Cuts at: 0 662 4008
Size: 662 3346
Bme1580I G_kGCm'C
Cuts at: 0 16 313 395 1048 1111 1340 1367 1643
Size: 16 297 82 653 63 229 27 276
Cuts at: 1643 1796 1805 1859 2027 2325 2332 2540 2632
Size: 153 9 54 168 298 7 208 92
Cuts at: 2632 2668 2947 2975 3031 3041 3050 3122 3425
Size: 36 279 28 56 10 9 72 303
Cuts at: 3425 3592 3668 3753 4008
Size: 167 76 85 255
Fragments arranged by size:
653 303 298 297 279 276 255 229
208 168 167 153 92 85 82 76
72 63 56 54 36 28 27 16
10 9 9 7
BmgBI CAC'GTC
Cuts at: 0 51 3869 4008
Size: 51 3818 139
Fragments arranged by size:
3818 139 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 1105 1664 4008
Size: 1105 559 2344
Fragments arranged by size:
2344 1105 559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3903 4008
Size: 3903 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 610 878 1265 1343 1459 2516 2534
Size: 604 6 268 387 78 116 1057 18
Cuts at: 2534 2591 2618 2787 3229 4008
Size: 57 27 169 442 779
Fragments arranged by size:
1057 779 604 442 387 268 169 116
78 57 27 18 6
Bpu10I CC'TnA_GC
Cuts at: 0 617 2167 4008
Size: 617 1550 1841
Fragments arranged by size:
1841 1550 617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3664 4008
Size: 3664 344
BsaI GGTCTCn'nnnn_
Cuts at: 0 1105 1307 2732 4008
Size: 1105 202 1425 1276
Fragments arranged by size:
1425 1276 1105 202
BsaAI yAC'GTr
Cuts at: 0 531 962 2142 2839 3420 4008
Size: 531 431 1180 697 581 588
Fragments arranged by size:
1180 697 588 581 531 431
BsaHI Gr'CG_yC
Cuts at: 0 4 39 188 599 770 1541 1685 2648
Size: 4 35 149 411 171 771 144 963
Cuts at: 2648 2937 3065 3779 4008
Size: 289 128 714 229
Fragments arranged by size:
963 771 714 411 289 229 171 149
144 128 35 4
BsaWI w'CCGG_w
Cuts at: 0 742 977 3194 4008
Size: 742 235 2217 814
Fragments arranged by size:
2217 814 742 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 803 833 1802 1832 3397 3427 3700 3730
Size: 803 30 969 30 1565 30 273 30
Cuts at: 3730 3867 3897 4008
Size: 137 30 111
Fragments arranged by size:
1565 969 803 273 137 111 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 556 805 913 3060 3178 3897 4008
Size: 133 423 249 108 2147 118 719 111
Fragments arranged by size:
2147 719 423 249 133 118 111 108
BseYI C'CCAG_C
Cuts at: 0 158 253 360 1137 2020 3108 3383 3700
Size: 158 95 107 777 883 1088 275 317
Cuts at: 3700 3704 3915 4008
Size: 4 211 93
Fragments arranged by size:
1088 883 777 317 275 211 158 107
95 93 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 449 3202 3265 3295 4008
Size: 403 46 2753 63 30 713
Fragments arranged by size:
2753 713 403 63 46 30
BsiEI CG_ry'CG
Cuts at: 0 2194 2395 2431 3433 3654 4008
Size: 2194 201 36 1002 221 354
Fragments arranged by size:
2194 1002 354 221 201 36
BsiHKAI G_wGCw'C
Cuts at: 0 56 930 1734 3122 3170 3314 3425 3488
Size: 56 874 804 1388 48 144 111 63
Cuts at: 3488 3974 4008
Size: 486 34
Fragments arranged by size:
1388 874 804 486 144 111 63 56
48 34
BsmI GAATG_Cn'
Cuts at: 0 560 2516 4008
Size: 560 1956 1492
Fragments arranged by size:
1956 1492 560
BsmBI CGTCTCn'nnnn_
Cuts at: 0 787 3859 4008
Size: 787 3072 149
Fragments arranged by size:
3072 787 149
Bsp1286I G_dGCh'C
Cuts at: 0 16 56 313 395 608 697 764 930
Size: 16 40 257 82 213 89 67 166
Cuts at: 930 1048 1111 1340 1367 1643 1716 1734 1761
Size: 118 63 229 27 276 73 18 27
Cuts at: 1761 1796 1805 1859 2027 2245 2325 2332 2540
Size: 35 9 54 168 218 80 7 208
Cuts at: 2540 2632 2668 2780 2947 2975 3031 3041 3050
Size: 92 36 112 167 28 56 10 9
Cuts at: 3050 3122 3170 3314 3383 3402 3425 3488 3592
Size: 72 48 144 69 19 23 63 104
Cuts at: 3592 3668 3753 3974 4008
Size: 76 85 221 34
Fragments arranged by size:
276 257 229 221 218 213 208 168
167 166 144 118 112 104 92 89
85 82 80 76 73 72 69 67
63 63 56 54 48 40 36 35
34 28 27 27 23 19 18 16
10 9 9 7
BspEI T'CCGG_A
Cuts at: 0 742 977 3194 4008
Size: 742 235 2217 814
Fragments arranged by size:
2217 814 742 235
BspHI T'CATG_A
Cuts at: 0 3992 4008
Size: 3992 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 499 1776 2226 3434 4008
Size: 153 346 1277 450 1208 574
Fragments arranged by size:
1277 1208 574 450 346 153
BsrBI CCG'CTC
Cuts at: 0 631 1745 4008
Size: 631 1114 2263
Fragments arranged by size:
2263 1114 631
BsrDI GCAATG_nn'
Cuts at: 0 2015 2580 4008
Size: 2015 565 1428
Fragments arranged by size:
2015 1428 565
BsrFI r'CCGG_y
Cuts at: 0 1555 2189 3432 3480 4008
Size: 1555 634 1243 48 528
Fragments arranged by size:
1555 1243 634 528 48
BsrGI T'GTAC_A
Cuts at: 0 3629 4008
Size: 3629 379
BssSI C'ACGA_G
Cuts at: 0 1733 3121 3230 4008
Size: 1733 1388 109 778
Fragments arranged by size:
1733 1388 778 109
BstEII G'GTnAC_C
Cuts at: 0 165 4008
Size: 165 3843
BstYI r'GATC_y
Cuts at: 0 1072 1417 1926 2070 2158 4008
Size: 1072 345 509 144 88 1850
Fragments arranged by size:
1850 1072 509 345 144 88
Bsu36I CC'TnA_GG
Cuts at: 0 2183 2906 3072 3784 4008
Size: 2183 723 166 712 224
Fragments arranged by size:
2183 723 712 224 166
BtgI C'CryG_G
Cuts at: 0 22 28 95 350 908 3059 3269 3301
Size: 22 6 67 255 558 2151 210 32
Cuts at: 3301 3537 3575 3893 4008
Size: 236 38 318 115
Fragments arranged by size:
2151 558 318 255 236 210 115 67
38 32 22 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 737 4008
Size: 737 3271
BtsI GCAGTG_nn'
Cuts at: 0 277 702 4008
Size: 277 425 3306
Fragments arranged by size:
3306 425 277
DrdI GACnn_nn'nnGTC
Cuts at: 0 187 973 2161 2378 2432 4008
Size: 187 786 1188 217 54 1576
Fragments arranged by size:
1576 1188 786 217 187 54
EaeI y'GGCC_r
Cuts at: 0 19 25 228 295 477 1053 2191 2392
Size: 19 6 203 67 182 576 1138 201
Cuts at: 2392 2428 3152 3251 3356 3430 3890 4008
Size: 36 724 99 105 74 460 118
Fragments arranged by size:
1138 724 576 460 203 201 182 118
105 99 74 67 36 19 6
EagI C'GGCC_G
Cuts at: 0 2191 2392 2428 3430 4008
Size: 2191 201 36 1002 578
Fragments arranged by size:
2191 1002 578 201 36
EarI CTCTTCn'nnn_
Cuts at: 0 265 613 1626 3802 4008
Size: 265 348 1013 2176 206
Fragments arranged by size:
2176 1013 348 265 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3888 4008
Size: 3888 120
EcoICRI GAG'CTC
Cuts at: 0 928 3168 4008
Size: 928 2240 840
Fragments arranged by size:
2240 928 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 610 688 878 1033 1265 1343 1365
Size: 604 6 78 190 155 232 78 22
Cuts at: 1365 1459 2289 2516 2534 2591 2618 2689 2787
Size: 94 830 227 18 57 27 71 98
Cuts at: 2787 3229 3760 4008
Size: 442 531 248
Fragments arranged by size:
830 604 531 442 248 232 227 190
155 98 94 78 78 71 57 27
22 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 712 4008
Size: 712 3296
EcoO109I rG'GnC_Cy
Cuts at: 0 147 309 579 681 1063 1126 1168 1177
Size: 147 162 270 102 382 63 42 9
Cuts at: 1177 1294 1336 1337 1345 1354 1363 1364 1372
Size: 117 42 1 8 9 9 1 8
Cuts at: 1372 1443 1453 1549 1585 1612 1621 1639 1640
Size: 71 10 96 36 27 9 18 1
Cuts at: 1640 1783 1793 1802 1855 1856 1897 2024 2128
Size: 143 10 9 53 1 41 127 104
Cuts at: 2128 2179 2224 2269 2329 2371 2527 2536 2537
Size: 51 45 45 60 42 156 9 1
Cuts at: 2537 2545 2628 2629 2638 2665 2860 2911 2943
Size: 8 83 1 9 27 195 51 32
Cuts at: 2943 2944 2962 2971 2972 3028 3038 3047 3624
Size: 1 18 9 1 56 10 9 577
Cuts at: 3624 4008
Size: 384
Fragments arranged by size:
577 384 382 270 195 162 156 147
143 127 117 104 102 96 83 71
63 60 56 53 51 51 45 45
42 42 42 41 36 32 27 27
18 18 10 10 10 9 9 9
9 9 9 9 9 9 8 8
8 1 1 1 1 1 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1416 4008
Size: 1416 2592
FseI GG_CCGG'CC
Cuts at: 0 2193 4008
Size: 2193 1815
FspI TGC'GCA
Cuts at: 0 676 4008
Size: 676 3332
HaeII r_GCGC'y
Cuts at: 0 7 42 602 1688 1883 2651 2940 3068
Size: 7 35 560 1086 195 768 289 128
Cuts at: 3068 4008
Size: 940
Fragments arranged by size:
1086 940 768 560 289 195 128 35
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 940 972 1802 1834 2120 2152
Size: 724 32 184 32 830 32 286 32
Cuts at: 2152 2844 2861 2876 2893 3867 3899 3939 3971
Size: 692 17 15 17 974 32 40 32
Cuts at: 3971 4008
Size: 37
Fragments arranged by size:
974 830 724 692 286 184 40 37
32 32 32 32 32 32 17 17
15
HincII GTy'rAC
Cuts at: 0 1868 3675 4008
Size: 1868 1807 333
Fragments arranged by size:
1868 1807 333
KasI G'GCGC_C
Cuts at: 0 3 38 598 1684 2647 2936 3064 4008
Size: 3 35 560 1086 963 289 128 944
Fragments arranged by size:
1086 963 944 560 289 128 35 3
KpnI G_GTAC'C
Cuts at: 0 246 4008
Size: 246 3762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1305 1562 1947 2126 2690 4008
Size: 1305 257 385 179 564 1318
Fragments arranged by size:
1318 1305 564 385 257 179
MscI TGG'CCA
Cuts at: 0 21 230 3358 4008
Size: 21 209 3128 650
Fragments arranged by size:
3128 650 209 21
MslI CAynn'nnrTG
Cuts at: 0 1408 3148 4008
Size: 1408 1740 860
Fragments arranged by size:
1740 1408 860
MspA1I CmG'CkG
Cuts at: 0 30 97 758 1825 3207 3279 3303 3539
Size: 30 67 661 1067 1382 72 24 236
Cuts at: 3539 3704 3738 4008
Size: 165 34 270
Fragments arranged by size:
1382 1067 661 270 236 165 72 67
34 30 24
NaeI GCC'GGC
Cuts at: 0 1557 2191 3434 4008
Size: 1557 634 1243 574
Fragments arranged by size:
1557 1243 634 574
NarI GG'CG_CC
Cuts at: 0 4 39 599 1685 2648 2937 3065 4008
Size: 4 35 560 1086 963 289 128 943
Fragments arranged by size:
1086 963 943 560 289 128 35 4
NcoI C'CATG_G
Cuts at: 0 22 350 3059 4008
Size: 22 328 2709 949
Fragments arranged by size:
2709 949 328 22
NgoMIV G'CCGG_C
Cuts at: 0 1555 2189 3432 4008
Size: 1555 634 1243 576
Fragments arranged by size:
1555 1243 634 576
NspI r_CATG'y
Cuts at: 0 3528 4008
Size: 3528 480
PasI CC'CwG_GG
Cuts at: 0 313 985 1122 1174 1318 1789 1798 2028
Size: 313 672 137 52 144 471 9 230
Cuts at: 2028 2325 2848 2968 3034 3043 4008
Size: 297 523 120 66 9 965
Fragments arranged by size:
965 672 523 471 313 297 230 144
137 120 66 52 9 9
PfoI T'CCnGG_A
Cuts at: 0 44 1476 1626 2211 2421 2607 2634 4008
Size: 44 1432 150 585 210 186 27 1374
Fragments arranged by size:
1432 1374 585 210 186 150 44 27
PmlI CAC'GTG
Cuts at: 0 962 2839 4008
Size: 962 1877 1169
Fragments arranged by size:
1877 1169 962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3700 3732 4008
Size: 3700 32 276
Fragments arranged by size:
3700 276 32
PpuMI rG'GwC_Cy
Cuts at: 0 147 681 1126 1168 1294 1345 1372 1453
Size: 147 534 445 42 126 51 27 81
Cuts at: 1453 1549 1585 1612 1897 2128 2179 2224 2269
Size: 96 36 27 285 231 51 45 45
Cuts at: 2269 2371 2527 2638 2860 2911 3624 4008
Size: 102 156 111 222 51 713 384
Fragments arranged by size:
713 534 445 384 285 231 222 156
147 126 111 102 96 81 51 51
51 45 45 42 36 27 27
PshAI GACnn'nnGTC
Cuts at: 0 1292 4008
Size: 1292 2716
PspOMI G'GGCC_C
Cuts at: 0 309 1044 1336 1363 1639 1792 1801 1855
Size: 309 735 292 27 276 153 9 54
Cuts at: 1855 2023 2328 2536 2628 2664 2943 2971 3027
Size: 168 305 208 92 36 279 28 56
Cuts at: 3027 3037 3046 4008
Size: 10 9 962
Fragments arranged by size:
962 735 309 305 292 279 276 208
168 153 92 56 54 36 28 27
10 9 9
PspXI vC'TCGA_Gb
Cuts at: 0 1457 4008
Size: 1457 2551
PstI C_TGCA'G
Cuts at: 0 137 1134 2223 3623 4008
Size: 137 997 1089 1400 385
Fragments arranged by size:
1400 1089 997 385 137
PvuII CAG'CTG
Cuts at: 0 3207 3279 3704 4008
Size: 3207 72 425 304
Fragments arranged by size:
3207 425 304 72
SacI G_AGCT'C
Cuts at: 0 930 3170 4008
Size: 930 2240 838
Fragments arranged by size:
2240 930 838
SacII CC_GC'GG
Cuts at: 0 31 98 3304 3540 4008
Size: 31 67 3206 236 468
Fragments arranged by size:
3206 468 236 67 31
SanDI GG'GwC_CC
Cuts at: 0 147 681 1585 1612 2269 2911 4008
Size: 147 534 904 27 657 642 1097
Fragments arranged by size:
1097 904 657 642 534 147 27
SapI GCTCTTCn'nnn_
Cuts at: 0 613 3802 4008
Size: 613 3189 206
Fragments arranged by size:
3189 613 206
SbfI CC_TGCA'GG
Cuts at: 0 1134 2223 3623 4008
Size: 1134 1089 1400 385
Fragments arranged by size:
1400 1134 1089 385
SexAI A'CCwGG_T
Cuts at: 0 423 1086 3491 4008
Size: 423 663 2405 517
Fragments arranged by size:
2405 663 517 423
SfcI C'TryA_G
Cuts at: 0 133 1130 2219 3619 4008
Size: 133 997 1089 1400 389
Fragments arranged by size:
1400 1089 997 389 133
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1052 1061 1362 1800 2031 2108 2822 2831
Size: 1052 9 301 438 231 77 714 9
Cuts at: 2831 2891 2951 2960 3026 3045 4008
Size: 60 60 9 66 19 963
Fragments arranged by size:
1052 963 714 438 301 231 77 66
60 60 19 9 9 9
SfoI GGC'GCC
Cuts at: 0 5 40 600 1686 2649 2938 3066 4008
Size: 5 35 560 1086 963 289 128 942
Fragments arranged by size:
1086 963 942 560 289 128 35 5
SmaI CCC'GGG
Cuts at: 0 775 1097 1275 1593 1610 1986 2126 2267
Size: 775 322 178 318 17 376 140 141
Cuts at: 2267 2378 2442 2601 2781 3747 4008
Size: 111 64 159 180 966 261
Fragments arranged by size:
966 775 376 322 318 261 180 178
159 141 140 111 64 17
SmlI C'TyrA_G
Cuts at: 0 1457 1918 3679 4008
Size: 1457 461 1761 329
Fragments arranged by size:
1761 1457 461 329
SphI G_CATG'C
Cuts at: 0 3528 4008
Size: 3528 480
StuI AGG'CCT
Cuts at: 0 1499 3904 4008
Size: 1499 2405 104
Fragments arranged by size:
2405 1499 104
StyI C'CwwG_G
Cuts at: 0 22 141 350 851 1298 1350 2228 2273
Size: 22 119 209 501 447 52 878 45
Cuts at: 2273 2348 2490 2642 2864 3059 4008
Size: 75 142 152 222 195 949
Fragments arranged by size:
949 878 501 447 222 209 195 152
142 119 75 52 45 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 557 1663 2756 3865 4008
Size: 184 373 1106 1093 1109 143
Fragments arranged by size:
1109 1106 1093 373 184 143
TatI w'GTAC_w
Cuts at: 0 3629 4008
Size: 3629 379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 1253 1285 3397 3429 4008
Size: 269 32 952 32 2112 32 579
Fragments arranged by size:
2112 952 579 269 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2086 4008
Size: 2086 1922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 705 849 2235 2658 4008
Size: 357 348 144 1386 423 1350
Fragments arranged by size:
1386 1350 423 357 348 144
XhoI C'TCGA_G
Cuts at: 0 1457 4008
Size: 1457 2551
XmaI C'CCGG_G
Cuts at: 0 773 1095 1273 1591 1608 1984 2124 2265
Size: 773 322 178 318 17 376 140 141
Cuts at: 2265 2376 2440 2599 2779 3745 4008
Size: 111 64 159 180 966 263
Fragments arranged by size:
966 773 376 322 318 263 180 178
159 141 140 111 64 17
XmnI GAAnn'nnTTC
Cuts at: 0 3190 3988 4008
Size: 3190 798 20
Fragments arranged by size:
3190 798 20
ZraI GAC'GTC
Cuts at: 0 189 1542 3780 4008
Size: 189 1353 2238 228
Fragments arranged by size:
2238 1353 228 189
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AleI AlfI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BclI BfrBI BglII BmtI
BsaBI BsiWI BssHII BstAPI BstBI BstXI BstZ17I ClaI
CspCI DraI DraIII EcoRI EcoRV FspAI HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PsiI PsrI PvuI RsrII SalI
ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XbaI