Restriction map for
pFN21AB7044
(Linear) (Six-base) MAPSORT of: KIBB7044.seq Check: 3104 from: 1 to: 1416
KIBB7044.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 169 201 1416
Size: 169 32 1215
Fragments arranged by size:
1215 169 32
Acc65I G'GTAC_C
Cuts at: 0 1100 1416
Size: 1100 316
AclI AA'CG_TT
Cuts at: 0 1043 1416
Size: 1043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 492 711 1239 1416
Size: 482 10 219 528 177
Fragments arranged by size:
528 482 219 177 10
AflIII A'CryG_T
Cuts at: 0 1013 1416
Size: 1013 403
AhdI GACnn_n'nnGTC
Cuts at: 0 483 1374 1416
Size: 483 891 42
Fragments arranged by size:
891 483 42
AlwNI CAG_nnn'CTG
Cuts at: 0 904 1416
Size: 904 512
ApaI G_GGCC'C
Cuts at: 0 290 703 1416
Size: 290 413 713
Fragments arranged by size:
713 413 290
AvaI C'yCGr_G
Cuts at: 0 421 640 705 1416
Size: 421 219 65 711
Fragments arranged by size:
711 421 219 65
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1084 1117 1416
Size: 1084 33 299
Fragments arranged by size:
1084 299 33
BanI G'GyrC_C
Cuts at: 0 60 1100 1416
Size: 60 1040 316
Fragments arranged by size:
1040 316 60
BanII G_rGCy'C
Cuts at: 0 290 429 583 703 806 958 1147 1416
Size: 290 139 154 120 103 152 189 269
Fragments arranged by size:
290 269 189 154 152 139 120 103
BbeI G_GCGC'C
Cuts at: 0 64 1416
Size: 64 1352
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 965 1245 1298 1416
Size: 498 467 280 53 118
Fragments arranged by size:
498 467 280 118 53
BbvCI CC'TCA_GC
Cuts at: 0 1114 1207 1416
Size: 1114 93 209
Fragments arranged by size:
1114 209 93
BciVI GTATCCnnnnn_n'
Cuts at: 0 604 1416
Size: 604 812
BglI GCCn_nnn'nGGC
Cuts at: 0 379 1416
Size: 379 1037
Bme1580I G_kGCm'C
Cuts at: 0 290 703 935 1416
Size: 290 413 232 481
Fragments arranged by size:
481 413 290 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 1046 1209 1229 1416
Size: 1046 163 20 187
Fragments arranged by size:
1046 187 163 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 720 1416
Size: 720 696
Bpu10I CC'TnA_GC
Cuts at: 0 1114 1207 1416
Size: 1114 93 209
Fragments arranged by size:
1114 209 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 796 820 972 1416
Size: 106 690 24 152 444
Fragments arranged by size:
690 444 152 106 24
BsaBI GATnn'nnATC
Cuts at: 0 1306 1416
Size: 1306 110
BsaHI Gr'CG_yC
Cuts at: 0 61 107 305 1416
Size: 61 46 198 1111
Fragments arranged by size:
1111 198 61 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 546 1374 1416
Size: 546 828 42
Fragments arranged by size:
828 546 42
BseYI C'CCAG_C
Cuts at: 0 1063 1088 1416
Size: 1063 25 328
Fragments arranged by size:
1063 328 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1416
Size: 141 1275
BsiEI CG_ry'CG
Cuts at: 0 389 1416
Size: 389 1027
BsiHKAI G_wGCw'C
Cuts at: 0 806 958 1416
Size: 806 152 458
Fragments arranged by size:
806 458 152
BsmBI CGTCTCn'nnnn_
Cuts at: 0 470 1416
Size: 470 946
Bsp1286I G_dGCh'C
Cuts at: 0 290 429 583 703 806 935 958 1147
Size: 290 139 154 120 103 129 23 189
Cuts at: 1147 1416
Size: 269
Fragments arranged by size:
290 269 189 154 139 129 120 103
23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 201 1416
Size: 169 32 1215
Fragments arranged by size:
1215 169 32
BsrBI CCG'CTC
Cuts at: 0 189 1416
Size: 189 1227
BsrFI r'CCGG_y
Cuts at: 0 224 1416
Size: 224 1192
BsrGI T'GTAC_A
Cuts at: 0 26 1416
Size: 26 1390
BssHII G'CGCG_C
Cuts at: 0 86 88 273 1416
Size: 86 2 185 1143
Fragments arranged by size:
1143 185 86 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1059 1416
Size: 1059 357
BtgI C'CryG_G
Cuts at: 0 55 102 383 596 842 1286 1416
Size: 55 47 281 213 246 444 130
Fragments arranged by size:
444 281 246 213 130 55 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 801 836 1416
Size: 801 35 580
Fragments arranged by size:
801 580 35
DraIII CAC_nnn'GTG
Cuts at: 0 1172 1416
Size: 1172 244
EaeI y'GGCC_r
Cuts at: 0 380 386 1416
Size: 380 6 1030
Fragments arranged by size:
1030 380 6
EagI C'GGCC_G
Cuts at: 0 386 1416
Size: 386 1030
EcoICRI GAG'CTC
Cuts at: 0 804 956 1416
Size: 804 152 460
Fragments arranged by size:
804 460 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 492 711 1239 1416
Size: 482 10 219 528 177
Fragments arranged by size:
528 482 219 177 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 694 1416
Size: 694 722
EcoO109I rG'GnC_Cy
Cuts at: 0 286 287 699 700 828 1211 1416
Size: 286 1 412 1 128 383 205
Fragments arranged by size:
412 383 286 205 128 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 487 582 1416
Size: 487 95 834
Fragments arranged by size:
834 487 95
FspI TGC'GCA
Cuts at: 0 209 1416
Size: 209 1207
HaeII r_GCGC'y
Cuts at: 0 64 220 842 1416
Size: 64 156 622 574
Fragments arranged by size:
622 574 156 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 577 602 609 634 702 734
Size: 469 32 76 25 7 25 68 32
Cuts at: 734 857 889 1360 1392 1416
Size: 123 32 471 32 24
Fragments arranged by size:
471 469 123 76 68 32 32 32
32 25 25 24 7
KasI G'GCGC_C
Cuts at: 0 60 1416
Size: 60 1356
KpnI G_GTAC'C
Cuts at: 0 1104 1416
Size: 1104 312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1416
Size: 382 1034
MspA1I CmG'CkG
Cuts at: 0 195 385 1107 1206 1416
Size: 195 190 722 99 210
Fragments arranged by size:
722 210 195 190 99
NaeI GCC'GGC
Cuts at: 0 226 1416
Size: 226 1190
NarI GG'CG_CC
Cuts at: 0 61 1416
Size: 61 1355
NcoI C'CATG_G
Cuts at: 0 102 1416
Size: 102 1314
NgoMIV G'CCGG_C
Cuts at: 0 224 1416
Size: 224 1192
NotI GC'GGCC_GC
Cuts at: 0 386 1416
Size: 386 1030
PpuMI rG'GwC_Cy
Cuts at: 0 828 1211 1416
Size: 828 383 205
Fragments arranged by size:
828 383 205
PspOMI G'GGCC_C
Cuts at: 0 286 699 1416
Size: 286 413 717
Fragments arranged by size:
717 413 286
PstI C_TGCA'G
Cuts at: 0 71 434 1076 1193 1416
Size: 71 363 642 117 223
Fragments arranged by size:
642 363 223 117 71
PvuII CAG'CTG
Cuts at: 0 1206 1416
Size: 1206 210
SacI G_AGCT'C
Cuts at: 0 806 958 1416
Size: 806 152 458
Fragments arranged by size:
806 458 152
SacII CC_GC'GG
Cuts at: 0 386 1416
Size: 386 1030
SexAI A'CCwGG_T
Cuts at: 0 830 1416
Size: 830 586
SfcI C'TryA_G
Cuts at: 0 67 430 1072 1189 1416
Size: 67 363 642 117 227
Fragments arranged by size:
642 363 227 117 67
SfoI GGC'GCC
Cuts at: 0 62 1416
Size: 62 1354
SmaI CCC'GGG
Cuts at: 0 423 1416
Size: 423 993
SmlI C'TyrA_G
Cuts at: 0 121 799 811 951 1416
Size: 121 678 12 140 465
Fragments arranged by size:
678 465 140 121 12
StyI C'CwwG_G
Cuts at: 0 102 1058 1416
Size: 102 956 358
Fragments arranged by size:
956 358 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1310 1416
Size: 1310 106
TatI w'GTAC_w
Cuts at: 0 26 1416
Size: 26 1390
Tth111I GACn'n_nGTC
Cuts at: 0 307 1416
Size: 307 1109
XbaI T'CTAG_A
Cuts at: 0 1393 1416
Size: 1393 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 984 1416
Size: 243 741 432
Fragments arranged by size:
741 432 243
XmaI C'CCGG_G
Cuts at: 0 421 1416
Size: 421 995
XmnI GAAnn'nnTTC
Cuts at: 0 851 1416
Size: 851 565
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AhdI AlwNI ApaI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BciVI
BglI Bme1580I BmrI BplI Bpu10I BpuEI BsaBI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BstXI BtgI CspCI DraIII
EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI KpnI MmeI MspA1I NaeI NarI
NcoI NgoMIV NotI PpuMI PspOMI PstI PvuII SacI
SacII SexAI SfcI SfoI SmaI SmlI StyI TaqII
TatI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BcgI
BclI BfrBI BglII BlpI BmgBI BmtI BpmI BsaI
BsaAI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI DraI DrdI EarI EciI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI MfeI MluI MscI
MslI NdeI NheI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XhoI ZraI