(Linear) (Six-base) MAPSORT of: KIBB6945.seq  Check: 2705  from: 1  to: 633



KIBB6945.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     207     305     633 
   Size:        207      98     328

  Fragments arranged by size: 

                 328     207      98
 
AccI GT'mk_AC

Cuts at:      0     425     476     633 
   Size:        425      51     157

  Fragments arranged by size: 

                 425     157      51
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     633 
   Size:        167     466
 
AflIII A'CryG_T

Cuts at:      0     420     633 
   Size:        420     213
 
AlwNI CAG_nnn'CTG

Cuts at:      0     297     633 
   Size:        297     336
 
AvrII C'CTAG_G

Cuts at:      0     450     633 
   Size:        450     183
 
BglI GCCn_nnn'nGGC

Cuts at:      0     443     633 
   Size:        443     190
 
BmgBI CAC'GTC

Cuts at:      0     423     633 
   Size:        423     210
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     109     633 
   Size:         85      24     524

  Fragments arranged by size: 

                 524      85      24
 
BseYI C'CCAG_C

Cuts at:      0     527     633 
   Size:        527     106
 
BsmI GAATG_Cn'

Cuts at:      0     321     633 
   Size:        321     312
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      66     207     305     633 
   Size:         66     141      98     328

  Fragments arranged by size: 

                 328     141      98      66
 
BsrDI GCAATG_nn'

Cuts at:      0     497     558     633 
   Size:        497      61      75

  Fragments arranged by size: 

                 497      75      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297     316     633 
   Size:        297      19     317

  Fragments arranged by size: 

                 317     297      19
 
BstEII G'GTnAC_C

Cuts at:      0     180     459     633 
   Size:        180     279     174

  Fragments arranged by size: 

                 279     180     174
 
BtgI C'CryG_G

Cuts at:      0     209     305     633 
   Size:        209      96     328

  Fragments arranged by size: 

                 328     209      96
 
BtsI GCAGTG_nn'

Cuts at:      0     492     633 
   Size:        492     141
 
EarI CTCTTCn'nnn_

Cuts at:      0     395     633 
   Size:        395     238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     633 
   Size:        167     466
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     454     633 
   Size:        454     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0      22     633 
   Size:         22     611
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     215     247     633 
   Size:        215      32     386

  Fragments arranged by size: 

                 386     215      32
 
HincII GTy'rAC

Cuts at:      0     477     633 
   Size:        477     156
 
MfeI C'AATT_G

Cuts at:      0     369     633 
   Size:        369     264
 
NcoI C'CATG_G

Cuts at:      0     209     633 
   Size:        209     424
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     609     633 
   Size:        609      24
 
PpuMI rG'GwC_Cy

Cuts at:      0      22     633 
   Size:         22     611
 
PstI C_TGCA'G

Cuts at:      0     302     633 
   Size:        302     331
 
SalI G'TCGA_C

Cuts at:      0     475     633 
   Size:        475     158
 
SapI GCTCTTCn'nnn_

Cuts at:      0     395     633 
   Size:        395     238
 
ScaI AGT'ACT

Cuts at:      0     328     633 
   Size:        328     305
 
SfcI C'TryA_G

Cuts at:      0     298     633 
   Size:        298     335
 
StyI C'CwwG_G

Cuts at:      0     209     450     633 
   Size:        209     241     183

  Fragments arranged by size: 

                 241     209     183
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     519     574     633 
   Size:        519      55      59

  Fragments arranged by size: 

                 519      59      55
 
TatI w'GTAC_w

Cuts at:      0     326     633 
   Size:        326     307
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216     633 
   Size:        216     417
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI    AvrII     BglI    BmgBI
    BseRI    BseYI     BsmI    BspMI    BsrDI   BstAPI   BstEII     BtgI
     BtsI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MfeI
     NcoI    PflMI    PpuMI     PstI     SalI     SapI     ScaI     SfcI
     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI