Restriction map for
pFN21AB7056
(Linear) (Six-base) MAPSORT of: KIBB7056.seq Check: 6207 from: 1 to: 1752
KIBB7056.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1448 1752
Size: 1448 304
AatII G_ACGT'C
Cuts at: 0 1091 1752
Size: 1091 661
AccI GT'mk_AC
Cuts at: 0 1091 1752
Size: 1091 661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1050 1078 1294 1378 1752
Size: 1050 28 216 84 374
Fragments arranged by size:
1050 374 216 84 28
AflIII A'CryG_T
Cuts at: 0 218 362 1752
Size: 218 144 1390
Fragments arranged by size:
1390 218 144
AhdI GACnn_n'nnGTC
Cuts at: 0 1017 1752
Size: 1017 735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 973 1752
Size: 973 779
AlwNI CAG_nnn'CTG
Cuts at: 0 93 393 1412 1752
Size: 93 300 1019 340
Fragments arranged by size:
1019 340 300 93
ApaI G_GGCC'C
Cuts at: 0 670 926 1752
Size: 670 256 826
Fragments arranged by size:
826 670 256
ApaLI G'TGCA_C
Cuts at: 0 1279 1375 1752
Size: 1279 96 377
Fragments arranged by size:
1279 377 96
AvaI C'yCGr_G
Cuts at: 0 481 720 862 1533 1711 1752
Size: 481 239 142 671 178 41
Fragments arranged by size:
671 481 239 178 142 41
BamHI G'GATC_C
Cuts at: 0 29 1752
Size: 29 1723
BanI G'GyrC_C
Cuts at: 0 196 409 1072 1177 1330 1518 1555 1678
Size: 196 213 663 105 153 188 37 123
Cuts at: 1678 1752
Size: 74
Fragments arranged by size:
663 213 196 188 153 123 105 74
37
BanII G_rGCy'C
Cuts at: 0 670 926 1752
Size: 670 256 826
Fragments arranged by size:
826 670 256
BbeI G_GCGC'C
Cuts at: 0 200 413 1076 1181 1334 1522 1559 1682
Size: 200 213 663 105 153 188 37 123
Cuts at: 1682 1752
Size: 70
Fragments arranged by size:
663 213 200 188 153 123 105 70
37
BbvCI CC'TCA_GC
Cuts at: 0 322 1752
Size: 322 1430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1404 1438 1752
Size: 1404 34 314
Fragments arranged by size:
1404 314 34
BclI T'GATC_A
Cuts at: 0 447 1752
Size: 447 1305
BglI GCCn_nnn'nGGC
Cuts at: 0 280 565 616 625 790 815 1652 1752
Size: 280 285 51 9 165 25 837 100
Fragments arranged by size:
837 285 280 165 100 51 25 9
Bme1580I G_kGCm'C
Cuts at: 0 670 926 1153 1283 1379 1752
Size: 670 256 227 130 96 373
Fragments arranged by size:
670 373 256 227 130 96
BmgBI CAC'GTC
Cuts at: 0 105 191 930 1638 1752
Size: 105 86 739 708 114
Fragments arranged by size:
739 708 114 105 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 1752
Size: 858 894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1752
Size: 361 1391
Bpu10I CC'TnA_GC
Cuts at: 0 322 1752
Size: 322 1430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1438 1752
Size: 1438 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 769 1752
Size: 769 983
BsaAI yAC'GTr
Cuts at: 0 363 1752
Size: 363 1389
BsaHI Gr'CG_yC
Cuts at: 0 197 410 416 568 749 1040 1073 1088
Size: 197 213 6 152 181 291 33 15
Cuts at: 1088 1178 1331 1475 1519 1556 1583 1679 1752
Size: 90 153 144 44 37 27 96 73
Fragments arranged by size:
291 213 197 181 153 152 144 96
90 73 44 37 33 27 15 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 844 847 1030 1703 1752
Size: 451 393 3 183 673 49
Fragments arranged by size:
673 451 393 183 49 3
BseYI C'CCAG_C
Cuts at: 0 1528 1565 1570 1752
Size: 1528 37 5 182
Fragments arranged by size:
1528 182 37 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1426 1752
Size: 1426 326
BsiEI CG_ry'CG
Cuts at: 0 401 527 677 794 819 902 1494 1752
Size: 401 126 150 117 25 83 592 258
Fragments arranged by size:
592 401 258 150 126 117 83 25
BsiHKAI G_wGCw'C
Cuts at: 0 1283 1379 1752
Size: 1283 96 373
Fragments arranged by size:
1283 373 96
Bsp1286I G_dGCh'C
Cuts at: 0 670 926 1153 1283 1379 1752
Size: 670 256 227 130 96 373
Fragments arranged by size:
670 373 256 227 130 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1448 1752
Size: 1448 304
BsrBI CCG'CTC
Cuts at: 0 146 681 738 898 1430 1752
Size: 146 535 57 160 532 322
Fragments arranged by size:
535 532 322 160 146 57
BsrFI r'CCGG_y
Cuts at: 0 4 383 777 789 1180 1661 1681 1752
Size: 4 379 394 12 391 481 20 71
Fragments arranged by size:
481 394 391 379 71 20 12 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 1241 1752
Size: 93 1148 511
Fragments arranged by size:
1148 511 93
BstYI r'GATC_y
Cuts at: 0 29 344 389 1124 1752
Size: 29 315 45 735 628
Fragments arranged by size:
735 628 315 45 29
BtgI C'CryG_G
Cuts at: 0 200 521 550 602 623 629 819 995
Size: 200 321 29 52 21 6 190 176
Cuts at: 995 1488 1752
Size: 493 264
Fragments arranged by size:
493 321 264 200 190 176 52 29
21 6
BtsI GCAGTG_nn'
Cuts at: 0 1330 1752
Size: 1330 422
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1087 1752
Size: 1039 48 665
Fragments arranged by size:
1039 665 48
EaeI y'GGCC_r
Cuts at: 0 2 155 281 398 524 605 617 626
Size: 2 153 126 117 126 81 12 9
Cuts at: 626 674 730 791 807 816 899 944 1491
Size: 48 56 61 16 9 83 45 547
Cuts at: 1491 1596 1640 1752
Size: 105 44 112
Fragments arranged by size:
547 153 126 126 117 112 105 83
81 61 56 48 45 44 16 12
9 9 2
EagI C'GGCC_G
Cuts at: 0 398 524 674 791 816 899 1491 1752
Size: 398 126 150 117 25 83 592 261
Fragments arranged by size:
592 398 261 150 126 117 83 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 546 761 839 1752
Size: 546 215 78 913
Fragments arranged by size:
913 546 215 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1050 1078 1294 1378 1752
Size: 361 689 28 216 84 374
Fragments arranged by size:
689 374 361 216 84 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 1752
Size: 118 1634
EcoO109I rG'GnC_Cy
Cuts at: 0 90 667 1752
Size: 90 577 1085
Fragments arranged by size:
1085 577 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1269 1752
Size: 1269 483
HaeII r_GCGC'y
Cuts at: 0 200 245 253 329 413 1076 1181 1334
Size: 200 45 8 76 84 663 105 153
Cuts at: 1334 1522 1559 1682 1752
Size: 188 37 123 70
Fragments arranged by size:
663 200 188 153 123 105 84 76
70 45 37 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1003 1035 1752
Size: 1003 32 717
Fragments arranged by size:
1003 717 32
KasI G'GCGC_C
Cuts at: 0 196 409 1072 1177 1330 1518 1555 1678
Size: 196 213 663 105 153 188 37 123
Cuts at: 1678 1752
Size: 74
Fragments arranged by size:
663 213 196 188 153 123 105 74
37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1457 1752
Size: 830 627 295
Fragments arranged by size:
830 627 295
MscI TGG'CCA
Cuts at: 0 732 1642 1752
Size: 732 910 110
Fragments arranged by size:
910 732 110
MslI CAynn'nnrTG
Cuts at: 0 1372 1752
Size: 1372 380
MspA1I CmG'CkG
Cuts at: 0 81 426 523 538 613 631 821 997
Size: 81 345 97 15 75 18 190 176
Cuts at: 997 1070 1409 1490 1700 1752
Size: 73 339 81 210 52
Fragments arranged by size:
345 339 210 190 176 97 81 81
75 73 52 18 15
NaeI GCC'GGC
Cuts at: 0 6 791 1182 1752
Size: 6 785 391 570
Fragments arranged by size:
785 570 391 6
NarI GG'CG_CC
Cuts at: 0 197 410 1073 1178 1331 1519 1556 1679
