Restriction map for
pFN21AB7153
(Linear) (Six-base) MAPSORT of: KIBB7153.seq Check: 7356 from: 1 to: 1059
KIBB7153.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 732 1059
Size: 345 387 327
Fragments arranged by size:
387 345 327
AflIII A'CryG_T
Cuts at: 0 863 1059
Size: 863 196
AleI CACnn'nnGTG
Cuts at: 0 709 1059
Size: 709 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 332 463 1033 1059
Size: 332 131 570 26
Fragments arranged by size:
570 332 131 26
ApoI r'AATT_y
Cuts at: 0 837 1059
Size: 837 222
AvaI C'yCGr_G
Cuts at: 0 320 526 960 1059
Size: 320 206 434 99
Fragments arranged by size:
434 320 206 99
BanII G_rGCy'C
Cuts at: 0 331 1059
Size: 331 728
BbsI GAAGACnn'nnnn_
Cuts at: 0 351 1059
Size: 351 708
BbvCI CC'TCA_GC
Cuts at: 0 292 1059
Size: 292 767
BglI GCCn_nnn'nGGC
Cuts at: 0 616 1059
Size: 616 443
BmgBI CAC'GTC
Cuts at: 0 1025 1059
Size: 1025 34
BmtI G_CTAG'C
Cuts at: 0 688 1059
Size: 688 371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 108 552 1059
Size: 108 444 507
Fragments arranged by size:
507 444 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1021 1059
Size: 975 46 38
Fragments arranged by size:
975 46 38
Bpu10I CC'TnA_GC
Cuts at: 0 292 1059
Size: 292 767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 679 1059
Size: 636 43 380
Fragments arranged by size:
636 380 43
BsaAI yAC'GTr
Cuts at: 0 393 627 866 1059
Size: 393 234 239 193
Fragments arranged by size:
393 239 234 193
BsaHI Gr'CG_yC
Cuts at: 0 301 719 1059
Size: 301 418 340
Fragments arranged by size:
418 340 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1059
Size: 328 731
BsiEI CG_ry'CG
Cuts at: 0 904 922 1059
Size: 904 18 137
Fragments arranged by size:
904 137 18
BsiHKAI G_wGCw'C
Cuts at: 0 164 1059
Size: 164 895
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 79 831 1059
Size: 61 18 752 228
Fragments arranged by size:
752 228 61 18
Bsp1286I G_dGCh'C
Cuts at: 0 164 331 1059
Size: 164 167 728
Fragments arranged by size:
728 167 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 890 1059
Size: 890 169
BsrBI CCG'CTC
Cuts at: 0 650 1059
Size: 650 409
BsrDI GCAATG_nn'
Cuts at: 0 164 206 398 1059
Size: 164 42 192 661
Fragments arranged by size:
661 192 164 42
BssSI C'ACGA_G
Cuts at: 0 462 1059
Size: 462 597
BstAPI GCAn_nnn'nTGC
Cuts at: 0 570 638 1059
Size: 570 68 421
Fragments arranged by size:
570 421 68
BstEII G'GTnAC_C
Cuts at: 0 582 702 1059
Size: 582 120 357
Fragments arranged by size:
582 357 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 999 1038 1059
Size: 144 855 39 21
Fragments arranged by size:
855 144 39 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 1059
Size: 360 699
BtsI GCAGTG_nn'
Cuts at: 0 316 916 1059
Size: 316 600 143
Fragments arranged by size:
600 316 143
EaeI y'GGCC_r
Cuts at: 0 224 371 395 608 668 901 919 1059
Size: 224 147 24 213 60 233 18 140
Fragments arranged by size:
233 224 213 147 140 60 24 18
EagI C'GGCC_G
Cuts at: 0 901 919 1059
Size: 901 18 140
Fragments arranged by size:
901 140 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 732 975 1021 1059
Size: 345 387 243 46 38
Fragments arranged by size:
387 345 243 46 38
EcoO109I rG'GnC_Cy
Cuts at: 0 458 879 956 1059
Size: 458 421 77 103
Fragments arranged by size:
458 421 103 77
EcoRI G'AATT_C
Cuts at: 0 837 1059
Size: 837 222
FspI TGC'GCA
Cuts at: 0 631 1059
Size: 631 428
FspAI rTGC'GCAy
Cuts at: 0 631 1059
Size: 631 428
HaeII r_GCGC'y
Cuts at: 0 390 1059
Size: 390 669
MscI TGG'CCA
Cuts at: 0 373 397 610 1059
Size: 373 24 213 449
Fragments arranged by size:
449 373 213 24
MslI CAynn'nnrTG
Cuts at: 0 142 169 709 1015 1059
Size: 142 27 540 306 44
Fragments arranged by size:
540 306 142 44 27
MspA1I CmG'CkG
Cuts at: 0 906 1059
Size: 906 153
NheI G'CTAG_C
Cuts at: 0 684 1059
Size: 684 375
NspI r_CATG'y
Cuts at: 0 444 1014 1020 1059
Size: 444 570 6 39
Fragments arranged by size:
570 444 39 6
PfoI T'CCnGG_A
Cuts at: 0 41 1059
Size: 41 1018
PmlI CAC'GTG
Cuts at: 0 393 866 1059
Size: 393 473 193
Fragments arranged by size:
473 393 193
PpuMI rG'GwC_Cy
Cuts at: 0 879 956 1059
Size: 879 77 103
Fragments arranged by size:
879 103 77
PspXI vC'TCGA_Gb
Cuts at: 0 526 1059
Size: 526 533
PstI C_TGCA'G
Cuts at: 0 911 1059
Size: 911 148
SanDI GG'GwC_CC
Cuts at: 0 956 1059
Size: 956 103
SfcI C'TryA_G
Cuts at: 0 907 1059
Size: 907 152
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 616 1059
Size: 616 443
SmaI CCC'GGG
Cuts at: 0 322 962 1059
Size: 322 640 97
Fragments arranged by size:
640 322 97
SmlI C'TyrA_G
Cuts at: 0 526 651 694 1059
Size: 526 125 43 365
Fragments arranged by size:
526 365 125 43
SphI G_CATG'C
Cuts at: 0 444 1014 1059
Size: 444 570 45
Fragments arranged by size:
570 444 45
StuI AGG'CCT
Cuts at: 0 532 1059
Size: 532 527
StyI C'CwwG_G
Cuts at: 0 62 404 501 1059
Size: 62 342 97 558
Fragments arranged by size:
558 342 97 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1044 1059
Size: 1044 15
TatI w'GTAC_w
Cuts at: 0 11 1059
Size: 11 1048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 603 1059
Size: 69 534 456
Fragments arranged by size:
534 456 69
XhoI C'TCGA_G
Cuts at: 0 526 1059
Size: 526 533
XmaI C'CCGG_G
Cuts at: 0 320 960 1059
Size: 320 640 99
Fragments arranged by size:
640 320 99
XmnI GAAnn'nnTTC
Cuts at: 0 152 1059
Size: 152 907
Enzymes that do cut:
AcuI AflIII AleI AlfI ApoI AvaI BanII BbsI
BbvCI BglI BmgBI BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BssSI BstAPI BstEII BstXI BtgZI BtsI
EaeI EagI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
MscI MslI MspA1I NheI NspI PfoI PmlI PpuMI
PspXI PstI SanDI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsiWI BsmI BspEI BspHI BsrFI
BsrGI BssHII BstBI BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoRV FalI FseI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI ZraI