Restriction map for
pFN21AB7165
(Linear) (Six-base) MAPSORT of: KIBB7165.seq Check: 7189 from: 1 to: 1185
KIBB7165.orf
With 182 enzymes: SgfI *
March 22, 2012 15:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 384 928 1185
Size: 384 544 257
Fragments arranged by size:
544 384 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1185
Size: 40 1145
AflIII A'CryG_T
Cuts at: 0 341 788 1185
Size: 341 447 397
Fragments arranged by size:
447 397 341
AleI CACnn'nnGTG
Cuts at: 0 205 1185
Size: 205 980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 1185
Size: 649 536
ApaI G_GGCC'C
Cuts at: 0 782 1096 1153 1185
Size: 782 314 57 32
Fragments arranged by size:
782 314 57 32
AvaI C'yCGr_G
Cuts at: 0 570 669 825 1179 1185
Size: 570 99 156 354 6
Fragments arranged by size:
570 354 156 99 6
BanI G'GyrC_C
Cuts at: 0 298 489 648 1185
Size: 298 191 159 537
Fragments arranged by size:
537 298 191 159
BanII G_rGCy'C
Cuts at: 0 782 1096 1153 1185
Size: 782 314 57 32
Fragments arranged by size:
782 314 57 32
BglI GCCn_nnn'nGGC
Cuts at: 0 754 1048 1185
Size: 754 294 137
Fragments arranged by size:
754 294 137
BlpI GC'TnA_GC
Cuts at: 0 364 1168 1185
Size: 364 804 17
Fragments arranged by size:
804 364 17
Bme1580I G_kGCm'C
Cuts at: 0 301 471 494 782 1096 1153 1185
Size: 301 170 23 288 314 57 32
Fragments arranged by size:
314 301 288 170 57 32 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 1185
Size: 175 1010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 61 823 988 1185
Size: 43 18 762 165 197
Fragments arranged by size:
762 197 165 43 18
BsaAI yAC'GTr
Cuts at: 0 593 789 912 1185
Size: 593 196 123 273
Fragments arranged by size:
593 273 196 123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 312 1023 1096 1185
Size: 312 711 73 89
Fragments arranged by size:
711 312 89 73
BseYI C'CCAG_C
Cuts at: 0 132 294 773 996 1119 1185
Size: 132 162 479 223 123 66
Fragments arranged by size:
479 223 162 132 123 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 196 301 1185
Size: 97 99 105 884
Fragments arranged by size:
884 105 99 97
BsiEI CG_ry'CG
Cuts at: 0 104 406 683 711 749 838 1185
Size: 104 302 277 28 38 89 347
Fragments arranged by size:
347 302 277 104 89 38 28
BsiHKAI G_wGCw'C
Cuts at: 0 482 908 917 1185
Size: 482 426 9 268
Fragments arranged by size:
482 426 268 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 1108 1185
Size: 61 1047 77
Fragments arranged by size:
1047 77 61
Bsp1286I G_dGCh'C
Cuts at: 0 301 471 482 494 782 908 917 1096
Size: 301 170 11 12 288 126 9 179
Cuts at: 1096 1153 1185
Size: 57 32
Fragments arranged by size:
301 288 179 170 126 57 32 12
11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 384 928 1185
Size: 384 544 257
Fragments arranged by size:
544 384 257
BsrBI CCG'CTC
Cuts at: 0 840 1046 1185
Size: 840 206 139
Fragments arranged by size:
840 206 139
BsrFI r'CCGG_y
Cuts at: 0 405 713 1185
Size: 405 308 472
Fragments arranged by size:
472 405 308
BssSI C'ACGA_G
Cuts at: 0 285 841 1185
Size: 285 556 344
Fragments arranged by size:
556 344 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 399 542 1185
Size: 399 143 643
Fragments arranged by size:
643 399 143
BstEII G'GTnAC_C
Cuts at: 0 195 1185
Size: 195 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 1185
Size: 378 807
BtgI C'CryG_G
Cuts at: 0 191 275 431 437 705 1087 1185
Size: 191 84 156 6 268 382 98
Fragments arranged by size:
382 268 191 156 98 84 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 599 648 1185
Size: 599 49 537
Fragments arranged by size:
599 537 49
EaeI y'GGCC_r
Cuts at: 0 5 101 272 403 434 617 680 708
Size: 5 96 171 131 31 183 63 28
Cuts at: 708 718 746 755 835 893 1185
Size: 10 28 9 80 58 292
Fragments arranged by size:
292 183 171 131 96 80 63 58
31 28 28 10 9 5
EagI C'GGCC_G
Cuts at: 0 101 403 680 708 746 835 1185
Size: 101 302 277 28 38 89 350
Fragments arranged by size:
350 302 277 101 89 38 28
EarI CTCTTCn'nnn_
Cuts at: 0 166 1185
Size: 166 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 43 61 823 988 1185
Size: 40 3 18 762 165 197
Fragments arranged by size:
762 197 165 40 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 802 1185
Size: 802 383
EcoO109I rG'GnC_Cy
Cuts at: 0 574 778 779 1092 1093 1150 1185
Size: 574 204 1 313 1 57 35
Fragments arranged by size:
574 313 204 57 35 1 1
FspI TGC'GCA
Cuts at: 0 937 958 1131 1185
Size: 937 21 173 54
Fragments arranged by size:
937 173 54 21
HaeII r_GCGC'y
Cuts at: 0 223 371 951 1168 1185
Size: 223 148 580 217 17
Fragments arranged by size:
580 223 217 148 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 502 534 1185
Size: 39 32 431 32 651
Fragments arranged by size:
651 431 39 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1185
Size: 251 934
MscI TGG'CCA
Cuts at: 0 895 1185
Size: 895 290
MslI CAynn'nnrTG
Cuts at: 0 205 1185
Size: 205 980
MspA1I CmG'CkG
Cuts at: 0 353 386 439 654 707 1089 1127 1185
Size: 353 33 53 215 53 382 38 58
Fragments arranged by size:
382 353 215 58 53 53 38 33
NaeI GCC'GGC
Cuts at: 0 715 1185
Size: 715 470
NgoMIV G'CCGG_C
Cuts at: 0 713 1185
Size: 713 472
NotI GC'GGCC_GC
Cuts at: 0 708 835 1185
Size: 708 127 350
Fragments arranged by size:
708 350 127
NspI r_CATG'y
Cuts at: 0 183 345 933 1185
Size: 183 162 588 252
Fragments arranged by size:
588 252 183 162
PciI A'CATG_T
Cuts at: 0 341 1185
Size: 341 844
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 1185
Size: 192 993
PfoI T'CCnGG_A
Cuts at: 0 38 506 1185
Size: 38 468 679
Fragments arranged by size:
679 468 38
PmlI CAC'GTG
Cuts at: 0 593 789 1185
Size: 593 196 396
Fragments arranged by size:
593 396 196
PshAI GACnn'nnGTC
Cuts at: 0 193 1185
Size: 193 992
PspOMI G'GGCC_C
Cuts at: 0 778 1092 1149 1185
Size: 778 314 57 36
Fragments arranged by size:
778 314 57 36
PspXI vC'TCGA_Gb
Cuts at: 0 669 1179 1185
Size: 669 510 6
Fragments arranged by size:
669 510 6
PstI C_TGCA'G
Cuts at: 0 395 1072 1185
Size: 395 677 113
Fragments arranged by size:
677 395 113
PvuII CAG'CTG
Cuts at: 0 1127 1185
Size: 1127 58
SacII CC_GC'GG
Cuts at: 0 440 708 1090 1185
Size: 440 268 382 95
Fragments arranged by size:
440 382 268 95
SbfI CC_TGCA'GG
Cuts at: 0 395 1185
Size: 395 790
SfcI C'TryA_G
Cuts at: 0 391 1068 1185
Size: 391 677 117
Fragments arranged by size:
677 391 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 754 1185
Size: 754 431
SgrAI Cr'CCGG_yG
Cuts at: 0 713 1185
Size: 713 472
SmaI CCC'GGG
Cuts at: 0 572 827 1185
Size: 572 255 358
Fragments arranged by size:
572 358 255
SmlI C'TyrA_G
Cuts at: 0 669 1179 1185
Size: 669 510 6
Fragments arranged by size:
669 510 6
SphI G_CATG'C
Cuts at: 0 183 1185
Size: 183 1002
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 988 1185
Size: 195 793 197
Fragments arranged by size:
793 197 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 1185
Size: 522 663
XhoI C'TCGA_G
Cuts at: 0 669 1179 1185
Size: 669 510 6
Fragments arranged by size:
669 510 6
XmaI C'CCGG_G
Cuts at: 0 570 825 1185
Size: 570 255 360
Fragments arranged by size:
570 360 255
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI ApaI AvaI BanI
BanII BglI BlpI Bme1580I BplI BpmI BsaAI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BtgI BtgZI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NgoMIV NotI NspI
PciI PflMI PfoI PmlI PshAI PspOMI PspXI PstI
PvuII SacII SbfI SfcI SfiI SgrAI SmaI SmlI
SphI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NruI
NsiI PacI PasI PmeI PpiI PpuMI PsiI PsrI
PvuI RsrII SacI SalI SanDI SapI ScaI SexAI
SfoI SgfI SnaBI SpeI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XmnI ZraI