(Linear) (Six-base) MAPSORT of: KIBB6456.seq  Check: 6050  from: 1  to: 1053



KIBB6456.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AccI GT'mk_AC

Cuts at:      0     206    1053 
   Size:        206     847
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     722    1053 
   Size:         41     681     331

  Fragments arranged by size: 

                 681     331      41
 
AfeI AGC'GCT

Cuts at:      0     328    1053 
   Size:        328     725
 
ApoI r'AATT_y

Cuts at:      0     315     380     457     963    1053 
   Size:        315      65      77     506      90

  Fragments arranged by size: 

                 506     315      90      77      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1023    1053 
   Size:       1023      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1053 
   Size:        364     689
 
BsiEI CG_ry'CG

Cuts at:      0      29    1053 
   Size:         29    1024
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     336    1053 
   Size:        336     717
 
BsrFI r'CCGG_y

Cuts at:      0     119    1053 
   Size:        119     934
 
BsrGI T'GTAC_A

Cuts at:      0     410    1053 
   Size:        410     643
 
BstYI r'GATC_y

Cuts at:      0     219     441     598    1053 
   Size:        219     222     157     455

  Fragments arranged by size: 

                 455     222     219     157
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     534    1053 
   Size:        534     519
 
EaeI y'GGCC_r

Cuts at:      0      26    1053 
   Size:         26    1027
 
EagI C'GGCC_G

Cuts at:      0      26    1053 
   Size:         26    1027
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     194     622    1053 
   Size:         86     108     428     431

  Fragments arranged by size: 

                 431     428     108      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     722    1053 
   Size:         41     681     331

  Fragments arranged by size: 

                 681     331      41
 
HaeII r_GCGC'y

Cuts at:      0     330    1053 
   Size:        330     723
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      37      66      69     523     555    1053 
   Size:         34       3      29       3     454      32     498

  Fragments arranged by size: 

                 498     454      34      32      29       3       3
 
HincII GTy'rAC

Cuts at:      0     207    1053 
   Size:        207     846
 
HindIII A'AGCT_T

Cuts at:      0     238    1053 
   Size:        238     815
 
MfeI C'AATT_G

Cuts at:      0     473    1053 
   Size:        473     580
 
MslI CAynn'nnrTG

Cuts at:      0     826     841    1053 
   Size:        826      15     212

  Fragments arranged by size: 

                 826     212      15
 
SalI G'TCGA_C

Cuts at:      0     205    1053 
   Size:        205     848
 
SapI GCTCTTCn'nnn_

Cuts at:      0      86    1053 
   Size:         86     967
 
ScaI AGT'ACT

Cuts at:      0     259    1053 
   Size:        259     794
 
SfcI C'TryA_G

Cuts at:      0     575    1053 
   Size:        575     478
 
StyI C'CwwG_G

Cuts at:      0    1037    1053 
   Size:       1037      16
 
TatI w'GTAC_w

Cuts at:      0     257     410     851    1053 
   Size:        257     153     441     202

  Fragments arranged by size: 

                 441     257     202     153
 
XmnI GAAnn'nnTTC

Cuts at:      0     111     259     686    1053 
   Size:        111     148     427     367

  Fragments arranged by size: 

                 427     367     148     111
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     ApoI     BbsI     BsgI    BsiEI    BspMI
    BsrFI    BsrGI    BstYI    BtgZI     EaeI     EagI     EarI  Eco57MI
    HaeII    Hin4I   HincII  HindIII     MfeI     MslI     SalI     SapI
     ScaI     SfcI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI