(Linear) (Six-base) MAPSORT of: KIBB6536.seq  Check: 2504  from: 1  to: 1170



KIBB6536.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:22  ..

AccI GT'mk_AC

Cuts at:      0     690    1170 
   Size:        690     480
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1021    1170 
   Size:       1021     149
 
AfeI AGC'GCT

Cuts at:      0    1090    1170 
   Size:       1090      80
 
AflIII A'CryG_T

Cuts at:      0     873    1170 
   Size:        873     297
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     850    1170 
   Size:        301     549     320

  Fragments arranged by size: 

                 549     320     301
 
ApoI r'AATT_y

Cuts at:      0     544    1170 
   Size:        544     626
 
BanI G'GyrC_C

Cuts at:      0     626    1170 
   Size:        626     544
 
BanII G_rGCy'C

Cuts at:      0     951    1170 
   Size:        951     219
 
BbvCI CC'TCA_GC

Cuts at:      0     990    1170 
   Size:        990     180
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1082    1170 
   Size:       1082      88
 
BclI T'GATC_A

Cuts at:      0     612    1170 
   Size:        612     558
 
BglII A'GATC_T

Cuts at:      0     706    1170 
   Size:        706     464
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     389     550    1170 
   Size:        389     161     620

  Fragments arranged by size: 

                 620     389     161
 
Bpu10I CC'TnA_GC

Cuts at:      0     990    1170 
   Size:        990     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771    1170 
   Size:        771     399
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     753     783    1170 
   Size:        753      30     387

  Fragments arranged by size: 

                 753     387      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     774    1170 
   Size:        774     396
 
BseYI C'CCAG_C

Cuts at:      0     944    1170 
   Size:        944     226
 
Bsp1286I G_dGCh'C

Cuts at:      0     951    1170 
   Size:        951     219
 
BsrGI T'GTAC_A

Cuts at:      0     212     292    1170 
   Size:        212      80     878

  Fragments arranged by size: 

                 878     212      80
 
BssSI C'ACGA_G

Cuts at:      0      41    1170 
   Size:         41    1129
 
BstBI TT'CG_AA

Cuts at:      0     233    1170 
   Size:        233     937
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     912    1170 
   Size:        912     258
 
BstYI r'GATC_y

Cuts at:      0     706    1170 
   Size:        706     464
 
ClaI AT'CG_AT

Cuts at:      0     333    1170 
   Size:        333     837
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     651     686    1170 
   Size:        651      35     484

  Fragments arranged by size: 

                 651     484      35
 
EaeI y'GGCC_r

Cuts at:      0     170     410     914    1170 
   Size:        170     240     504     256

  Fragments arranged by size: 

                 504     256     240     170
 
EarI CTCTTCn'nnn_

Cuts at:      0     663     863    1170 
   Size:        663     200     307

  Fragments arranged by size: 

                 663     307     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1021    1170 
   Size:       1021     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1000    1170 
   Size:       1000     170
 
EcoRI G'AATT_C

Cuts at:      0     544    1170 
   Size:        544     626
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1122    1170 
   Size:       1122      48
 
HaeII r_GCGC'y

Cuts at:      0    1092    1170 
   Size:       1092      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     384     416    1170 
   Size:        384      32     754

  Fragments arranged by size: 

                 754     384      32
 
MfeI C'AATT_G

Cuts at:      0     812     968    1170 
   Size:        812     156     202

  Fragments arranged by size: 

                 812     202     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1170 
   Size:        686     484
 
MscI TGG'CCA

Cuts at:      0     172     916    1170 
   Size:        172     744     254

  Fragments arranged by size: 

                 744     254     172
 
NspI r_CATG'y

Cuts at:      0     877    1170 
   Size:        877     293
 
PciI A'CATG_T

Cuts at:      0     873    1170 
   Size:        873     297
 
PfoI T'CCnGG_A

Cuts at:      0     639    1170 
   Size:        639     531
 
PpuMI rG'GwC_Cy

Cuts at:      0    1000    1170 
   Size:       1000     170
 
PshAI GACnn'nnGTC

Cuts at:      0     652    1170 
   Size:        652     518
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     360     392    1170 
   Size:        360      32     778

  Fragments arranged by size: 

                 778     360      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0     863    1170 
   Size:        863     307
 
SfcI C'TryA_G

Cuts at:      0     851    1170 
   Size:        851     319
 
SmlI C'TyrA_G

Cuts at:      0     786    1170 
   Size:        786     384
 
SspI AAT'ATT

Cuts at:      0     588    1115    1170 
   Size:        588     527      55

  Fragments arranged by size: 

                 588     527      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181     408    1145    1170 
   Size:        181     227     737      25

  Fragments arranged by size: 

                 737     227     181      25
 
TatI w'GTAC_w

Cuts at:      0     212     292    1170 
   Size:        212      80     878

  Fragments arranged by size: 

                 878     212      80
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     637    1170 
   Size:        637     533
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII    AlwNI     ApoI     BanI    BanII
    BbvCI    BciVI     BclI    BglII     BmrI   Bpu10I    BpuEI    BsaXI
    BseRI    BseYI Bsp1286I    BsrGI    BssSI    BstBI    BstXI    BstYI
     ClaI    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI
    HaeII    Hin4I     MfeI     MmeI     MscI     NspI     PciI     PfoI
    PpuMI    PshAI     PsrI     SapI     SfcI     SmlI     SspI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI