Restriction map for
pFN21AB7053
(Linear) (Six-base) MAPSORT of: KIBB7053.seq Check: 9857 from: 1 to: 1629
KIBB7053.orf
With 182 enzymes: SgfI *
April 19, 2010 16:26 ..
AccI GT'mk_AC
Cuts at: 0 868 1629
Size: 868 761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 931 1113 1548 1629
Size: 447 484 182 435 81
Fragments arranged by size:
484 447 435 182 81
AfeI AGC'GCT
Cuts at: 0 695 1629
Size: 695 934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1135 1629
Size: 1135 494
AlwNI CAG_nnn'CTG
Cuts at: 0 828 909 1629
Size: 828 81 720
Fragments arranged by size:
828 720 81
ApaI G_GGCC'C
Cuts at: 0 162 1629
Size: 162 1467
ApaLI G'TGCA_C
Cuts at: 0 1456 1629
Size: 1456 173
ApoI r'AATT_y
Cuts at: 0 1622 1629
Size: 1622 7
AvaI C'yCGr_G
Cuts at: 0 451 675 1629
Size: 451 224 954
Fragments arranged by size:
954 451 224
BamHI G'GATC_C
Cuts at: 0 61 1629
Size: 61 1568
BanI G'GyrC_C
Cuts at: 0 148 615 799 1629
Size: 148 467 184 830
Fragments arranged by size:
830 467 184 148
BanII G_rGCy'C
Cuts at: 0 135 162 458 562 682 901 1064 1629
Size: 135 27 296 104 120 219 163 565
Fragments arranged by size:
565 296 219 163 135 120 104 27
BbeI G_GCGC'C
Cuts at: 0 152 1629
Size: 152 1477
BbvCI CC'TCA_GC
Cuts at: 0 516 1545 1629
Size: 516 1029 84
Fragments arranged by size:
1029 516 84
BglI GCCn_nnn'nGGC
Cuts at: 0 157 1300 1629
Size: 157 1143 329
Fragments arranged by size:
1143 329 157
BglII A'GATC_T
Cuts at: 0 44 1629
Size: 44 1585
BlpI GC'TnA_GC
Cuts at: 0 121 1629
Size: 121 1508
Bme1580I G_kGCm'C
Cuts at: 0 162 802 1412 1460 1629
Size: 162 640 610 48 169
Fragments arranged by size:
640 610 169 162 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 1015 1629
Size: 132 883 614
Fragments arranged by size:
883 614 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 436 512 682 850 1629
Size: 124 312 76 170 168 779
Fragments arranged by size:
779 312 170 168 124 76
Bpu10I CC'TnA_GC
Cuts at: 0 98 516 568 653 1545 1629
Size: 98 418 52 85 892 84
Fragments arranged by size:
892 418 98 85 84 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 1629
Size: 346 1283
BsaAI yAC'GTr
Cuts at: 0 1493 1629
Size: 1493 136
BsaHI Gr'CG_yC
Cuts at: 0 149 991 1629
Size: 149 842 638
Fragments arranged by size:
842 638 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1590 1620 1629
Size: 1590 30 9
Fragments arranged by size:
1590 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 498 528 1299 1413 1425 1488 1629
Size: 121 377 30 771 114 12 63 141
Fragments arranged by size:
771 377 141 121 114 63 30 12
BseYI C'CCAG_C
Cuts at: 0 364 842 938 952 982 1629
Size: 364 478 96 14 30 647
Fragments arranged by size:
647 478 364 96 30 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 1629
Size: 407 1222
BsiHKAI G_wGCw'C
Cuts at: 0 296 1064 1460 1629
Size: 296 768 396 169
Fragments arranged by size:
768 396 296 169
Bsp1286I G_dGCh'C
Cuts at: 0 135 162 296 458 562 682 802 901
Size: 135 27 134 162 104 120 120 99
Cuts at: 901 1064 1412 1460 1629
Size: 163 348 48 169
Fragments arranged by size:
348 169 163 162 135 134 120 120
104 99 48 27
BsrBI CCG'CTC
Cuts at: 0 1052 1629
Size: 1052 577
BsrFI r'CCGG_y
Cuts at: 0 501 977 1629
Size: 501 476 652
Fragments arranged by size:
652 501 476
BstYI r'GATC_y
Cuts at: 0 44 61 786 1247 1629
Size: 44 17 725 461 382
Fragments arranged by size:
725 461 382 44 17
Bsu36I CC'TnA_GG
Cuts at: 0 263 1629
Size: 263 1366
BtgI C'CryG_G
Cuts at: 0 5 83 1629
Size: 5 78 1546
Fragments arranged by size:
1546 78 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1238 1629
Size: 1238 391
BtsI GCAGTG_nn'
Cuts at: 0 584 1629
Size: 584 1045
DraIII CAC_nnn'GTG
Cuts at: 0 446 1629
Size: 446 1183
DrdI GACnn_nn'nnGTC
Cuts at: 0 306 1629
Size: 306 1323
EaeI y'GGCC_r
Cuts at: 0 2 8 402 1301 1382 1484 1629
Size: 2 6 394 899 81 102 145
Fragments arranged by size:
899 394 145 102 81 6 2
EarI CTCTTCn'nnn_
Cuts at: 0 466 1302 1323 1629
Size: 466 836 21 306
Fragments arranged by size:
836 466 306 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 531 537 1629
Size: 283 248 6 1092
Fragments arranged by size:
1092 283 248 6
EcoICRI GAG'CTC
Cuts at: 0 1062 1629
Size: 1062 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 436 447 512 682 850 931 1113
Size: 124 312 11 65 170 168 81 182
Cuts at: 1113 1548 1629
Size: 435 81
Fragments arranged by size:
435 312 182 170 168 124 81 81
65 11
EcoO109I rG'GnC_Cy
Cuts at: 0 158 159 825 906 915 1629
Size: 158 1 666 81 9 714
Fragments arranged by size:
714 666 158 81 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 1269 1629
Size: 753 516 360
Fragments arranged by size:
753 516 360
FspI TGC'GCA
Cuts at: 0 576 964 1123 1629
Size: 576 388 159 506
Fragments arranged by size:
576 506 388 159
HaeII r_GCGC'y
Cuts at: 0 152 697 1571 1629
Size: 152 545 874 58
Fragments arranged by size:
874 545 152 58
KasI G'GCGC_C
Cuts at: 0 148 1629
Size: 148 1481
MfeI C'AATT_G
Cuts at: 0 1616 1629
Size: 1616 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1079 1629
Size: 611 468 550
Fragments arranged by size:
611 550 468
MscI TGG'CCA
Cuts at: 0 1486 1629
Size: 1486 143
MslI CAynn'nnrTG
Cuts at: 0 1117 1629
Size: 1117 512
MspA1I CmG'CkG
Cuts at: 0 7 27 39 173 200 532 613 1549
Size: 7 20 12 134 27 332 81 936
Cuts at: 1549 1629
Size: 80
Fragments arranged by size:
936 332 134 81 80 27 20 12
7
NarI GG'CG_CC
Cuts at: 0 149 1629
Size: 149 1480
NcoI C'CATG_G
Cuts at: 0 83 1629
Size: 83 1546
NspI r_CATG'y
Cuts at: 0 963 1122 1629
Size: 963 159 507
Fragments arranged by size:
963 507 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1629
Size: 828 801
PpuMI rG'GwC_Cy
Cuts at: 0 906 1629
Size: 906 723
PshAI GACnn'nnGTC
Cuts at: 0 596 1629
Size: 596 1033
PspOMI G'GGCC_C
Cuts at: 0 158 1629
Size: 158 1471
PstI C_TGCA'G
Cuts at: 0 44 1629
Size: 44 1585
PvuII CAG'CTG
Cuts at: 0 27 1549 1629
Size: 27 1522 80
Fragments arranged by size:
1522 80 27
SacI G_AGCT'C
Cuts at: 0 1064 1629
Size: 1064 565
SacII CC_GC'GG
Cuts at: 0 8 1629
Size: 8 1621
SfcI C'TryA_G
Cuts at: 0 40 1629
Size: 40 1589
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1300 1629
Size: 1300 329
SfoI GGC'GCC
Cuts at: 0 150 1629
Size: 150 1479
SmaI CCC'GGG
Cuts at: 0 677 1629
Size: 677 952
SphI G_CATG'C
Cuts at: 0 963 1122 1629
Size: 963 159 507
Fragments arranged by size:
963 507 159
StuI AGG'CCT
Cuts at: 0 670 880 1629
Size: 670 210 749
Fragments arranged by size:
749 670 210
StyI C'CwwG_G
Cuts at: 0 11 83 728 1629
Size: 11 72 645 901
Fragments arranged by size:
901 645 72 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 441 633 856 1168 1180 1629
Size: 441 192 223 312 12 449
Fragments arranged by size:
449 441 312 223 192 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 608 640 1629
Size: 608 32 989
Fragments arranged by size:
989 608 32
XmaI C'CCGG_G
Cuts at: 0 675 1629
Size: 675 954
XmnI GAAnn'nnTTC
Cuts at: 0 817 1099 1629
Size: 817 282 530
Fragments arranged by size:
817 530 282
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbvCI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NspI PflMI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SfcI SfiI SfoI SmaI SphI StuI StyI
TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI EagI EcoNI EcoRI
EcoRV FseI FspAI Hin4I HincII HindIII HpaI KpnI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XcmI XhoI ZraI