Restriction map for
pFN21AB6990
(Linear) (Six-base) MAPSORT of: KIBB6990.seq Check: 9734 from: 1 to: 756
KIBB6990.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 344 756
Size: 158 186 412
Fragments arranged by size:
412 186 158
AhdI GACnn_n'nnGTC
Cuts at: 0 679 756
Size: 679 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 522 756
Size: 522 234
ApoI r'AATT_y
Cuts at: 0 445 453 756
Size: 445 8 303
Fragments arranged by size:
445 303 8
AvaI C'yCGr_G
Cuts at: 0 228 756
Size: 228 528
BanI G'GyrC_C
Cuts at: 0 24 756
Size: 24 732
BanII G_rGCy'C
Cuts at: 0 9 756
Size: 9 747
BbeI G_GCGC'C
Cuts at: 0 28 756
Size: 28 728
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 678 756
Size: 164 514 78
Fragments arranged by size:
514 164 78
BbvCI CC'TCA_GC
Cuts at: 0 704 756
Size: 704 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 756
Size: 171 585
Bpu10I CC'TnA_GC
Cuts at: 0 57 704 756
Size: 57 647 52
Fragments arranged by size:
647 57 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 756
Size: 141 615
BsaHI Gr'CG_yC
Cuts at: 0 25 756
Size: 25 731
BsiHKAI G_wGCw'C
Cuts at: 0 491 756
Size: 491 265
BsmI GAATG_Cn'
Cuts at: 0 461 478 756
Size: 461 17 278
Fragments arranged by size:
461 278 17
Bsp1286I G_dGCh'C
Cuts at: 0 9 491 756
Size: 9 482 265
Fragments arranged by size:
482 265 9
BsrFI r'CCGG_y
Cuts at: 0 21 27 756
Size: 21 6 729
Fragments arranged by size:
729 21 6
BsrGI T'GTAC_A
Cuts at: 0 741 756
Size: 741 15
BstEII G'GTnAC_C
Cuts at: 0 150 756
Size: 150 606
EarI CTCTTCn'nnn_
Cuts at: 0 248 756
Size: 248 508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 344 756
Size: 158 186 412
Fragments arranged by size:
412 186 158
HaeII r_GCGC'y
Cuts at: 0 28 756
Size: 28 728
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 82 100 114 665 697 756
Size: 68 14 18 14 551 32 59
Fragments arranged by size:
551 68 59 32 18 14 14
KasI G'GCGC_C
Cuts at: 0 24 756
Size: 24 732
MslI CAynn'nnrTG
Cuts at: 0 510 756
Size: 510 246
MspA1I CmG'CkG
Cuts at: 0 12 643 756
Size: 12 631 113
Fragments arranged by size:
631 113 12
NaeI GCC'GGC
Cuts at: 0 23 756
Size: 23 733
NarI GG'CG_CC
Cuts at: 0 25 756
Size: 25 731
NgoMIV G'CCGG_C
Cuts at: 0 21 756
Size: 21 735
NspI r_CATG'y
Cuts at: 0 509 748 756
Size: 509 239 8
Fragments arranged by size:
509 239 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 121 756
Size: 121 635
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 260 292 756
Size: 260 32 464
Fragments arranged by size:
464 260 32
PvuII CAG'CTG
Cuts at: 0 643 756
Size: 643 113
SfcI C'TryA_G
Cuts at: 0 749 756
Size: 749 7
SfoI GGC'GCC
Cuts at: 0 26 756
Size: 26 730
SgrAI Cr'CCGG_yG
Cuts at: 0 21 27 756
Size: 21 6 729
Fragments arranged by size:
729 21 6
SmlI C'TyrA_G
Cuts at: 0 156 756
Size: 156 600
SspI AAT'ATT
Cuts at: 0 526 756
Size: 526 230
TatI w'GTAC_w
Cuts at: 0 741 756
Size: 741 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 70 136 756
Size: 70 66 620
Fragments arranged by size:
620 70 66
Enzymes that do cut:
AcuI AhdI AlfI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BplI Bpu10I BpuEI BsaHI BsiHKAI BsmI
Bsp1286I BsrFI BsrGI BstEII EarI Eco57MI HaeII Hin4I
KasI MslI MspA1I NaeI NarI NgoMIV NspI PflMI
PsrI PvuII SfcI SfoI SgrAI SmlI SspI TatI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI XmnI ZraI