Restriction map for
pFN21AB6601
(Linear) (Six-base) MAPSORT of: KIBB6601.seq Check: 9555 from: 1 to: 1221
KIBB6601.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AccI GT'mk_AC
Cuts at: 0 674 1221
Size: 674 547
Acc65I G'GTAC_C
Cuts at: 0 244 1221
Size: 244 977
AfeI AGC'GCT
Cuts at: 0 388 1221
Size: 388 833
AgeI A'CCGG_T
Cuts at: 0 241 1221
Size: 241 980
AlwNI CAG_nnn'CTG
Cuts at: 0 741 906 1221
Size: 741 165 315
Fragments arranged by size:
741 315 165
ApaI G_GGCC'C
Cuts at: 0 29 1221
Size: 29 1192
ApoI r'AATT_y
Cuts at: 0 1147 1221
Size: 1147 74
AvaI C'yCGr_G
Cuts at: 0 534 707 1053 1085 1221
Size: 534 173 346 32 136
Fragments arranged by size:
534 346 173 136 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 236 269 381 414 1221
Size: 236 33 112 33 807
Fragments arranged by size:
807 236 112 33 33
BanI G'GyrC_C
Cuts at: 0 244 562 1221
Size: 244 318 659
Fragments arranged by size:
659 318 244
BanII G_rGCy'C
Cuts at: 0 29 832 1221
Size: 29 803 389
Fragments arranged by size:
803 389 29
BbeI G_GCGC'C
Cuts at: 0 566 1221
Size: 566 655
BbsI GAAGACnn'nnnn_
Cuts at: 0 780 1221
Size: 780 441
BbvCI CC'TCA_GC
Cuts at: 0 967 1077 1221
Size: 967 110 144
Fragments arranged by size:
967 144 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1088 1221
Size: 1054 34 133
Fragments arranged by size:
1054 133 34
BglI GCCn_nnn'nGGC
Cuts at: 0 376 481 1221
Size: 376 105 740
Fragments arranged by size:
740 376 105
BlpI GC'TnA_GC
Cuts at: 0 734 1221
Size: 734 487
Bme1580I G_kGCm'C
Cuts at: 0 29 1221
Size: 29 1192
BmgBI CAC'GTC
Cuts at: 0 579 672 1221
Size: 579 93 549
Fragments arranged by size:
579 549 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1010 1221
Size: 1010 211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 940 1030 1221
Size: 76 864 90 191
Fragments arranged by size:
864 191 90 76
Bpu10I CC'TnA_GC
Cuts at: 0 136 967 1077 1221
Size: 136 831 110 144
Fragments arranged by size:
831 144 136 110
BsaHI Gr'CG_yC
Cuts at: 0 326 563 951 1221
Size: 326 237 388 270
Fragments arranged by size:
388 326 270 237
BsaWI w'CCGG_w
Cuts at: 0 241 1221
Size: 241 980
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 1221
Size: 163 30 1028
Fragments arranged by size:
1028 163 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 171 1221
Size: 73 98 1050
Fragments arranged by size:
1050 98 73
BseYI C'CCAG_C
Cuts at: 0 260 363 486 504 558 606 695 1003
Size: 260 103 123 18 54 48 89 308
Cuts at: 1003 1093 1162 1221
Size: 90 69 59
Fragments arranged by size:
308 260 123 103 90 89 69 59
54 48 18
BsiEI CG_ry'CG
Cuts at: 0 428 476 623 1221
Size: 428 48 147 598
Fragments arranged by size:
598 428 147 48
BsiHKAI G_wGCw'C
Cuts at: 0 947 1221
Size: 947 274
BsmBI CGTCTCn'nnnn_
Cuts at: 0 57 313 958 1221
Size: 57 256 645 263
Fragments arranged by size:
645 263 256 57
Bsp1286I G_dGCh'C
Cuts at: 0 29 832 947 1221
Size: 29 803 115 274
Fragments arranged by size:
803 274 115 29
BspHI T'CATG_A
Cuts at: 0 332 1221
Size: 332 889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 1221
Size: 134 1087
BsrFI r'CCGG_y
Cuts at: 0 241 1221
Size: 241 980
BssHII G'CGCG_C
Cuts at: 0 152 1221
Size: 152 1069
BssSI C'ACGA_G
Cuts at: 0 1070 1221
Size: 1070 151
BstEII G'GTnAC_C
Cuts at: 0 237 1221
Size: 237 984
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 1221
Size: 129 1092
BstYI r'GATC_y
Cuts at: 0 902 1221
Size: 902 319
BtgI C'CryG_G
Cuts at: 0 290 374 455 678 854 1221
Size: 290 84 81 223 176 367
Fragments arranged by size:
367 290 223 176 84 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 777 1221
Size: 777 444
BtsI GCAGTG_nn'
Cuts at: 0 1035 1113 1221
Size: 1035 78 108
Fragments arranged by size:
1035 108 78
DraIII CAC_nnn'GTG
Cuts at: 0 291 665 998 1221
Size: 291 374 333 223
Fragments arranged by size:
374 333 291 223
EaeI y'GGCC_r
Cuts at: 0 473 656 758 887 1221
Size: 473 183 102 129 334
Fragments arranged by size:
473 334 183 129 102
EagI C'GGCC_G
Cuts at: 0 473 1221
Size: 473 748
EarI CTCTTCn'nnn_
Cuts at: 0 280 601 1221
Size: 280 321 620
Fragments arranged by size:
620 321 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 922 1036 1221
Size: 922 114 185
Fragments arranged by size:
922 185 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 940 1030 1221
Size: 76 864 90 191
Fragments arranged by size:
864 191 90 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1193 1221
Size: 1193 28
EcoO109I rG'GnC_Cy
Cuts at: 0 25 26 539 1221
Size: 25 1 513 682
Fragments arranged by size:
682 513 25 1
HaeII r_GCGC'y
Cuts at: 0 390 441 487 566 1221
Size: 390 51 46 79 655
Fragments arranged by size:
655 390 79 51 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 717 749 974 1006 1017 1049
Size: 56 32 629 32 225 32 11 32
Cuts at: 1049 1221
Size: 172
Fragments arranged by size:
629 225 172 56 32 32 32 32
11
KasI G'GCGC_C
Cuts at: 0 562 1221
Size: 562 659
KpnI G_GTAC'C
Cuts at: 0 248 1221
Size: 248 973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1216 1221
Size: 146 1070 5
Fragments arranged by size:
1070 146 5
MscI TGG'CCA
Cuts at: 0 658 760 889 1221
Size: 658 102 129 332
Fragments arranged by size:
658 332 129 102
MslI CAynn'nnrTG
Cuts at: 0 253 754 1221
Size: 253 501 467
Fragments arranged by size:
501 467 253
MspA1I CmG'CkG
Cuts at: 0 357 843 1093 1221
Size: 357 486 250 128
Fragments arranged by size:
486 357 250 128
NarI GG'CG_CC
Cuts at: 0 563 1221
Size: 563 658
NcoI C'CATG_G
Cuts at: 0 374 1221
Size: 374 847
NotI GC'GGCC_GC
Cuts at: 0 473 1221
Size: 473 748
NspI r_CATG'y
Cuts at: 0 1134 1221
Size: 1134 87
PasI CC'CwG_GG
Cuts at: 0 21 914 1221
Size: 21 893 307
Fragments arranged by size:
893 307 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 906 1221
Size: 906 315
PfoI T'CCnGG_A
Cuts at: 0 905 1221
Size: 905 316
PpuMI rG'GwC_Cy
Cuts at: 0 539 1221
Size: 539 682
PspOMI G'GGCC_C
Cuts at: 0 25 1221
Size: 25 1196
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 913 945 1221
Size: 913 32 276
Fragments arranged by size:
913 276 32
PvuII CAG'CTG
Cuts at: 0 843 1093 1221
Size: 843 250 128
Fragments arranged by size:
843 250 128
SapI GCTCTTCn'nnn_
Cuts at: 0 280 601 1221
Size: 280 321 620
Fragments arranged by size:
620 321 280
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 376 1221
Size: 376 845
SfoI GGC'GCC
Cuts at: 0 564 1221
Size: 564 657
SmaI CCC'GGG
Cuts at: 0 536 709 1055 1221
Size: 536 173 346 166
Fragments arranged by size:
536 346 173 166
SphI G_CATG'C
Cuts at: 0 1134 1221
Size: 1134 87
StyI C'CwwG_G
Cuts at: 0 374 1221
Size: 374 847
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 906 1221
Size: 609 297 315
Fragments arranged by size:
609 315 297
TatI w'GTAC_w
Cuts at: 0 1013 1221
Size: 1013 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 1221
Size: 163 32 1026
Fragments arranged by size:
1026 163 32
Tth111I GACn'n_nGTC
Cuts at: 0 986 1221
Size: 986 235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 459 1221
Size: 459 762
XmaI C'CCGG_G
Cuts at: 0 534 707 1053 1221
Size: 534 173 346 168
Fragments arranged by size:
534 346 173 168
Enzymes that do cut:
AccI Acc65I AfeI AgeI AlwNI ApaI ApoI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrFI BssHII BssSI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NotI NspI PasI PflMI
PfoI PpuMI PspOMI PsrI PvuII SapI SfiI SfoI
SmaI SphI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AflIII AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI
BciVI BclI BfrBI BglII BmrI BmtI BpuEI BsaI
BsaAI BsaBI BsgI BsiWI BsmI BspEI BsrBI BsrDI
BsrGI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI
XhoI XmnI ZraI