Restriction map for
pFN21AB6661
(Linear) (Six-base) MAPSORT of: KIBB6661.seq Check: 8547 from: 1 to: 1272
KIBB6661.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AatII G_ACGT'C
Cuts at: 0 247 1272
Size: 247 1025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1272
Size: 1196 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 538 1272
Size: 538 734
AlwNI CAG_nnn'CTG
Cuts at: 0 204 446 712 1272
Size: 204 242 266 560
Fragments arranged by size:
560 266 242 204
ApaI G_GGCC'C
Cuts at: 0 229 1272
Size: 229 1043
ApoI r'AATT_y
Cuts at: 0 348 386 1202 1272
Size: 348 38 816 70
Fragments arranged by size:
816 348 70 38
AvaI C'yCGr_G
Cuts at: 0 34 229 1272
Size: 34 195 1043
Fragments arranged by size:
1043 195 34
AvrII C'CTAG_G
Cuts at: 0 75 1272
Size: 75 1197
BamHI G'GATC_C
Cuts at: 0 425 1272
Size: 425 847
BanII G_rGCy'C
Cuts at: 0 176 229 1080 1272
Size: 176 53 851 192
Fragments arranged by size:
851 192 176 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 744 1272
Size: 744 528
BbvCI CC'TCA_GC
Cuts at: 0 1070 1272
Size: 1070 202
BclI T'GATC_A
Cuts at: 0 782 1272
Size: 782 490
BlpI GC'TnA_GC
Cuts at: 0 124 1272
Size: 124 1148
Bme1580I G_kGCm'C
Cuts at: 0 229 1272
Size: 229 1043
Bpu10I CC'TnA_GC
Cuts at: 0 1070 1162 1272
Size: 1070 92 110
Fragments arranged by size:
1070 110 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1115 1272
Size: 889 226 157
Fragments arranged by size:
889 226 157
BsaI GGTCTCn'nnnn_
Cuts at: 0 1259 1272
Size: 1259 13
BsaBI GATnn'nnATC
Cuts at: 0 424 482 787 1272
Size: 424 58 305 485
Fragments arranged by size:
485 424 305 58
BsaHI Gr'CG_yC
Cuts at: 0 244 1272
Size: 244 1028
BsaWI w'CCGG_w
Cuts at: 0 320 494 1272
Size: 320 174 778
Fragments arranged by size:
778 320 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1027 1057 1272
Size: 1027 30 215
Fragments arranged by size:
1027 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 780 1272
Size: 780 492
BseYI C'CCAG_C
Cuts at: 0 186 1272
Size: 186 1086
BsiEI CG_ry'CG
Cuts at: 0 213 1272
Size: 213 1059
BsiHKAI G_wGCw'C
Cuts at: 0 517 1272
Size: 517 755
BsmBI CGTCTCn'nnnn_
Cuts at: 0 369 1272
Size: 369 903
Bsp1286I G_dGCh'C
Cuts at: 0 176 229 517 1080 1272
Size: 176 53 288 563 192
Fragments arranged by size:
563 288 192 176 53
BspEI T'CCGG_A
Cuts at: 0 494 1272
Size: 494 778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 665 1272
Size: 665 607
BsrBI CCG'CTC
Cuts at: 0 615 1272
Size: 615 657
BsrFI r'CCGG_y
Cuts at: 0 14 215 252 1272
Size: 14 201 37 1020
Fragments arranged by size:
1020 201 37 14
BsrGI T'GTAC_A
Cuts at: 0 437 1272
Size: 437 835
BssHII G'CGCG_C
Cuts at: 0 273 1272
Size: 273 999
BssSI C'ACGA_G
Cuts at: 0 118 676 1272
Size: 118 558 596
Fragments arranged by size:
596 558 118
BstAPI GCAn_nnn'nTGC
Cuts at: 0 120 204 478 1272
Size: 120 84 274 794
Fragments arranged by size:
794 274 120 84
BstEII G'GTnAC_C
Cuts at: 0 1240 1272
Size: 1240 32
BstYI r'GATC_y
Cuts at: 0 425 788 1037 1272
Size: 425 363 249 235
Fragments arranged by size:
425 363 249 235
BtgI C'CryG_G
Cuts at: 0 59 136 221 1260 1272
Size: 59 77 85 1039 12
Fragments arranged by size:
1039 85 77 59 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 433 1272
Size: 433 839
BtsI GCAGTG_nn'
Cuts at: 0 479 722 1208 1272
Size: 479 243 486 64
Fragments arranged by size:
486 479 243 64
DraIII CAC_nnn'GTG
Cuts at: 0 332 1272
Size: 332 940
EaeI y'GGCC_r
Cuts at: 0 210 551 1272
Size: 210 341 721
Fragments arranged by size:
721 341 210
EagI C'GGCC_G
