(Linear) (Six-base) MAPSORT of: KIBB6931.seq  Check: 7765  from: 1  to: 621



KIBB6931.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:36  ..

AatII G_ACGT'C

Cuts at:      0     341     621 
   Size:        341     280
 
AccI GT'mk_AC

Cuts at:      0     260     621 
   Size:        260     361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     347     621 
   Size:        347     274
 
AvaI C'yCGr_G

Cuts at:      0      48     355     621 
   Size:         48     307     266

  Fragments arranged by size: 

                 307     266      48
 
BanII G_rGCy'C

Cuts at:      0     155     621 
   Size:        155     466
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      13      67     621 
   Size:         13      54     554

  Fragments arranged by size: 

                 554      54      13
 
BfrBI ATG'CAT

Cuts at:      0     481     621 
   Size:        481     140
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     345     621 
   Size:        345     276
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     439     621 
   Size:        439     182
 
BsaHI Gr'CG_yC

Cuts at:      0      92     338     621 
   Size:         92     246     283

  Fragments arranged by size: 

                 283     246      92
 
BsaWI w'CCGG_w

Cuts at:      0     296     576     621 
   Size:        296     280      45

  Fragments arranged by size: 

                 296     280      45
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     334     606     621 
   Size:        334     272      15

  Fragments arranged by size: 

                 334     272      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     155     326     621 
   Size:        155     171     295

  Fragments arranged by size: 

                 295     171     155
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     326     621 
   Size:        155     171     295

  Fragments arranged by size: 

                 295     171     155
 
BspEI T'CCGG_A

Cuts at:      0     296     621 
   Size:        296     325
 
BsrDI GCAATG_nn'

Cuts at:      0     248     621 
   Size:        248     373
 
BsrFI r'CCGG_y

Cuts at:      0     193     621 
   Size:        193     428
 
DraI TTT'AAA

Cuts at:      0     135     621 
   Size:        135     486
 
EarI CTCTTCn'nnn_

Cuts at:      0     583     621 
   Size:        583      38
 
EcoICRI GAG'CTC

Cuts at:      0     153     621 
   Size:        153     468
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     231     621 
   Size:        231     390
 
EcoRV GAT'ATC

Cuts at:      0     504     621 
   Size:        504     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     554     586     621 
   Size:        554      32      35

  Fragments arranged by size: 

                 554      35      32
 
MslI CAynn'nnrTG

Cuts at:      0     469     621 
   Size:        469     152
 
NsiI A_TGCA'T

Cuts at:      0     483     621 
   Size:        483     138
 
PasI CC'CwG_GG

Cuts at:      0     546     621 
   Size:        546      75
 
PshAI GACnn'nnGTC

Cuts at:      0      67     621 
   Size:         67     554
 
PstI C_TGCA'G

Cuts at:      0     179     336     621 
   Size:        179     157     285

  Fragments arranged by size: 

                 285     179     157
 
SacI G_AGCT'C

Cuts at:      0     155     621 
   Size:        155     466
 
SfcI C'TryA_G

Cuts at:      0     175     231     332     621 
   Size:        175      56     101     289

  Fragments arranged by size: 

                 289     175     101      56
 
StyI C'CwwG_G

Cuts at:      0     395     621 
   Size:        395     226
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      51      83     351     383     621 
   Size:         51      32     268      32     238

  Fragments arranged by size: 

                 268     238      51      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     468     621 
   Size:        468     153
 
XmnI GAAnn'nnTTC

Cuts at:      0     206     283     621 
   Size:        206      77     338

  Fragments arranged by size: 

                 338     206      77
 
ZraI GAC'GTC

Cuts at:      0     339     621 
   Size:        339     282
 

 Enzymes that do cut:

    AatII     AccI     AlfI     AvaI    BanII     BbsI    BfrBI     BplI
     BsaI    BsaHI    BsaWI    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI
    BsrFI     DraI     EarI  EcoICRI    EcoNI    EcoRV    Hin4I     MslI
     NsiI     PasI    PshAI     PstI     SacI     SfcI     StyI     TstI
     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  Eco57MI EcoO109I    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI
     XmaI