Restriction map for
pFN21AB6451
(Linear) (Six-base) MAPSORT of: KIBB6451.seq Check: 3147 from: 1 to: 954
KIBB6451.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AccI GT'mk_AC
Cuts at: 0 639 954
Size: 639 315
Acc65I G'GTAC_C
Cuts at: 0 246 954
Size: 246 708
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 954
Size: 553 401
AlwNI CAG_nnn'CTG
Cuts at: 0 166 324 820 954
Size: 166 158 496 134
Fragments arranged by size:
496 166 158 134
ApaI G_GGCC'C
Cuts at: 0 125 954
Size: 125 829
ApoI r'AATT_y
Cuts at: 0 773 794 954
Size: 773 21 160
Fragments arranged by size:
773 160 21
BanI G'GyrC_C
Cuts at: 0 246 629 883 954
Size: 246 383 254 71
Fragments arranged by size:
383 254 246 71
BanII G_rGCy'C
Cuts at: 0 125 954
Size: 125 829
BciVI GTATCCnnnnn_n'
Cuts at: 0 71 83 954
Size: 71 12 871
Fragments arranged by size:
871 71 12
BglI GCCn_nnn'nGGC
Cuts at: 0 176 954
Size: 176 778
Bme1580I G_kGCm'C
Cuts at: 0 125 954
Size: 125 829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 544 954
Size: 83 461 410
Fragments arranged by size:
461 410 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 954
Size: 504 450
BsaI GGTCTCn'nnnn_
Cuts at: 0 522 954
Size: 522 432
BsaBI GATnn'nnATC
Cuts at: 0 580 954
Size: 580 374
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 56 581 954
Size: 56 525 373
Fragments arranged by size:
525 373 56
BseYI C'CCAG_C
Cuts at: 0 171 954
Size: 171 783
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 954
Size: 729 225
BsmI GAATG_Cn'
Cuts at: 0 30 85 954
Size: 30 55 869
Fragments arranged by size:
869 55 30
Bsp1286I G_dGCh'C
Cuts at: 0 125 954
Size: 125 829
BsrFI r'CCGG_y
Cuts at: 0 151 954
Size: 151 803
BtgI C'CryG_G
Cuts at: 0 250 954
Size: 250 704
EaeI y'GGCC_r
Cuts at: 0 153 954
Size: 153 801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 544 553 954
Size: 83 461 9 401
Fragments arranged by size:
461 401 83 9
EcoO109I rG'GnC_Cy
Cuts at: 0 94 122 163 954
Size: 94 28 41 791
Fragments arranged by size:
791 94 41 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 954
Size: 417 537
HaeII r_GCGC'y
Cuts at: 0 265 954
Size: 265 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 729 759 761 954
Size: 727 2 30 2 193
Fragments arranged by size:
727 193 30 2 2
KpnI G_GTAC'C
Cuts at: 0 250 954
Size: 250 704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 954
Size: 213 741
MslI CAynn'nnrTG
Cuts at: 0 537 954
Size: 537 417
MspA1I CmG'CkG
Cuts at: 0 175 427 548 713 954
Size: 175 252 121 165 241
Fragments arranged by size:
252 241 175 165 121
NaeI GCC'GGC
Cuts at: 0 153 954
Size: 153 801
NcoI C'CATG_G
Cuts at: 0 250 954
Size: 250 704
NgoMIV G'CCGG_C
Cuts at: 0 151 954
Size: 151 803
PasI CC'CwG_GG
Cuts at: 0 118 954
Size: 118 836
PflMI CCAn_nnn'nTGG
Cuts at: 0 785 954
Size: 785 169
PfoI T'CCnGG_A
Cuts at: 0 99 954
Size: 99 855
PpuMI rG'GwC_Cy
Cuts at: 0 94 163 954
Size: 94 69 791
Fragments arranged by size:
791 94 69
PspOMI G'GGCC_C
Cuts at: 0 121 954
Size: 121 833
PstI C_TGCA'G
Cuts at: 0 243 954
Size: 243 711
PvuII CAG'CTG
Cuts at: 0 175 548 713 954
Size: 175 373 165 241
Fragments arranged by size:
373 241 175 165
SfcI C'TryA_G
Cuts at: 0 239 954
Size: 239 715
SmlI C'TyrA_G
Cuts at: 0 483 954
Size: 483 471
SspI AAT'ATT
Cuts at: 0 301 954
Size: 301 653
StyI C'CwwG_G
Cuts at: 0 90 250 954
Size: 90 160 704
Fragments arranged by size:
704 160 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 647 954
Size: 647 307
TatI w'GTAC_w
Cuts at: 0 678 954
Size: 678 276
XbaI T'CTAG_A
Cuts at: 0 469 954
Size: 469 485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 163 592 954
Size: 163 429 362
Fragments arranged by size:
429 362 163
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApoI BanI BanII
BciVI BglI Bme1580I BpmI BpuEI BsaI BsaBI BseRI
BseYI BsgI BsmI Bsp1286I BsrFI BtgI EaeI Eco57MI
EcoO109I FalI HaeII Hin4I KpnI MmeI MslI MspA1I
NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SfcI SmlI SspI StyI TaqII TatI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XhoI XmaI
XmnI ZraI