Restriction map for
pFN21AB6481
(Linear) (Six-base) MAPSORT of: KIBB6481.seq Check: 6957 from: 1 to: 933
KIBB6481.orf
With 182 enzymes: SgfI *
July 3, 2012 16:22 ..
AgeI A'CCGG_T
Cuts at: 0 359 933
Size: 359 574
AhdI GACnn_n'nnGTC
Cuts at: 0 348 933
Size: 348 585
AleI CACnn'nnGTG
Cuts at: 0 859 933
Size: 859 74
AlwNI CAG_nnn'CTG
Cuts at: 0 213 933
Size: 213 720
ApaI G_GGCC'C
Cuts at: 0 502 933
Size: 502 431
AvaI C'yCGr_G
Cuts at: 0 679 933
Size: 679 254
BanI G'GyrC_C
Cuts at: 0 356 367 933
Size: 356 11 566
Fragments arranged by size:
566 356 11
BanII G_rGCy'C
Cuts at: 0 502 680 933
Size: 502 178 253
Fragments arranged by size:
502 253 178
BbeI G_GCGC'C
Cuts at: 0 371 933
Size: 371 562
BglI GCCn_nnn'nGGC
Cuts at: 0 544 933
Size: 544 389
Bme1580I G_kGCm'C
Cuts at: 0 502 688 773 933
Size: 502 186 85 160
Fragments arranged by size:
502 186 160 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 866 933
Size: 866 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 879 933
Size: 799 80 54
Fragments arranged by size:
799 80 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 335 933
Size: 121 214 598
Fragments arranged by size:
598 214 121
BsaAI yAC'GTr
Cuts at: 0 201 666 933
Size: 201 465 267
Fragments arranged by size:
465 267 201
BsaHI Gr'CG_yC
Cuts at: 0 10 368 425 933
Size: 10 358 57 508
Fragments arranged by size:
508 358 57 10
BsaWI w'CCGG_w
Cuts at: 0 120 359 933
Size: 120 239 574
Fragments arranged by size:
574 239 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 273 303 655 685 933
Size: 31 30 212 30 352 30 248
Fragments arranged by size:
352 248 212 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 568 673 861 864 882 933
Size: 127 441 105 188 3 18 51
Fragments arranged by size:
441 188 127 105 51 18 3
BseYI C'CCAG_C
Cuts at: 0 490 711 904 933
Size: 490 221 193 29
Fragments arranged by size:
490 221 193 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 933
Size: 499 434
BsiEI CG_ry'CG
Cuts at: 0 414 933
Size: 414 519
BsiHKAI G_wGCw'C
Cuts at: 0 139 200 665 933
Size: 139 61 465 268
Fragments arranged by size:
465 268 139 61
Bsp1286I G_dGCh'C
Cuts at: 0 139 200 502 665 680 688 773 933
Size: 139 61 302 163 15 8 85 160
Fragments arranged by size:
302 163 160 139 85 61 15 8
BspEI T'CCGG_A
Cuts at: 0 120 933
Size: 120 813
BsrFI r'CCGG_y
Cuts at: 0 182 329 359 409 413 609 933
Size: 182 147 30 50 4 196 324
Fragments arranged by size:
324 196 182 147 50 30 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 933
Size: 555 378
BstYI r'GATC_y
Cuts at: 0 275 933
Size: 275 658
BtgI C'CryG_G
Cuts at: 0 50 933
Size: 50 883
BtsI GCAGTG_nn'
Cuts at: 0 39 933
Size: 39 894
EaeI y'GGCC_r
Cuts at: 0 2 184 411 833 933
Size: 2 182 227 422 100
Fragments arranged by size:
422 227 182 100 2
EagI C'GGCC_G
Cuts at: 0 411 933
Size: 411 522
EarI CTCTTCn'nnn_
Cuts at: 0 119 933
Size: 119 814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 879 933
Size: 799 80 54
Fragments arranged by size:
799 80 54
EcoO109I rG'GnC_Cy
Cuts at: 0 318 499 601 633 933
Size: 318 181 102 32 300
Fragments arranged by size:
318 300 181 102 32
HaeII r_GCGC'y
Cuts at: 0 371 933
Size: 371 562
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 330 334 362 366 933
Size: 330 4 28 4 567
Fragments arranged by size:
567 330 28 4 4
HincII GTy'rAC
Cuts at: 0 462 933
Size: 462 471
KasI G'GCGC_C
Cuts at: 0 367 933
Size: 367 566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 933
Size: 173 760
MslI CAynn'nnrTG
Cuts at: 0 859 933
Size: 859 74
MspA1I CmG'CkG
Cuts at: 0 732 794 933
Size: 732 62 139
Fragments arranged by size:
732 139 62
NaeI GCC'GGC
Cuts at: 0 184 331 415 933
Size: 184 147 84 518
Fragments arranged by size:
518 184 147 84
NarI GG'CG_CC
Cuts at: 0 368 933
Size: 368 565
NgoMIV G'CCGG_C
Cuts at: 0 182 329 413 933
Size: 182 147 84 520
Fragments arranged by size:
520 182 147 84
PasI CC'CwG_GG
Cuts at: 0 503 933
Size: 503 430
PfoI T'CCnGG_A
Cuts at: 0 21 933
Size: 21 912
PmlI CAC'GTG
Cuts at: 0 201 933
Size: 201 732
PpuMI rG'GwC_Cy
Cuts at: 0 318 933
Size: 318 615
PspOMI G'GGCC_C
Cuts at: 0 498 933
Size: 498 435
PspXI vC'TCGA_Gb
Cuts at: 0 679 933
Size: 679 254
PvuII CAG'CTG
Cuts at: 0 732 794 933
Size: 732 62 139
Fragments arranged by size:
732 139 62
SanDI GG'GwC_CC
Cuts at: 0 318 933
Size: 318 615
SfoI GGC'GCC
Cuts at: 0 369 933
Size: 369 564
SmlI C'TyrA_G
Cuts at: 0 679 933
Size: 679 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 703 933
Size: 703 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 273 305 655 687 933
Size: 31 32 210 32 350 32 246
Fragments arranged by size:
350 246 210 32 32 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 933
Size: 492 441
XhoI C'TCGA_G
Cuts at: 0 679 933
Size: 679 254
Enzymes that do cut:
AgeI AhdI AleI AlwNI ApaI AvaI BanI BanII
BbeI BglI Bme1580I BmrI BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BsrFI BstXI BstYI BtgI BtsI EaeI EagI
EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV PasI PfoI PmlI
PpuMI PspOMI PspXI PvuII SanDI SfoI SmlI TaqII
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI Tth111I XbaI XmaI XmnI
ZraI