Cuts at: 0 210 1272
Size: 210 1062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1272
Size: 1196 76
EcoO109I rG'GnC_Cy
Cuts at: 0 22 80 226 233 546 618 1272
Size: 22 58 146 7 313 72 654
Fragments arranged by size:
654 313 146 72 58 22 7
EcoRV GAT'ATC
Cuts at: 0 885 1272
Size: 885 387
HaeII r_GCGC'y
Cuts at: 0 187 1272
Size: 187 1085
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 767 799 1272
Size: 349 32 386 32 473
Fragments arranged by size:
473 386 349 32 32
HincII GTy'rAC
Cuts at: 0 357 1272
Size: 357 915
MfeI C'AATT_G
Cuts at: 0 1196 1272
Size: 1196 76
MscI TGG'CCA
Cuts at: 0 553 1272
Size: 553 719
MspA1I CmG'CkG
Cuts at: 0 138 167 201 223 283 432 1272
Size: 138 29 34 22 60 149 840
Fragments arranged by size:
840 149 138 60 34 29 22
NaeI GCC'GGC
Cuts at: 0 16 217 254 1272
Size: 16 201 37 1018
Fragments arranged by size:
1018 201 37 16
NcoI C'CATG_G
Cuts at: 0 59 1260 1272
Size: 59 1201 12
Fragments arranged by size:
1201 59 12
NgoMIV G'CCGG_C
Cuts at: 0 14 215 252 1272
Size: 14 201 37 1020
Fragments arranged by size:
1020 201 37 14
NspI r_CATG'y
Cuts at: 0 1170 1272
Size: 1170 102
PasI CC'CwG_GG
Cuts at: 0 963 1272
Size: 963 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 332 1261 1272
Size: 332 929 11
Fragments arranged by size:
929 332 11
PfoI T'CCnGG_A
Cuts at: 0 1117 1272
Size: 1117 155
PpuMI rG'GwC_Cy
Cuts at: 0 22 80 233 1272
Size: 22 58 153 1039
Fragments arranged by size:
1039 153 58 22
PspOMI G'GGCC_C
Cuts at: 0 225 1272
Size: 225 1047
PstI C_TGCA'G
Cuts at: 0 200 717 921 1272
Size: 200 517 204 351
Fragments arranged by size:
517 351 204 200
PvuII CAG'CTG
Cuts at: 0 201 432 1272
Size: 201 231 840
Fragments arranged by size:
840 231 201
RsrII CG'GwC_CG
Cuts at: 0 261 1272
Size: 261 1011
SacII CC_GC'GG
Cuts at: 0 139 224 1272
Size: 139 85 1048
Fragments arranged by size:
1048 139 85
SanDI GG'GwC_CC
Cuts at: 0 233 1272
Size: 233 1039
SfcI C'TryA_G
Cuts at: 0 196 713 917 1272
Size: 196 517 204 355
Fragments arranged by size:
517 355 204 196
SmaI CCC'GGG
Cuts at: 0 231 1272
Size: 231 1041
SmlI C'TyrA_G
Cuts at: 0 868 1130 1272
Size: 868 262 142
Fragments arranged by size:
868 262 142
SphI G_CATG'C
Cuts at: 0 1170 1272
Size: 1170 102
StyI C'CwwG_G
Cuts at: 0 59 75 1260 1272
Size: 59 16 1185 12
Fragments arranged by size:
1185 59 16 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 1097 1126 1272
Size: 249 848 29 146
Fragments arranged by size:
848 249 146 29
TatI w'GTAC_w
Cuts at: 0 437 1272
Size: 437 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1272
Size: 1027 32 213
Fragments arranged by size:
1027 213 32
Tth111I GACn'n_nGTC
Cuts at: 0 361 1272
Size: 361 911
XmaI C'CCGG_G
Cuts at: 0 229 1272
Size: 229 1043
ZraI GAC'GTC
Cuts at: 0 245 1272
Size: 245 1027
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI ApoI AvaI AvrII
BamHI BanII BbsI BbvCI BclI BlpI Bme1580I Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstYI BtgI BtgZI
BtsI DraIII EaeI EagI Eco57MI EcoO109I EcoRV HaeII
Hin4I HincII MfeI MscI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
RsrII SacII SanDI SfcI SmaI SmlI SphI StyI
TaqII TatI TstI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI
BanI BbeI BcgI BciVI BfrBI BglI BglII BmgBI
BmrI BmtI BplI BpmI BsaAI BsgI BsiWI BsmI
BspHI BsrDI BstBI BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
MmeI MslI NarI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI SacI SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI XbaI XcmI XhoI XmnI