Restriction map for
pFN21AB6508
(Linear) (Six-base) MAPSORT of: KIBB6508.seq Check: 445 from: 1 to: 1152
KIBB6508.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
Acc65I G'GTAC_C
Cuts at: 0 299 1152
Size: 299 853
AclI AA'CG_TT
Cuts at: 0 218 1152
Size: 218 934
AflIII A'CryG_T
Cuts at: 0 274 750 1152
Size: 274 476 402
Fragments arranged by size:
476 402 274
AlwNI CAG_nnn'CTG
Cuts at: 0 914 1152
Size: 914 238
AvaI C'yCGr_G
Cuts at: 0 108 1152
Size: 108 1044
BamHI G'GATC_C
Cuts at: 0 113 1152
Size: 113 1039
BanI G'GyrC_C
Cuts at: 0 299 1152
Size: 299 853
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 642 1152
Size: 375 267 510
Fragments arranged by size:
510 375 267
BclI T'GATC_A
Cuts at: 0 182 1031 1152
Size: 182 849 121
Fragments arranged by size:
849 182 121
BglI GCCn_nnn'nGGC
Cuts at: 0 167 1025 1152
Size: 167 858 127
Fragments arranged by size:
858 167 127
BglII A'GATC_T
Cuts at: 0 188 467 1152
Size: 188 279 685
Fragments arranged by size:
685 279 188
BlpI GC'TnA_GC
Cuts at: 0 973 1152
Size: 973 179
Bme1580I G_kGCm'C
Cuts at: 0 591 921 1152
Size: 591 330 231
Fragments arranged by size:
591 330 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 277 1152
Size: 277 875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 266 1152
Size: 48 218 886
Fragments arranged by size:
886 218 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1152
Size: 1066 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 248 1046 1152
Size: 248 798 106
Fragments arranged by size:
798 248 106
BsaBI GATnn'nnATC
Cuts at: 0 187 466 1152
Size: 187 279 686
Fragments arranged by size:
686 279 187
BsaWI w'CCGG_w
Cuts at: 0 1037 1152
Size: 1037 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 264 294 1152
Size: 264 30 858
Fragments arranged by size:
858 264 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 1152
Size: 253 899
BsiHKAI G_wGCw'C
Cuts at: 0 282 1152
Size: 282 870
Bsp1286I G_dGCh'C
Cuts at: 0 282 591 921 1152
Size: 282 309 330 231
Fragments arranged by size:
330 309 282 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 287 977 1075 1152
Size: 266 21 690 98 77
Fragments arranged by size:
690 266 98 77 21
BsrBI CCG'CTC
Cuts at: 0 860 1143 1152
Size: 860 283 9
Fragments arranged by size:
860 283 9
BsrDI GCAATG_nn'
Cuts at: 0 695 1152
Size: 695 457
BsrGI T'GTAC_A
Cuts at: 0 196 1152
Size: 196 956
BssSI C'ACGA_G
Cuts at: 0 1138 1152
Size: 1138 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1152
Size: 510 642
BstYI r'GATC_y
Cuts at: 0 78 113 188 467 1152
Size: 78 35 75 279 685
Fragments arranged by size:
685 279 78 75 35
BtgI C'CryG_G
Cuts at: 0 734 1100 1152
Size: 734 366 52
Fragments arranged by size:
734 366 52
BtsI GCAGTG_nn'
Cuts at: 0 51 1152
Size: 51 1101
EaeI y'GGCC_r
Cuts at: 0 430 512 1103 1152
Size: 430 82 591 49
Fragments arranged by size:
591 430 82 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 266 1152
Size: 48 218 886
Fragments arranged by size:
886 218 48
EcoRV GAT'ATC
Cuts at: 0 441 1152
Size: 441 711
HaeII r_GCGC'y
Cuts at: 0 400 1152
Size: 400 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1152
Size: 85 32 1035
Fragments arranged by size:
1035 85 32
KpnI G_GTAC'C
Cuts at: 0 303 1152
Size: 303 849
MscI TGG'CCA
Cuts at: 0 514 1105 1152
Size: 514 591 47
Fragments arranged by size:
591 514 47
MslI CAynn'nnrTG
Cuts at: 0 502 605 889 1152
Size: 502 103 284 263
Fragments arranged by size:
502 284 263 103
MspA1I CmG'CkG
Cuts at: 0 1008 1152
Size: 1008 144
NspI r_CATG'y
Cuts at: 0 754 1152
Size: 754 398
PciI A'CATG_T
Cuts at: 0 750 1152
Size: 750 402
PflMI CCAn_nnn'nTGG
Cuts at: 0 633 1152
Size: 633 519
PfoI T'CCnGG_A
Cuts at: 0 772 1152
Size: 772 380
PstI C_TGCA'G
Cuts at: 0 135 1152
Size: 135 1017
PvuII CAG'CTG
Cuts at: 0 1008 1152
Size: 1008 144
SfcI C'TryA_G
Cuts at: 0 131 1152
Size: 131 1021
SmaI CCC'GGG
Cuts at: 0 110 1152
Size: 110 1042
SmlI C'TyrA_G
Cuts at: 0 1081 1152
Size: 1081 71
StuI AGG'CCT
Cuts at: 0 161 448 1152
Size: 161 287 704
Fragments arranged by size:
704 287 161
TatI w'GTAC_w
Cuts at: 0 196 1060 1152
Size: 196 864 92
Fragments arranged by size:
864 196 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 300 795 1098 1152
Size: 246 54 495 303 54
Fragments arranged by size:
495 303 246 54 54
XmaI C'CCGG_G
Cuts at: 0 108 1152
Size: 108 1044
XmnI GAAnn'nnTTC
Cuts at: 0 994 1152
Size: 994 158
Enzymes that do cut:
Acc65I AclI AflIII AlwNI AvaI BamHI BanI BbsI
BclI BglI BglII BlpI Bme1580I BplI BpmI BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrGI BssSI BstXI BstYI BtgI BtsI
EaeI Eco57MI EcoRV HaeII Hin4I KpnI MscI MslI
MspA1I NspI PciI PflMI PfoI PstI PvuII SfcI
SmaI SmlI StuI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BfrBI BmgBI BmrI BmtI Bpu10I BsaAI BsaHI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TstI Tth111I XbaI XhoI ZraI