(Linear) (Six-base) MAPSORT of: KIBB6975.seq  Check: 7060  from: 1  to: 651



KIBB6975.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     651 
   Size:        604      47
 
AlwNI CAG_nnn'CTG

Cuts at:      0     503     651 
   Size:        503     148
 
ApoI r'AATT_y

Cuts at:      0     238     374     539     651 
   Size:        238     136     165     112

  Fragments arranged by size: 

                 238     165     136     112
 
AvaI C'yCGr_G

Cuts at:      0      30     651 
   Size:         30     621
 
BanII G_rGCy'C

Cuts at:      0     347     651 
   Size:        347     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465     651 
   Size:        465     186
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     475     651 
   Size:        475     176
 
BseYI C'CCAG_C

Cuts at:      0      75     651 
   Size:         75     576
 
BsiHKAI G_wGCw'C

Cuts at:      0     347     651 
   Size:        347     304
 
Bsp1286I G_dGCh'C

Cuts at:      0     347     651 
   Size:        347     304
 
BspHI T'CATG_A

Cuts at:      0     144     651 
   Size:        144     507
 
BsrDI GCAATG_nn'

Cuts at:      0     110     651 
   Size:        110     541
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     264     651 
   Size:        264     387
 
BtsI GCAGTG_nn'

Cuts at:      0     221     651 
   Size:        221     430
 
DraI TTT'AAA

Cuts at:      0     339     651 
   Size:        339     312
 
EaeI y'GGCC_r

Cuts at:      0     228     651 
   Size:        228     423
 
EarI CTCTTCn'nnn_

Cuts at:      0     580     628     651 
   Size:        580      48      23

  Fragments arranged by size: 

                 580      48      23
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     349     409     651 
   Size:        349      60     242

  Fragments arranged by size: 

                 349     242      60
 
EcoICRI GAG'CTC

Cuts at:      0     345     651 
   Size:        345     306
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     651 
   Size:        604      47
 
EcoRI G'AATT_C

Cuts at:      0     238     651 
   Size:        238     413
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     651 
   Size:        298      32     321

  Fragments arranged by size: 

                 321     298      32
 
MscI TGG'CCA

Cuts at:      0     230     651 
   Size:        230     421
 
MslI CAynn'nnrTG

Cuts at:      0     247     368     651 
   Size:        247     121     283

  Fragments arranged by size: 

                 283     247     121
 
MspA1I CmG'CkG

Cuts at:      0      23     651 
   Size:         23     628
 
NspI r_CATG'y

Cuts at:      0     267     651 
   Size:        267     384
 
PshAI GACnn'nnGTC

Cuts at:      0     178     651 
   Size:        178     473
 
PsiI TTA'TAA

Cuts at:      0     170     651 
   Size:        170     481
 
PstI C_TGCA'G

Cuts at:      0     492     651 
   Size:        492     159
 
PvuII CAG'CTG

Cuts at:      0      23     651 
   Size:         23     628
 
SacI G_AGCT'C

Cuts at:      0     347     651 
   Size:        347     304
 
SfcI C'TryA_G

Cuts at:      0     155     431     488     651 
   Size:        155     276      57     163

  Fragments arranged by size: 

                 276     163     155      57
 
SmaI CCC'GGG

Cuts at:      0      32     651 
   Size:         32     619
 
SmlI C'TyrA_G

Cuts at:      0     444     651 
   Size:        444     207
 
TatI w'GTAC_w

Cuts at:      0      59     260     651 
   Size:         59     201     391

  Fragments arranged by size: 

                 391     201      59
 
XmaI C'CCGG_G

Cuts at:      0      30     651 
   Size:         30     621
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI    BanII    BpuEI     BsaI    BseYI
  BsiHKAI Bsp1286I    BspHI    BsrDI   BstAPI     BtsI     DraI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoRI    Hin4I     MscI     MslI
   MspA1I     NspI    PshAI     PsiI     PstI    PvuII     SacI     SfcI
     SmaI     SmlI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI