Restriction map for
pFN21AB6644
(Linear) (Six-base) MAPSORT of: KIBB6644.seq Check: 9772 from: 1 to: 1329
KIBB6644.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1329
Size: 1288 41
AgeI A'CCGG_T
Cuts at: 0 125 1329
Size: 125 1204
AlwNI CAG_nnn'CTG
Cuts at: 0 372 1329
Size: 372 957
ApoI r'AATT_y
Cuts at: 0 757 1329
Size: 757 572
AvaI C'yCGr_G
Cuts at: 0 131 164 926 989 1329
Size: 131 33 762 63 340
Fragments arranged by size:
762 340 131 63 33
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 938 971 1329
Size: 938 33 358
Fragments arranged by size:
938 358 33
BamHI G'GATC_C
Cuts at: 0 148 1329
Size: 148 1181
BanI G'GyrC_C
Cuts at: 0 73 160 855 976 1329
Size: 73 87 695 121 353
Fragments arranged by size:
695 353 121 87 73
BanII G_rGCy'C
Cuts at: 0 604 1329
Size: 604 725
BbeI G_GCGC'C
Cuts at: 0 164 1329
Size: 164 1165
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 678 1329
Size: 32 646 651
Fragments arranged by size:
651 646 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 237 271 1329
Size: 237 34 1058
Fragments arranged by size:
1058 237 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 509 1055 1329
Size: 509 546 274
Fragments arranged by size:
546 509 274
BglI GCCn_nnn'nGGC
Cuts at: 0 53 105 1329
Size: 53 52 1224
Fragments arranged by size:
1224 53 52
BglII A'GATC_T
Cuts at: 0 581 1329
Size: 581 748
Bme1580I G_kGCm'C
Cuts at: 0 78 1329
Size: 78 1251
BmgBI CAC'GTC
Cuts at: 0 850 1329
Size: 850 479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 351 1040 1329
Size: 351 689 289
Fragments arranged by size:
689 351 289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1329
Size: 1010 319
BsaI GGTCTCn'nnnn_
Cuts at: 0 641 1329
Size: 641 688
BsaAI yAC'GTr
Cuts at: 0 943 1329
Size: 943 386
BsaHI Gr'CG_yC
Cuts at: 0 97 161 1329
Size: 97 64 1168
Fragments arranged by size:
1168 97 64
BsaWI w'CCGG_w
Cuts at: 0 70 125 768 1329
Size: 70 55 643 561
Fragments arranged by size:
643 561 70 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 875 1329
Size: 439 436 454
Fragments arranged by size:
454 439 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1067 1329
Size: 863 204 262
Fragments arranged by size:
863 262 204
BsiHKAI G_wGCw'C
Cuts at: 0 1026 1041 1313 1329
Size: 1026 15 272 16
Fragments arranged by size:
1026 272 16 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 107 1329
Size: 107 1222
Bsp1286I G_dGCh'C
Cuts at: 0 78 604 1026 1041 1313 1329
Size: 78 526 422 15 272 16
Fragments arranged by size:
526 422 272 78 16 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 1329
Size: 317 1012
BsrFI r'CCGG_y
Cuts at: 0 35 125 1329
Size: 35 90 1204
Fragments arranged by size:
1204 90 35
BssHII G'CGCG_C
Cuts at: 0 25 1329
Size: 25 1304
BssSI C'ACGA_G
Cuts at: 0 842 1329
Size: 842 487
BstYI r'GATC_y
Cuts at: 0 148 581 675 1329
Size: 148 433 94 654
Fragments arranged by size:
654 433 148 94
BtgI C'CryG_G
Cuts at: 0 59 153 212 740 909 1329
Size: 59 94 59 528 169 420
Fragments arranged by size:
528 420 169 94 59 59
DrdI GACnn_nn'nnGTC
Cuts at: 0 1260 1329
Size: 1260 69
EaeI y'GGCC_r
Cuts at: 0 209 269 323 1233 1329
Size: 209 60 54 910 96
Fragments arranged by size:
910 209 96 60 54
EarI CTCTTCn'nnn_
Cuts at: 0 560 623 1329
Size: 560 63 706
Fragments arranged by size:
706 560 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 155 1329
Size: 155 1174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1288 1329
Size: 1010 278 41
Fragments arranged by size:
1010 278 41
EcoO109I rG'GnC_Cy
Cuts at: 0 217 1272 1329
Size: 217 1055 57
Fragments arranged by size:
1055 217 57
EcoRV GAT'ATC
Cuts at: 0 786 1329
Size: 786 543
FspI TGC'GCA
Cuts at: 0 637 1329
Size: 637 692
HaeII r_GCGC'y
Cuts at: 0 9 164 247 1329
Size: 9 155 83 1082
Fragments arranged by size:
1082 155 83 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 1329
Size: 87 32 1210
Fragments arranged by size:
1210 87 32
HindIII A'AGCT_T
Cuts at: 0 1226 1329
Size: 1226 103
KasI G'GCGC_C
Cuts at: 0 160 1329
Size: 160 1169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1329
Size: 897 432
MscI TGG'CCA
Cuts at: 0 1235 1329
Size: 1235 94
MslI CAynn'nnrTG
Cuts at: 0 1316 1329
Size: 1316 13
MspA1I CmG'CkG
Cuts at: 0 117 155 195 255 369 1048 1329
Size: 117 38 40 60 114 679 281
Fragments arranged by size:
679 281 117 114 60 40 38
NarI GG'CG_CC
Cuts at: 0 161 1329
Size: 161 1168
NcoI C'CATG_G
Cuts at: 0 59 740 1329
Size: 59 681 589
Fragments arranged by size:
681 589 59
NdeI CA'TA_TG
Cuts at: 0 1095 1317 1329
Size: 1095 222 12
Fragments arranged by size:
1095 222 12
NspI r_CATG'y
Cuts at: 0 685 1329
Size: 685 644
PasI CC'CwG_GG
Cuts at: 0 902 1329
Size: 902 427
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1329
Size: 741 588
PpuMI rG'GwC_Cy
Cuts at: 0 217 1329
Size: 217 1112
PspXI vC'TCGA_Gb
Cuts at: 0 989 1329
Size: 989 340
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 490 522 1329
Size: 490 32 807
Fragments arranged by size:
807 490 32
PstI C_TGCA'G
Cuts at: 0 563 1329
Size: 563 766
PvuII CAG'CTG
Cuts at: 0 369 1329
Size: 369 960
SacII CC_GC'GG
Cuts at: 0 156 1329
Size: 156 1173
SanDI GG'GwC_CC
Cuts at: 0 217 1329
Size: 217 1112
SapI GCTCTTCn'nnn_
Cuts at: 0 623 1329
Size: 623 706
SfcI C'TryA_G
Cuts at: 0 559 1329
Size: 559 770
SfoI GGC'GCC
Cuts at: 0 162 1329
Size: 162 1167
SmaI CCC'GGG
Cuts at: 0 133 166 1329
Size: 133 33 1163
Fragments arranged by size:
1163 133 33
SmlI C'TyrA_G
Cuts at: 0 989 1329
Size: 989 340
SrfI GCCC'GGGC
Cuts at: 0 166 1329
Size: 166 1163
SspI AAT'ATT
Cuts at: 0 1186 1329
Size: 1186 143
StyI C'CwwG_G
Cuts at: 0 59 620 740 1329
Size: 59 561 120 589
Fragments arranged by size:
589 561 120 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 1328 1329
Size: 270 1058 1
Fragments arranged by size:
1058 270 1
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 1329
Size: 839 32 458
Fragments arranged by size:
839 458 32
Tth111I GACn'n_nGTC
Cuts at: 0 99 1329
Size: 99 1230
XbaI T'CTAG_A
Cuts at: 0 804 1329
Size: 804 525
XhoI C'TCGA_G
Cuts at: 0 989 1329
Size: 989 340
XmaI C'CCGG_G
Cuts at: 0 131 164 1329
Size: 131 33 1165
Fragments arranged by size:
1165 131 33
Enzymes that do cut:
AcuI AgeI AlwNI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BglI BglII Bme1580I
BmgBI BplI BpmI BsaI BsaAI BsaHI BsaWI BseRI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI
BstYI BtgI DrdI EaeI EarI EciI Eco57MI EcoO109I
EcoRV FspI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NarI NcoI NdeI NspI PasI PflMI
PpuMI PspXI PsrI PstI PvuII SacII SanDI SapI
SfcI SfoI SmaI SmlI SrfI SspI StyI TaqII
TstI Tth111I XbaI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BbvCI BclI BfrBI BlpI BmrI BmtI
Bpu10I BpuEI BsaBI BsaXI BseYI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EcoICRI EcoNI EcoRI FalI FseI FspAI
HincII HpaI KpnI MfeI MluI NaeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI StuI SwaI TatI XcmI XmnI ZraI