Restriction map for
pFN21AB6670
(Linear) (Six-base) MAPSORT of: KIBB6670.seq Check: 456 from: 1 to: 1362
KIBB6670.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
Acc65I G'GTAC_C
Cuts at: 0 1097 1362
Size: 1097 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 705 910 925 1221 1362
Size: 439 266 205 15 296 141
Fragments arranged by size:
439 296 266 205 141 15
AfeI AGC'GCT
Cuts at: 0 970 1362
Size: 970 392
AflIII A'CryG_T
Cuts at: 0 596 898 1362
Size: 596 302 464
Fragments arranged by size:
596 464 302
AhdI GACnn_n'nnGTC
Cuts at: 0 899 1362
Size: 899 463
AlwNI CAG_nnn'CTG
Cuts at: 0 369 807 1131 1152 1362
Size: 369 438 324 21 210
Fragments arranged by size:
438 369 324 210 21
AvaI C'yCGr_G
Cuts at: 0 874 1362
Size: 874 488
BanI G'GyrC_C
Cuts at: 0 215 535 884 1097 1362
Size: 215 320 349 213 265
Fragments arranged by size:
349 320 265 215 213
BanII G_rGCy'C
Cuts at: 0 302 1362
Size: 302 1060
BbeI G_GCGC'C
Cuts at: 0 888 1362
Size: 888 474
BbsI GAAGACnn'nnnn_
Cuts at: 0 898 1362
Size: 898 464
BglI GCCn_nnn'nGGC
Cuts at: 0 879 1362
Size: 879 483
Bme1580I G_kGCm'C
Cuts at: 0 103 458 1362
Size: 103 355 904
Fragments arranged by size:
904 355 103
BmgBI CAC'GTC
Cuts at: 0 24 264 1056 1362
Size: 24 240 792 306
Fragments arranged by size:
792 306 240 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 345 1362
Size: 345 1017
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 303 990 1362
Size: 303 687 372
Fragments arranged by size:
687 372 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 607 915 1006 1267 1342 1362
Size: 298 309 308 91 261 75 20
Fragments arranged by size:
309 308 298 261 91 75 20
Bpu10I CC'TnA_GC
Cuts at: 0 631 1362
Size: 631 731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1141 1156 1210 1362
Size: 343 798 15 54 152
Fragments arranged by size:
798 343 152 54 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 291 652 1362
Size: 291 361 710
Fragments arranged by size:
710 361 291
BsaHI Gr'CG_yC
Cuts at: 0 885 1362
Size: 885 477
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 594 624 1081 1111 1362
Size: 310 30 254 30 457 30 251
Fragments arranged by size:
457 310 254 251 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 190 727 1003 1021 1096 1120 1204
Size: 64 126 537 276 18 75 24 84
Cuts at: 1204 1362
Size: 158
Fragments arranged by size:
537 276 158 126 84 75 64 24
18
BseYI C'CCAG_C
Cuts at: 0 54 579 1209 1362
Size: 54 525 630 153
Fragments arranged by size:
630 525 153 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 373 1321 1362
Size: 220 153 948 41
Fragments arranged by size:
948 220 153 41
BsiEI CG_ry'CG
Cuts at: 0 77 867 1001 1362
Size: 77 790 134 361
Fragments arranged by size:
790 361 134 77
BsiHKAI G_wGCw'C
Cuts at: 0 302 1362
Size: 302 1060
BsmBI CGTCTCn'nnnn_
Cuts at: 0 664 1362
Size: 664 698
Bsp1286I G_dGCh'C
Cuts at: 0 103 302 458 1362
Size: 103 199 156 904
Fragments arranged by size:
904 199 156 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 1139 1362
Size: 395 744 223
Fragments arranged by size:
744 395 223
BsrBI CCG'CTC
Cuts at: 0 186 635 879 1362
Size: 186 449 244 483
Fragments arranged by size:
483 449 244 186
BsrDI GCAATG_nn'
Cuts at: 0 794 1362
Size: 794 568
BsrFI r'CCGG_y
Cuts at: 0 89 155 758 1034 1362
Size: 89 66 603 276 328
Fragments arranged by size:
603 328 276 89 66
BssHII G'CGCG_C
Cuts at: 0 1202 1314 1362
Size: 1202 112 48
Fragments arranged by size:
1202 112 48
BssSI C'ACGA_G
Cuts at: 0 1048 1309 1362
Size: 1048 261 53
Fragments arranged by size:
1048 261 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1152 1362
Size: 1152 210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 984 1362
Size: 738 246 378
Fragments arranged by size:
738 378 246
BstYI r'GATC_y
Cuts at: 0 209 365 774 1362
Size: 209 156 409 588
Fragments arranged by size:
588 409 209 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 474 1362
Size: 387 87 888
Fragments arranged by size:
888 387 87
EaeI y'GGCC_r
Cuts at: 0 63 740 864 998 1067 1268 1362
Size: 63 677 124 134 69 201 94
Fragments arranged by size:
677 201 134 124 94 69 63
EagI C'GGCC_G
Cuts at: 0 864 998 1362
Size: 864 134 364
Fragments arranged by size:
864 364 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 607 855 1362
Size: 331 276 248 507
Fragments arranged by size:
507 331 276 248
EcoICRI GAG'CTC
Cuts at: 0 300 1362
Size: 300 1062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 439 607 705 910 915 925 1006
Size: 298 141 168 98 205 5 10 81
Cuts at: 1006 1221 1267 1342 1362
Size: 215 46 75 20
Fragments arranged by size:
298 215 205 168 141 98 81 75
46 20 10 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1123 1324 1362
Size: 1123 201 38
Fragments arranged by size:
1123 201 38
EcoO109I rG'GnC_Cy
Cuts at: 0 1011 1128 1305 1362
Size: 1011 117 177 57
Fragments arranged by size:
1011 177 117 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 546 1251 1362
Size: 546 705 111
Fragments arranged by size:
705 546 111
HaeII r_GCGC'y
Cuts at: 0 141 888 972 1066 1171 1233 1267 1362
Size: 141 747 84 94 105 62 34 95
Fragments arranged by size:
747 141 105 95 94 84 62 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 885 917 1362
Size: 310 32 543 32 445
Fragments arranged by size:
543 445 310 32 32
KasI G'GCGC_C
Cuts at: 0 884 1362
Size: 884 478
KpnI G_GTAC'C
Cuts at: 0 1101 1362
Size: 1101 261
MluI A'CGCG_T
Cuts at: 0 898 1362
Size: 898 464
MscI TGG'CCA
Cuts at: 0 742 1270 1362
Size: 742 528 92
Fragments arranged by size:
742 528 92
MspA1I CmG'CkG
Cuts at: 0 62 579 863 1220 1362
Size: 62 517 284 357 142
Fragments arranged by size:
517 357 284 142 62
NaeI GCC'GGC
Cuts at: 0 91 157 760 1036 1362
Size: 91 66 603 276 326
Fragments arranged by size:
603 326 276 91 66
NarI GG'CG_CC
Cuts at: 0 885 1362
Size: 885 477
NgoMIV G'CCGG_C
Cuts at: 0 89 155 758 1034 1362
Size: 89 66 603 276 328
Fragments arranged by size:
603 328 276 89 66
NotI GC'GGCC_GC
Cuts at: 0 864 998 1362
Size: 864 134 364
Fragments arranged by size:
864 364 134
NspI r_CATG'y
Cuts at: 0 600 1362
Size: 600 762
PciI A'CATG_T
Cuts at: 0 596 1362
Size: 596 766
PflMI CCAn_nnn'nTGG
Cuts at: 0 807 1362
Size: 807 555
PfoI T'CCnGG_A
Cuts at: 0 275 368 817 1362
Size: 275 93 449 545
Fragments arranged by size:
545 449 275 93
PpuMI rG'GwC_Cy
Cuts at: 0 1128 1305 1362
Size: 1128 177 57
Fragments arranged by size:
1128 177 57
PstI C_TGCA'G
Cuts at: 0 1028 1340 1362
Size: 1028 312 22
Fragments arranged by size:
1028 312 22
PvuI CG_AT'CG
Cuts at: 0 77 1362
Size: 77 1285
SacI G_AGCT'C
Cuts at: 0 302 1362
Size: 302 1060
ScaI AGT'ACT
Cuts at: 0 718 1362
Size: 718 644
SfcI C'TryA_G
Cuts at: 0 1024 1336 1362
Size: 1024 312 26
Fragments arranged by size:
1024 312 26
SfoI GGC'GCC
Cuts at: 0 886 1362
Size: 886 476
SmlI C'TyrA_G
Cuts at: 0 358 1156 1171 1225 1362
Size: 358 798 15 54 137
Fragments arranged by size:
798 358 137 54 15
StuI AGG'CCT
Cuts at: 0 1093 1362
Size: 1093 269
StyI C'CwwG_G
Cuts at: 0 743 977 1196 1362
Size: 743 234 219 166
Fragments arranged by size:
743 234 219 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 1177 1362
Size: 364 813 185
Fragments arranged by size:
813 364 185
TatI w'GTAC_w
Cuts at: 0 716 911 1362
Size: 716 195 451
Fragments arranged by size:
716 451 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 594 626 834 866 1362
Size: 594 32 208 32 496
Fragments arranged by size:
594 496 208 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 265 1362
Size: 265 1097
XmnI GAAnn'nnTTC
Cuts at: 0 517 529 1362
Size: 517 12 833
Fragments arranged by size:
833 517 12
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI AlwNI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgZI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI KpnI MluI MscI MspA1I NaeI NarI
NgoMIV NotI NspI PciI PflMI PfoI PpuMI PstI
PvuI SacI ScaI SfcI SfoI SmlI StuI StyI
TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BsaAI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrGI BstBI BstEII BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII DrdI EarI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI MfeI MmeI
MslI NcoI NdeI NheI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XcmI XhoI XmaI ZraI