Size: 197 213 663 105 153 188 37 123
Cuts at: 1679 1752
Size: 73
Fragments arranged by size:
663 213 197 188 153 123 105 73
37
NgoMIV G'CCGG_C
Cuts at: 0 4 789 1180 1752
Size: 4 785 391 572
Fragments arranged by size:
785 572 391 4
NotI GC'GGCC_GC
Cuts at: 0 524 1491 1752
Size: 524 967 261
Fragments arranged by size:
967 524 261
NspI r_CATG'y
Cuts at: 0 1287 1371 1752
Size: 1287 84 381
Fragments arranged by size:
1287 381 84
PfoI T'CCnGG_A
Cuts at: 0 377 1752
Size: 377 1375
PmlI CAC'GTG
Cuts at: 0 363 1752
Size: 363 1389
PshAI GACnn'nnGTC
Cuts at: 0 1423 1752
Size: 1423 329
PspOMI G'GGCC_C
Cuts at: 0 666 922 1752
Size: 666 256 830
Fragments arranged by size:
830 666 256
PspXI vC'TCGA_Gb
Cuts at: 0 481 1752
Size: 481 1271
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 469 501 796 828 1752
Size: 469 32 295 32 924
Fragments arranged by size:
924 469 295 32 32
PstI C_TGCA'G
Cuts at: 0 166 962 1752
Size: 166 796 790
Fragments arranged by size:
796 790 166
PvuII CAG'CTG
Cuts at: 0 426 1409 1752
Size: 426 983 343
Fragments arranged by size:
983 426 343
SacII CC_GC'GG
Cuts at: 0 524 632 822 998 1491 1752
Size: 524 108 190 176 493 261
Fragments arranged by size:
524 493 261 190 176 108
ScaI AGT'ACT
Cuts at: 0 172 1063 1752
Size: 172 891 689
Fragments arranged by size:
891 689 172
SfcI C'TryA_G
Cuts at: 0 162 958 1752
Size: 162 796 794
Fragments arranged by size:
796 794 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 625 790 815 1752
Size: 625 165 25 937
Fragments arranged by size:
937 625 165 25
SfoI GGC'GCC
Cuts at: 0 198 411 1074 1179 1332 1520 1557 1680
Size: 198 213 663 105 153 188 37 123
Cuts at: 1680 1752
Size: 72
Fragments arranged by size:
663 213 198 188 153 123 105 72
37
SgrAI Cr'CCGG_yG
Cuts at: 0 777 1681 1752
Size: 777 904 71
Fragments arranged by size:
904 777 71
SmaI CCC'GGG
Cuts at: 0 722 1535 1713 1752
Size: 722 813 178 39
Fragments arranged by size:
813 722 178 39
SmlI C'TyrA_G
Cuts at: 0 481 1453 1752
Size: 481 972 299
Fragments arranged by size:
972 481 299
StuI AGG'CCT
Cuts at: 0 1138 1752
Size: 1138 614
StyI C'CwwG_G
Cuts at: 0 470 593 1133 1752
Size: 470 123 540 619
Fragments arranged by size:
619 540 470 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 1222 1752
Size: 301 921 530
Fragments arranged by size:
921 530 301
TatI w'GTAC_w
Cuts at: 0 170 1061 1752
Size: 170 891 691
Fragments arranged by size:
891 691 170
Tth111I GACn'n_nGTC
Cuts at: 0 235 1752
Size: 235 1517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1752
Size: 600 1152
XhoI C'TCGA_G
Cuts at: 0 481 1752
Size: 481 1271
XmaI C'CCGG_G
Cuts at: 0 720 1533 1711 1752
Size: 720 813 178 41
Fragments arranged by size:
813 720 178 41
XmnI GAAnn'nnTTC
Cuts at: 0 184 1063 1752
Size: 184 879 689
Fragments arranged by size:
879 689 184
ZraI GAC'GTC
Cuts at: 0 1089 1752
Size: 1089 663
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BclI BglI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI BtgI
BtsI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PfoI PmlI PshAI
PspOMI PspXI PsrI PstI PvuII SacII ScaI SfcI
SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BciVI BfrBI
BglII BlpI BmrI BmtI BsaBI BsaWI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EarI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PpuMI PsiI PvuI RsrII SacI SalI
SanDI SapI SbfI SexAI SgfI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI