Restriction map for
pFN21AB6526
(Linear) (Six-base) MAPSORT of: KIBB6526.seq Check: 932 from: 1 to: 1077
KIBB6526.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1077
Size: 410 667
AflII C'TTAA_G
Cuts at: 0 868 1077
Size: 868 209
AflIII A'CryG_T
Cuts at: 0 160 1077
Size: 160 917
AlwNI CAG_nnn'CTG
Cuts at: 0 237 388 529 1077
Size: 237 151 141 548
Fragments arranged by size:
548 237 151 141
ApaI G_GGCC'C
Cuts at: 0 278 1077
Size: 278 799
ApoI r'AATT_y
Cuts at: 0 760 1077
Size: 760 317
AvrII C'CTAG_G
Cuts at: 0 993 1077
Size: 993 84
BamHI G'GATC_C
Cuts at: 0 938 1077
Size: 938 139
BanII G_rGCy'C
Cuts at: 0 278 1077
Size: 278 799
BbsI GAAGACnn'nnnn_
Cuts at: 0 25 1077
Size: 25 1052
BbvCI CC'TCA_GC
Cuts at: 0 537 1077
Size: 537 540
BglII A'GATC_T
Cuts at: 0 219 1077
Size: 219 858
BlpI GC'TnA_GC
Cuts at: 0 572 1077
Size: 572 505
Bme1580I G_kGCm'C
Cuts at: 0 278 1077
Size: 278 799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 480 1077
Size: 480 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1077
Size: 571 506
Bpu10I CC'TnA_GC
Cuts at: 0 537 1077
Size: 537 540
BsaI GGTCTCn'nnnn_
Cuts at: 0 992 1077
Size: 992 85
BsaWI w'CCGG_w
Cuts at: 0 494 516 1077
Size: 494 22 561
Fragments arranged by size:
561 494 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 1077
Size: 469 608
BseYI C'CCAG_C
Cuts at: 0 249 933 1077
Size: 249 684 144
Fragments arranged by size:
684 249 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 289 642 1077
Size: 250 39 353 435
Fragments arranged by size:
435 353 250 39
Bsp1286I G_dGCh'C
Cuts at: 0 278 1077
Size: 278 799
BspEI T'CCGG_A
Cuts at: 0 494 1077
Size: 494 583
BspHI T'CATG_A
Cuts at: 0 1062 1077
Size: 1062 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 365 1077
Size: 365 712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1077
Size: 237 840
BstEII G'GTnAC_C
Cuts at: 0 348 1077
Size: 348 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 1077
Size: 51 1026
BstYI r'GATC_y
Cuts at: 0 219 938 1077
Size: 219 719 139
Fragments arranged by size:
719 219 139
Bsu36I CC'TnA_GG
Cuts at: 0 269 1077
Size: 269 808
BtgI C'CryG_G
Cuts at: 0 618 1077
Size: 618 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1028 1077
Size: 1028 49
BtsI GCAGTG_nn'
Cuts at: 0 373 1077
Size: 373 704
DraI TTT'AAA
Cuts at: 0 132 1077
Size: 132 945
DrdI GACnn_nn'nnGTC
Cuts at: 0 106 1077
Size: 106 971
EaeI y'GGCC_r
Cuts at: 0 177 1077
Size: 177 900
EarI CTCTTCn'nnn_
Cuts at: 0 424 1077
Size: 424 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 541 1077
Size: 541 536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 571 1077
Size: 410 161 506
Fragments arranged by size:
506 410 161
EcoO109I rG'GnC_Cy
Cuts at: 0 274 275 385 526 846 1077
Size: 274 1 110 141 320 231
Fragments arranged by size:
320 274 231 141 110 1
EcoRI G'AATT_C
Cuts at: 0 760 1077
Size: 760 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 614 646 1077
Size: 614 32 431
Fragments arranged by size:
614 431 32
HincII GTy'rAC
Cuts at: 0 158 696 966 1077
Size: 158 538 270 111
Fragments arranged by size:
538 270 158 111
MspA1I CmG'CkG
Cuts at: 0 253 833 1077
Size: 253 580 244
Fragments arranged by size:
580 253 244
NspI r_CATG'y
Cuts at: 0 164 1077
Size: 164 913
PciI A'CATG_T
Cuts at: 0 160 1077
Size: 160 917
PpuMI rG'GwC_Cy
Cuts at: 0 385 1077
Size: 385 692
PspOMI G'GGCC_C
Cuts at: 0 274 1077
Size: 274 803
PstI C_TGCA'G
Cuts at: 0 344 380 1077
Size: 344 36 697
Fragments arranged by size:
697 344 36
PvuII CAG'CTG
Cuts at: 0 253 833 1077
Size: 253 580 244
Fragments arranged by size:
580 253 244
ScaI AGT'ACT
Cuts at: 0 127 1077
Size: 127 950
SfcI C'TryA_G
Cuts at: 0 289 340 376 773 1077
Size: 289 51 36 397 304
Fragments arranged by size:
397 304 289 51 36
SmlI C'TyrA_G
Cuts at: 0 868 1077
Size: 868 209
StuI AGG'CCT
Cuts at: 0 592 1077
Size: 592 485
StyI C'CwwG_G
Cuts at: 0 5 993 1034 1077
Size: 5 988 41 43
Fragments arranged by size:
988 43 41 5
TatI w'GTAC_w
Cuts at: 0 125 501 734 1077
Size: 125 376 233 343
Fragments arranged by size:
376 343 233 125
XmnI GAAnn'nnTTC
Cuts at: 0 433 1077
Size: 433 644
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApaI ApoI AvrII BamHI
BanII BbsI BbvCI BglII BlpI Bme1580I BplI BpmI
Bpu10I BsaI BsaWI BseRI BseYI BsgI Bsp1286I BspEI
BspHI BspMI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EarI EciI Eco57MI
EcoO109I EcoRI Hin4I HincII MspA1I NspI PciI PpuMI
PspOMI PstI PvuII ScaI SfcI SmlI StuI StyI
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I ClaI CspCI DraIII
EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI