Restriction map for
pFN21AB6995
(Linear) (Six-base) MAPSORT of: KIBB6995.seq Check: 408 from: 1 to: 666
KIBB6995.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 666
Size: 328 338
AflIII A'CryG_T
Cuts at: 0 262 666
Size: 262 404
AhdI GACnn_n'nnGTC
Cuts at: 0 353 666
Size: 353 313
AlwNI CAG_nnn'CTG
Cuts at: 0 43 536 666
Size: 43 493 130
Fragments arranged by size:
493 130 43
AseI AT'TA_AT
Cuts at: 0 422 666
Size: 422 244
AvaI C'yCGr_G
Cuts at: 0 495 605 666
Size: 495 110 61
Fragments arranged by size:
495 110 61
BanI G'GyrC_C
Cuts at: 0 308 666
Size: 308 358
BanII G_rGCy'C
Cuts at: 0 184 630 666
Size: 184 446 36
Fragments arranged by size:
446 184 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 334 648 666
Size: 334 314 18
Fragments arranged by size:
334 314 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 614 648 666
Size: 614 34 18
Fragments arranged by size:
614 34 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 666
Size: 561 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 246 666
Size: 198 48 420
Fragments arranged by size:
420 198 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 666
Size: 181 485
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 136 666
Size: 73 63 530
Fragments arranged by size:
530 73 63
BsaAI yAC'GTr
Cuts at: 0 263 666
Size: 263 403
BsaHI Gr'CG_yC
Cuts at: 0 621 666
Size: 621 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 154 184 187 623 666
Size: 130 24 30 3 436 43
Fragments arranged by size:
436 130 43 30 24 3
Bsp1286I G_dGCh'C
Cuts at: 0 184 630 666
Size: 184 446 36
Fragments arranged by size:
446 184 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 325 666
Size: 325 341
BssHII G'CGCG_C
Cuts at: 0 449 666
Size: 449 217
BstAPI GCAn_nnn'nTGC
Cuts at: 0 43 666
Size: 43 623
Bsu36I CC'TnA_GG
Cuts at: 0 107 269 666
Size: 107 162 397
Fragments arranged by size:
397 162 107
BtgI C'CryG_G
Cuts at: 0 576 666
Size: 576 90
BtsI GCAGTG_nn'
Cuts at: 0 585 630 666
Size: 585 45 36
Fragments arranged by size:
585 45 36
DraIII CAC_nnn'GTG
Cuts at: 0 591 666
Size: 591 75
EarI CTCTTCn'nnn_
Cuts at: 0 29 53 161 269 666
Size: 29 24 108 108 397
Fragments arranged by size:
397 108 108 29 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 328 666
Size: 181 147 338
Fragments arranged by size:
338 181 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 665 666
Size: 665 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 470 502 666
Size: 339 32 99 32 164
Fragments arranged by size:
339 164 99 32 32
HincII GTy'rAC
Cuts at: 0 525 666
Size: 525 141
HindIII A'AGCT_T
Cuts at: 0 359 666
Size: 359 307
MspA1I CmG'CkG
Cuts at: 0 536 578 666
Size: 536 42 88
Fragments arranged by size:
536 88 42
PasI CC'CwG_GG
Cuts at: 0 304 666
Size: 304 362
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 532 564 666
Size: 532 32 102
Fragments arranged by size:
532 102 32
PstI C_TGCA'G
Cuts at: 0 508 666
Size: 508 158
SacII CC_GC'GG
Cuts at: 0 579 666
Size: 579 87
SapI GCTCTTCn'nnn_
Cuts at: 0 29 53 666
Size: 29 24 613
Fragments arranged by size:
613 29 24
SexAI A'CCwGG_T
Cuts at: 0 395 666
Size: 395 271
SfcI C'TryA_G
Cuts at: 0 384 504 666
Size: 384 120 162
Fragments arranged by size:
384 162 120
SspI AAT'ATT
Cuts at: 0 420 666
Size: 420 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 296 666
Size: 79 217 370
Fragments arranged by size:
370 217 79
Enzymes that do cut:
AcuI AflIII AhdI AlwNI AseI AvaI BanI BanII
BbsI BcgI BmrI BplI BpmI BsaI BsaAI BsaHI
BseRI Bsp1286I BspMI BssHII BstAPI Bsu36I BtgI BtsI
DraIII EarI Eco57MI FalI Hin4I HincII HindIII MspA1I
PasI PsrI PstI SacII SapI SexAI SfcI SspI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI
BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI