Restriction map for
pFN21AB7063
(Linear) (Six-base) MAPSORT of: KIBB7063.seq Check: 9452 from: 1 to: 2019
KIBB7063.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AccI GT'mk_AC
Cuts at: 0 778 1246 2019
Size: 778 468 773
Fragments arranged by size:
778 773 468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 2019
Size: 1052 967
AflIII A'CryG_T
Cuts at: 0 1841 2019
Size: 1841 178
AhdI GACnn_n'nnGTC
Cuts at: 0 324 1872 2019
Size: 324 1548 147
Fragments arranged by size:
1548 324 147
AleI CACnn'nnGTG
Cuts at: 0 1495 2019
Size: 1495 524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 2019
Size: 46 32 1941
Fragments arranged by size:
1941 46 32
AlwNI CAG_nnn'CTG
Cuts at: 0 375 1416 2019
Size: 375 1041 603
Fragments arranged by size:
1041 603 375
ApaLI G'TGCA_C
Cuts at: 0 1083 2019
Size: 1083 936
ApoI r'AATT_y
Cuts at: 0 1509 1653 2019
Size: 1509 144 366
Fragments arranged by size:
1509 366 144
AvaI C'yCGr_G
Cuts at: 0 141 172 293 322 1260 2019
Size: 141 31 121 29 938 759
Fragments arranged by size:
938 759 141 121 31 29
BanI G'GyrC_C
Cuts at: 0 9 303 1120 2010 2019
Size: 9 294 817 890 9
Fragments arranged by size:
890 817 294 9 9
BanII G_rGCy'C
Cuts at: 0 71 119 130 268 430 517 694 1234
Size: 71 48 11 138 162 87 177 540
Cuts at: 1234 1261 1273 2019
Size: 27 12 746
Fragments arranged by size:
746 540 177 162 138 87 71 48
27 12 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 2019
Size: 197 1822
BbvCI CC'TCA_GC
Cuts at: 0 386 2019
Size: 386 1633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1284 1318 2019
Size: 1284 34 701
Fragments arranged by size:
1284 701 34
BclI T'GATC_A
Cuts at: 0 494 2019
Size: 494 1525
BglI GCCn_nnn'nGGC
Cuts at: 0 343 2019
Size: 343 1676
BglII A'GATC_T
Cuts at: 0 545 2019
Size: 545 1474
BlpI GC'TnA_GC
Cuts at: 0 355 1050 1985 2019
Size: 355 695 935 34
Fragments arranged by size:
935 695 355 34
Bme1580I G_kGCm'C
Cuts at: 0 575 1087 2019
Size: 575 512 932
Fragments arranged by size:
932 575 512
BmgBI CAC'GTC
Cuts at: 0 887 2019
Size: 887 1132
BmrI ACTGGGnnnn_n'
Cuts at: 0 1220 2019
Size: 1220 799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 2019
Size: 1393 626
Bpu10I CC'TnA_GC
Cuts at: 0 386 2019
Size: 386 1633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1695 2019
Size: 301 1394 324
Fragments arranged by size:
1394 324 301
BsaAI yAC'GTr
Cuts at: 0 95 2019
Size: 95 1924
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 838 868 2019
Size: 838 30 1151
Fragments arranged by size:
1151 838 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 193 1758 2019
Size: 190 3 1565 261
Fragments arranged by size:
1565 261 190 3
BseYI C'CCAG_C
Cuts at: 0 261 421 857 1196 1562 1822 2006 2019
Size: 261 160 436 339 366 260 184 13
Fragments arranged by size:
436 366 339 261 260 184 160 13
BsiEI CG_ry'CG
Cuts at: 0 155 236 2019
Size: 155 81 1783
Fragments arranged by size:
1783 155 81
BsiHKAI G_wGCw'C
Cuts at: 0 119 517 694 1087 1234 2019
Size: 119 398 177 393 147 785
Fragments arranged by size:
785 398 393 177 147 119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 142 2019
Size: 142 1877
Bsp1286I G_dGCh'C
Cuts at: 0 71 119 130 268 430 517 575 694
Size: 71 48 11 138 162 87 58 119
Cuts at: 694 1087 1234 1261 1273 2019
Size: 393 147 27 12 746
Fragments arranged by size:
746 393 162 147 138 119 87 71
58 48 27 12 11
BspHI T'CATG_A
Cuts at: 0 1451 2019
Size: 1451 568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1175 1424 1802 2019
Size: 1175 249 378 217
Fragments arranged by size:
1175 378 249 217
BsrBI CCG'CTC
Cuts at: 0 30 39 254 359 728 2019
Size: 30 9 215 105 369 1291
Fragments arranged by size:
1291 369 215 105 30 9
BsrDI GCAATG_nn'
Cuts at: 0 1542 2019
Size: 1542 477
BsrFI r'CCGG_y
Cuts at: 0 12 217 610 2019
Size: 12 205 393 1409
Fragments arranged by size:
1409 393 205 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 1082 2019
Size: 396 686 937
Fragments arranged by size:
937 686 396
BstEII G'GTnAC_C
Cuts at: 0 1396 2019
Size: 1396 623
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1830 2019
Size: 1830 189
BstYI r'GATC_y
Cuts at: 0 545 2019
Size: 545 1474
BstZ17I GTA'TAC
Cuts at: 0 779 1247 2019
Size: 779 468 772
Fragments arranged by size:
779 772 468
BtgI C'CryG_G
Cuts at: 0 227 341 758 789 1099 2019
Size: 227 114 417 31 310 920
Fragments arranged by size:
920 417 310 227 114 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1187 1460 2019
Size: 1187 273 559
Fragments arranged by size:
1187 559 273
BtsI GCAGTG_nn'
Cuts at: 0 643 903 2019
Size: 643 260 1116
Fragments arranged by size:
1116 643 260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 771 806 2019
Size: 771 35 1213
Fragments arranged by size:
1213 771 35
EaeI y'GGCC_r
Cuts at: 0 152 792 963 2019
Size: 152 640 171 1056
Fragments arranged by size:
1056 640 171 152
EagI C'GGCC_G
Cuts at: 0 152 2019
Size: 152 1867
EarI CTCTTCn'nnn_
Cuts at: 0 718 1383 2019
Size: 718 665 636
Fragments arranged by size:
718 665 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 474 2019
Size: 474 1545
EcoICRI GAG'CTC
Cuts at: 0 117 515 692 1232 2019
Size: 117 398 177 540 787
Fragments arranged by size:
787 540 398 177 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1393 2019
Size: 1052 341 626
Fragments arranged by size:
1052 626 341
EcoO109I rG'GnC_Cy
Cuts at: 0 1413 2019
Size: 1413 606
EcoRI G'AATT_C
Cuts at: 0 1509 1653 2019
Size: 1509 144 366
Fragments arranged by size:
1509 366 144
HaeII r_GCGC'y
Cuts at: 0 376 1628 2019
Size: 376 1252 391
Fragments arranged by size:
1252 391 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 838 870 1744 1776 1858 1890
Size: 310 32 496 32 874 32 82 32
Cuts at: 1890 2019
Size: 129
Fragments arranged by size:
874 496 310 129 82 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1445 1892 2019
Size: 977 468 447 127
Fragments arranged by size:
977 468 447 127
MslI CAynn'nnrTG
Cuts at: 0 1445 1495 2019
Size: 1445 50 524
Fragments arranged by size:
1445 524 50
MspA1I CmG'CkG
Cuts at: 0 343 396 841 1227 1495 2019
Size: 343 53 445 386 268 524
Fragments arranged by size:
524 445 386 343 268 53
NaeI GCC'GGC
Cuts at: 0 219 612 2019
Size: 219 393 1407
Fragments arranged by size:
1407 393 219
NcoI C'CATG_G
Cuts at: 0 227 758 2019
Size: 227 531 1261
Fragments arranged by size:
1261 531 227
NgoMIV G'CCGG_C
Cuts at: 0 217 610 2019
Size: 217 393 1409
Fragments arranged by size:
1409 393 217
NspI r_CATG'y
Cuts at: 0 1845 2019
Size: 1845 174
PasI CC'CwG_GG
Cuts at: 0 1620 2019
Size: 1620 399
PciI A'CATG_T
Cuts at: 0 1841 2019
Size: 1841 178
PfoI T'CCnGG_A
Cuts at: 0 1007 1409 2019
Size: 1007 402 610
Fragments arranged by size:
1007 610 402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 432 464 2019
Size: 432 32 1555
Fragments arranged by size:
1555 432 32
PpuMI rG'GwC_Cy
Cuts at: 0 1413 2019
Size: 1413 606
PshAI GACnn'nnGTC
Cuts at: 0 848 2019
Size: 848 1171
PstI C_TGCA'G
Cuts at: 0 383 824 2019
Size: 383 441 1195
Fragments arranged by size:
1195 441 383
PvuI CG_AT'CG
Cuts at: 0 236 2019
Size: 236 1783
PvuII CAG'CTG
Cuts at: 0 841 2019
Size: 841 1178
SacI G_AGCT'C
Cuts at: 0 119 517 694 1234 2019
Size: 119 398 177 540 785
Fragments arranged by size:
785 540 398 177 119
SacII CC_GC'GG
Cuts at: 0 344 2019
Size: 344 1675
SapI GCTCTTCn'nnn_
Cuts at: 0 718 2019
Size: 718 1301
SfcI C'TryA_G
Cuts at: 0 379 820 1041 2019
Size: 379 441 221 978
Fragments arranged by size:
978 441 379 221
SmaI CCC'GGG
Cuts at: 0 295 324 2019
Size: 295 29 1695
Fragments arranged by size:
1695 295 29
SmlI C'TyrA_G
Cuts at: 0 280 1674 2019
Size: 280 1394 345
Fragments arranged by size:
1394 345 280
SpeI A'CTAG_T
Cuts at: 0 1783 2019
Size: 1783 236
StuI AGG'CCT
Cuts at: 0 1993 2019
Size: 1993 26
StyI C'CwwG_G
Cuts at: 0 227 758 1253 1692 2019
Size: 227 531 495 439 327
Fragments arranged by size:
531 495 439 327 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1998 2019
Size: 1998 21
TatI w'GTAC_w
Cuts at: 0 1775 2019
Size: 1775 244
Tth111I GACn'n_nGTC
Cuts at: 0 1461 2019
Size: 1461 558
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1627 2019
Size: 1627 392
XmaI C'CCGG_G
Cuts at: 0 293 322 2019
Size: 293 29 1697
Fragments arranged by size:
1697 293 29
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AloI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII
Hin4I MmeI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PfoI PpiI PpuMI PshAI PstI PvuI
PvuII SacI SacII SapI SfcI SmaI SmlI SpeI
StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BfrBI BmtI BplI BsaI BsaBI BsaHI BsaWI
BsgI BsiWI BsmI BspEI BsrGI BssHII BssSI BstBI
Bsu36I ClaI DraI DraIII DrdI EcoNI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PsiI PspOMI PspXI
PsrI RsrII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SphI SrfI SspI SwaI
TstI XbaI XhoI XmnI ZraI