Restriction map for
pFN21AB6958
(Linear) (Six-base) MAPSORT of: KIBB6958.seq Check: 2432 from: 1 to: 714
KIBB6958.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AatII G_ACGT'C
Cuts at: 0 536 714
Size: 536 178
AccI GT'mk_AC
Cuts at: 0 323 714
Size: 323 391
AlwNI CAG_nnn'CTG
Cuts at: 0 287 714
Size: 287 427
ApaI G_GGCC'C
Cuts at: 0 264 714
Size: 264 450
BanI G'GyrC_C
Cuts at: 0 372 714
Size: 372 342
BanII G_rGCy'C
Cuts at: 0 32 264 711 714
Size: 32 232 447 3
Fragments arranged by size:
447 232 32 3
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 192 226 642 676 714
Size: 192 34 416 34 38
Fragments arranged by size:
416 192 38 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 259 714
Size: 259 455
BglII A'GATC_T
Cuts at: 0 121 714
Size: 121 593
Bme1580I G_kGCm'C
Cuts at: 0 264 377 714
Size: 264 113 337
Fragments arranged by size:
337 264 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 714
Size: 63 651
Bpu10I CC'TnA_GC
Cuts at: 0 266 714
Size: 266 448
BsaAI yAC'GTr
Cuts at: 0 293 714
Size: 293 421
BsaHI Gr'CG_yC
Cuts at: 0 533 714
Size: 533 181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 714
Size: 178 536
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 493 714
Size: 273 220 221
Fragments arranged by size:
273 221 220
BsiEI CG_ry'CG
Cuts at: 0 254 714
Size: 254 460
BsiHKAI G_wGCw'C
Cuts at: 0 272 714
Size: 272 442
Bsp1286I G_dGCh'C
Cuts at: 0 32 264 272 377 711 714
Size: 32 232 8 105 334 3
Fragments arranged by size:
334 232 105 32 8 3
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 688 714
Size: 688 26
BstYI r'GATC_y
Cuts at: 0 121 714
Size: 121 593
BtsI GCAGTG_nn'
Cuts at: 0 110 208 660 714
Size: 110 98 452 54
Fragments arranged by size:
452 110 98 54
EaeI y'GGCC_r
Cuts at: 0 251 460 714
Size: 251 209 254
Fragments arranged by size:
254 251 209
EagI C'GGCC_G
Cuts at: 0 251 714
Size: 251 463
EarI CTCTTCn'nnn_
Cuts at: 0 134 569 714
Size: 134 435 145
Fragments arranged by size:
435 145 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 33 714
Size: 33 681
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 714
Size: 697 17
EcoO109I rG'GnC_Cy
Cuts at: 0 105 260 261 714
Size: 105 155 1 453
Fragments arranged by size:
453 155 105 1
HaeII r_GCGC'y
Cuts at: 0 288 714
Size: 288 426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 342 374 650 682 714
Size: 342 32 276 32 32
Fragments arranged by size:
342 276 32 32 32
HindIII A'AGCT_T
Cuts at: 0 402 714
Size: 402 312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 714
Size: 413 301
MspA1I CmG'CkG
Cuts at: 0 689 714
Size: 689 25
PasI CC'CwG_GG
Cuts at: 0 235 714
Size: 235 479
PflMI CCAn_nnn'nTGG
Cuts at: 0 226 528 714
Size: 226 302 186
Fragments arranged by size:
302 226 186
PmlI CAC'GTG
Cuts at: 0 293 714
Size: 293 421
PspOMI G'GGCC_C
Cuts at: 0 260 714
Size: 260 454
PstI C_TGCA'G
Cuts at: 0 117 215 449 714
Size: 117 98 234 265
Fragments arranged by size:
265 234 117 98
SfcI C'TryA_G
Cuts at: 0 113 211 393 445 714
Size: 113 98 182 52 269
Fragments arranged by size:
269 182 113 98 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 259 714
Size: 259 455
StuI AGG'CCT
Cuts at: 0 502 714
Size: 502 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 714
Size: 535 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 301 333 714
Size: 262 32 7 32 381
Fragments arranged by size:
381 262 32 32 7
ZraI GAC'GTC
Cuts at: 0 534 714
Size: 534 180
Enzymes that do cut:
AatII AccI AlwNI ApaI BanI BanII BcgI BglI
BglII Bme1580I BmrI Bpu10I BsaAI BsaHI BseRI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BstYI BtsI EaeI EagI
EarI EciI EcoNI EcoO109I HaeII Hin4I HindIII MmeI
MspA1I PasI PflMI PmlI PspOMI PstI SfcI SfiI
StuI TaqII TstI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI BpmI
BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI Eco57MI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI