(Linear) (Six-base) MAPSORT of: KIBB7141.seq  Check: 8983  from: 1  to: 1011



KIBB7141.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     325    1011 
   Size:        325     686
 
AccI GT'mk_AC

Cuts at:      0     350     617     976    1011 
   Size:        350     267     359      35

  Fragments arranged by size: 

                 359     350     267      35
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144    1011 
   Size:        144     867
 
AflIII A'CryG_T

Cuts at:      0      10     562    1011 
   Size:         10     552     449

  Fragments arranged by size: 

                 552     449      10
 
ApoI r'AATT_y

Cuts at:      0     149     288     916    1011 
   Size:        149     139     628      95

  Fragments arranged by size: 

                 628     149     139      95
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     782    1011 
   Size:        782     229
 
BfrBI ATG'CAT

Cuts at:      0     190    1011 
   Size:        190     821
 
BmgBI CAC'GTC

Cuts at:      0     563    1011 
   Size:        563     448
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     935    1011 
   Size:        935      76
 
BsaWI w'CCGG_w

Cuts at:      0     608    1011 
   Size:        608     403
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     231     261    1011 
   Size:        231      30     750

  Fragments arranged by size: 

                 750     231      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     906    1011 
   Size:        906     105
 
BsmI GAATG_Cn'

Cuts at:      0      83     307    1011 
   Size:         83     224     704

  Fragments arranged by size: 

                 704     224      83
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     325    1011 
   Size:        325     686
 
BsrDI GCAATG_nn'

Cuts at:      0     320    1011 
   Size:        320     691
 
BsrGI T'GTAC_A

Cuts at:      0     281     565    1011 
   Size:        281     284     446

  Fragments arranged by size: 

                 446     284     281
 
BstBI TT'CG_AA

Cuts at:      0     773    1011 
   Size:        773     238
 
BstEII G'GTnAC_C

Cuts at:      0     588    1011 
   Size:        588     423
 
BstYI r'GATC_y

Cuts at:      0     100    1011 
   Size:        100     911
 
Bsu36I CC'TnA_GG

Cuts at:      0     373    1011 
   Size:        373     638
 
BtgI C'CryG_G

Cuts at:      0     644    1011 
   Size:        644     367
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     494    1011 
   Size:        494     517
 
DraI TTT'AAA

Cuts at:      0     842    1011 
   Size:        842     169
 
EarI CTCTTCn'nnn_

Cuts at:      0     163     658    1011 
   Size:        163     495     353

  Fragments arranged by size: 

                 495     353     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144    1011 
   Size:        144     867
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1002    1011 
   Size:       1002       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     231     263     377     409    1011 
   Size:        231      32     114      32     602

  Fragments arranged by size: 

                 602     231     114      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      67     283    1011 
   Size:         67     216     728

  Fragments arranged by size: 

                 728     216      67
 
NsiI A_TGCA'T

Cuts at:      0     192    1011 
   Size:        192     819
 
NspI r_CATG'y

Cuts at:      0      14     763    1011 
   Size:         14     749     248

  Fragments arranged by size: 

                 749     248      14
 
PciI A'CATG_T

Cuts at:      0      10    1011 
   Size:         10    1001
 
PpuMI rG'GwC_Cy

Cuts at:      0    1002    1011 
   Size:       1002       9
 
SmlI C'TyrA_G

Cuts at:      0    1006    1011 
   Size:       1006       5
 
SphI G_CATG'C

Cuts at:      0     763    1011 
   Size:        763     248
 
SspI AAT'ATT

Cuts at:      0      96     475    1011 
   Size:         96     379     536

  Fragments arranged by size: 

                 536     379      96
 
TatI w'GTAC_w

Cuts at:      0     281     565     866    1011 
   Size:        281     284     301     145

  Fragments arranged by size: 

                 301     284     281     145
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     417     787    1011 
   Size:        417     370     224

  Fragments arranged by size: 

                 417     370     224
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     ApoI     BbsI    BfrBI    BmgBI
     BmrI    BsaWI    BsaXI     BsgI     BsmI    BspMI    BsrDI    BsrGI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     DraI     EarI
  Eco57MI EcoO109I    Hin4I     MslI     NsiI     NspI     PciI    PpuMI
     SmlI     SphI     SspI     TatI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstXI  BstZ17I
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI