Restriction map for
pFN21AB6868
(Linear) (Six-base) MAPSORT of: KIBB6868.seq Check: 2275 from: 1 to: 588
KIBB6868.orf
With 182 enzymes: SgfI *
March 22, 2012 15:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 493 588
Size: 493 95
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 527 559 588
Size: 527 32 29
Fragments arranged by size:
527 32 29
AlwNI CAG_nnn'CTG
Cuts at: 0 404 588
Size: 404 184
ApaI G_GGCC'C
Cuts at: 0 186 398 588
Size: 186 212 190
Fragments arranged by size:
212 190 186
BamHI G'GATC_C
Cuts at: 0 238 588
Size: 238 350
BanI G'GyrC_C
Cuts at: 0 149 195 338 415 588
Size: 149 46 143 77 173
Fragments arranged by size:
173 149 143 77 46
BanII G_rGCy'C
Cuts at: 0 186 398 588
Size: 186 212 190
Fragments arranged by size:
212 190 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 588
Size: 254 334
BclI T'GATC_A
Cuts at: 0 297 588
Size: 297 291
BfrBI ATG'CAT
Cuts at: 0 472 588
Size: 472 116
Bme1580I G_kGCm'C
Cuts at: 0 154 186 200 398 420 588
Size: 154 32 14 198 22 168
Fragments arranged by size:
198 168 154 32 22 14
BmgBI CAC'GTC
Cuts at: 0 329 588
Size: 329 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 588
Size: 467 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 298 588
Size: 298 290
BsaWI w'CCGG_w
Cuts at: 0 235 588
Size: 235 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 527 588
Size: 476 51 61
Fragments arranged by size:
476 61 51
BsiHKAI G_wGCw'C
Cuts at: 0 455 588
Size: 455 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 335 588
Size: 335 253
Bsp1286I G_dGCh'C
Cuts at: 0 154 186 200 398 420 455 588
Size: 154 32 14 198 22 35 133
Fragments arranged by size:
198 154 133 35 32 22 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 493 588
Size: 493 95
BsrDI GCAATG_nn'
Cuts at: 0 472 588
Size: 472 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 588
Size: 162 426
BstYI r'GATC_y
Cuts at: 0 238 588
Size: 238 350
BtgI C'CryG_G
Cuts at: 0 117 588
Size: 117 471
BtsI GCAGTG_nn'
Cuts at: 0 457 514 588
Size: 457 57 74
Fragments arranged by size:
457 74 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 455 490 588
Size: 455 35 98
Fragments arranged by size:
455 98 35
EaeI y'GGCC_r
Cuts at: 0 32 50 588
Size: 32 18 538
Fragments arranged by size:
538 32 18
EarI CTCTTCn'nnn_
Cuts at: 0 251 588
Size: 251 337
EcoO109I rG'GnC_Cy
Cuts at: 0 182 269 401 588
Size: 182 87 132 187
Fragments arranged by size:
187 182 132 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 588
Size: 367 32 189
Fragments arranged by size:
367 189 32
MscI TGG'CCA
Cuts at: 0 34 52 588
Size: 34 18 536
Fragments arranged by size:
536 34 18
MslI CAynn'nnrTG
Cuts at: 0 353 588
Size: 353 235
MspA1I CmG'CkG
Cuts at: 0 436 588
Size: 436 152
NcoI C'CATG_G
Cuts at: 0 117 588
Size: 117 471
NsiI A_TGCA'T
Cuts at: 0 474 588
Size: 474 114
NspI r_CATG'y
Cuts at: 0 472 588
Size: 472 116
PasI CC'CwG_GG
Cuts at: 0 398 588
Size: 398 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 223 588
Size: 223 365
PpuMI rG'GwC_Cy
Cuts at: 0 269 588
Size: 269 319
PspOMI G'GGCC_C
Cuts at: 0 182 394 588
Size: 182 212 194
Fragments arranged by size:
212 194 182
PstI C_TGCA'G
Cuts at: 0 79 390 588
Size: 79 311 198
Fragments arranged by size:
311 198 79
SfcI C'TryA_G
Cuts at: 0 75 386 405 588
Size: 75 311 19 183
Fragments arranged by size:
311 183 75 19
SmlI C'TyrA_G
Cuts at: 0 482 588
Size: 482 106
StyI C'CwwG_G
Cuts at: 0 117 334 588
Size: 117 217 254
Fragments arranged by size:
254 217 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 36 588
Size: 36 552
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 588
Size: 223 32 333
Fragments arranged by size:
333 223 32
Enzymes that do cut:
AarI AloI AlwNI ApaI BamHI BanI BanII BbsI
BclI BfrBI Bme1580I BmgBI BpuEI BsaI BsaWI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI
BtsI CspCI EaeI EarI EcoO109I Hin4I MscI MslI
MspA1I NcoI NsiI NspI PasI PflMI PpuMI PspOMI
PstI SfcI SmlI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BglI BglII BlpI BmrI BmtI BplI BpmI Bpu10I
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI