Restriction map for
pFN21AB7117
(Linear) (Six-base) MAPSORT of: KIBB7117.seq Check: 350 from: 1 to: 849
KIBB7117.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AatII G_ACGT'C
Cuts at: 0 509 849
Size: 509 340
AccI GT'mk_AC
Cuts at: 0 509 849
Size: 509 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 849
Size: 75 774
AleI CACnn'nnGTG
Cuts at: 0 393 849
Size: 393 456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 68 849
Size: 68 781
AvaI C'yCGr_G
Cuts at: 0 696 849
Size: 696 153
BanI G'GyrC_C
Cuts at: 0 802 849
Size: 802 47
BanII G_rGCy'C
Cuts at: 0 663 697 849
Size: 663 34 152
Fragments arranged by size:
663 152 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 435 558 567 849
Size: 435 123 9 282
Fragments arranged by size:
435 282 123 9
Bme1580I G_kGCm'C
Cuts at: 0 654 849
Size: 654 195
BmtI G_CTAG'C
Cuts at: 0 586 849
Size: 586 263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 849
Size: 88 761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 370 849
Size: 151 219 479
Fragments arranged by size:
479 219 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 849
Size: 343 506
BsaAI yAC'GTr
Cuts at: 0 282 849
Size: 282 567
BsaHI Gr'CG_yC
Cuts at: 0 506 849
Size: 506 343
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 367 849
Size: 256 111 482
Fragments arranged by size:
482 256 111
BseYI C'CCAG_C
Cuts at: 0 258 662 811 849
Size: 258 404 149 38
Fragments arranged by size:
404 258 149 38
BsiEI CG_ry'CG
Cuts at: 0 834 849
Size: 834 15
BsmI GAATG_Cn'
Cuts at: 0 45 849
Size: 45 804
Bsp1286I G_dGCh'C
Cuts at: 0 654 663 697 849
Size: 654 9 34 152
Fragments arranged by size:
654 152 34 9
BsrBI CCG'CTC
Cuts at: 0 671 849
Size: 671 178
BsrFI r'CCGG_y
Cuts at: 0 41 849
Size: 41 808
BsrGI T'GTAC_A
Cuts at: 0 17 849
Size: 17 832
BssSI C'ACGA_G
Cuts at: 0 268 849
Size: 268 581
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 528 849
Size: 282 246 321
Fragments arranged by size:
321 282 246
BtgI C'CryG_G
Cuts at: 0 330 482 849
Size: 330 152 367
Fragments arranged by size:
367 330 152
EaeI y'GGCC_r
Cuts at: 0 60 272 617 705 849
Size: 60 212 345 88 144
Fragments arranged by size:
345 212 144 88 60
EarI CTCTTCn'nnn_
Cuts at: 0 355 849
Size: 355 494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 151 370 849
Size: 75 76 219 479
Fragments arranged by size:
479 219 76 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 849
Size: 352 497
EcoO109I rG'GnC_Cy
Cuts at: 0 336 849
Size: 336 513
HaeII r_GCGC'y
Cuts at: 0 669 849
Size: 669 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 823 849
Size: 127 32 664 26
Fragments arranged by size:
664 127 32 26
MscI TGG'CCA
Cuts at: 0 274 849
Size: 274 575
MslI CAynn'nnrTG
Cuts at: 0 280 393 412 849
Size: 280 113 19 437
Fragments arranged by size:
437 280 113 19
MspA1I CmG'CkG
Cuts at: 0 836 849
Size: 836 13
NcoI C'CATG_G
Cuts at: 0 330 482 849
Size: 330 152 367
Fragments arranged by size:
367 330 152
NheI G'CTAG_C
Cuts at: 0 582 849
Size: 582 267
PmlI CAC'GTG
Cuts at: 0 282 849
Size: 282 567
PpuMI rG'GwC_Cy
Cuts at: 0 336 849
Size: 336 513
PshAI GACnn'nnGTC
Cuts at: 0 403 849
Size: 403 446
PstI C_TGCA'G
Cuts at: 0 120 239 849
Size: 120 119 610
Fragments arranged by size:
610 120 119
SanDI GG'GwC_CC
Cuts at: 0 336 849
Size: 336 513
SfcI C'TryA_G
Cuts at: 0 116 235 849
Size: 116 119 614
Fragments arranged by size:
614 119 116
SmlI C'TyrA_G
Cuts at: 0 322 849
Size: 322 527
StyI C'CwwG_G
Cuts at: 0 330 482 849
Size: 330 152 367
Fragments arranged by size:
367 330 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 66 849
Size: 66 783
TatI w'GTAC_w
Cuts at: 0 17 849
Size: 17 832
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 849
Size: 489 360
ZraI GAC'GTC
Cuts at: 0 507 849
Size: 507 342
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AvaI BanI BanII
BbsI Bme1580I BmtI BplI BpmI BpuEI BsaAI BsaHI
BseRI BseYI BsiEI BsmI Bsp1286I BsrBI BsrFI BsrGI
BssSI BstXI BtgI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I MscI MslI MspA1I NcoI NheI PmlI
PpuMI PshAI PstI SanDI SfcI SmlI StyI TaqII
TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI Bpu10I BsaI
BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BssHII BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI