Restriction map for
pFN21AB6530
(Linear) (Six-base) MAPSORT of: KIBB6530.seq Check: 1601 from: 1 to: 837
KIBB6530.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 124 837
Size: 124 713
Acc65I G'GTAC_C
Cuts at: 0 331 837
Size: 331 506
AfeI AGC'GCT
Cuts at: 0 10 595 837
Size: 10 585 242
Fragments arranged by size:
585 242 10
AlwNI CAG_nnn'CTG
Cuts at: 0 362 837
Size: 362 475
ApaLI G'TGCA_C
Cuts at: 0 561 669 837
Size: 561 108 168
Fragments arranged by size:
561 168 108
ApoI r'AATT_y
Cuts at: 0 441 837
Size: 441 396
BanI G'GyrC_C
Cuts at: 0 331 837
Size: 331 506
BanII G_rGCy'C
Cuts at: 0 76 837
Size: 76 761
BbsI GAAGACnn'nnnn_
Cuts at: 0 322 837
Size: 322 515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 632 837
Size: 598 34 205
Fragments arranged by size:
598 205 34
Bme1580I G_kGCm'C
Cuts at: 0 455 565 673 817 837
Size: 455 110 108 144 20
Fragments arranged by size:
455 144 110 108 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 364 837
Size: 91 273 473
Fragments arranged by size:
473 273 91
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 136 837
Size: 82 54 701
Fragments arranged by size:
701 82 54
BseYI C'CCAG_C
Cuts at: 0 664 837
Size: 664 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 837
Size: 231 606
BsiHKAI G_wGCw'C
Cuts at: 0 565 673 763 837
Size: 565 108 90 74
Fragments arranged by size:
565 108 90 74
BsmI GAATG_Cn'
Cuts at: 0 313 837
Size: 313 524
Bsp1286I G_dGCh'C
Cuts at: 0 76 455 565 673 763 817 837
Size: 76 379 110 108 90 54 20
Fragments arranged by size:
379 110 108 90 76 54 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 382 837
Size: 124 258 455
Fragments arranged by size:
455 258 124
BsrDI GCAATG_nn'
Cuts at: 0 24 837
Size: 24 813
BsrFI r'CCGG_y
Cuts at: 0 173 837
Size: 173 664
BsrGI T'GTAC_A
Cuts at: 0 265 837
Size: 265 572
BstAPI GCAn_nnn'nTGC
Cuts at: 0 569 837
Size: 569 268
BtgI C'CryG_G
Cuts at: 0 509 837
Size: 509 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 623 837
Size: 623 214
BtsI GCAGTG_nn'
Cuts at: 0 198 214 487 733 837
Size: 198 16 273 246 104
Fragments arranged by size:
273 246 198 104 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 837
Size: 64 773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 570 837
Size: 570 267
HaeII r_GCGC'y
Cuts at: 0 12 597 837
Size: 12 585 240
Fragments arranged by size:
585 240 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 837
Size: 277 32 528
Fragments arranged by size:
528 277 32
KpnI G_GTAC'C
Cuts at: 0 335 837
Size: 335 502
MfeI C'AATT_G
Cuts at: 0 404 837
Size: 404 433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 837
Size: 305 532
MslI CAynn'nnrTG
Cuts at: 0 182 297 837
Size: 182 115 540
Fragments arranged by size:
540 182 115
MspA1I CmG'CkG
Cuts at: 0 258 362 392 582 686 837
Size: 258 104 30 190 104 151
Fragments arranged by size:
258 190 151 104 104 30
NruI TCG'CGA
Cuts at: 0 608 837
Size: 608 229
NspI r_CATG'y
Cuts at: 0 475 837
Size: 475 362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 837
Size: 88 32 717
Fragments arranged by size:
717 88 32
PstI C_TGCA'G
Cuts at: 0 574 837
Size: 574 263
PvuII CAG'CTG
Cuts at: 0 258 362 582 837
Size: 258 104 220 255
Fragments arranged by size:
258 255 220 104
SbfI CC_TGCA'GG
Cuts at: 0 574 837
Size: 574 263
ScaI AGT'ACT
Cuts at: 0 289 837
Size: 289 548
SfcI C'TryA_G
Cuts at: 0 570 837
Size: 570 267
StyI C'CwwG_G
Cuts at: 0 245 837
Size: 245 592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 656 837
Size: 656 181
TatI w'GTAC_w
Cuts at: 0 53 265 287 347 837
Size: 53 212 22 60 490
Fragments arranged by size:
490 212 60 53 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 712 744 837
Size: 712 32 93
Fragments arranged by size:
712 93 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 403 837
Size: 403 434
XmnI GAAnn'nnTTC
Cuts at: 0 634 837
Size: 634 203
Enzymes that do cut:
AarI Acc65I AfeI AlwNI ApaLI ApoI BanI BanII
BbsI BcgI Bme1580I BsaI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BtgI
BtgZI BtsI EcoNI FalI HaeII Hin4I KpnI MfeI
MmeI MslI MspA1I NruI NspI PpiI PstI PvuII
SbfI ScaI SfcI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI