Restriction map for
pFN21AB6362
(Linear) (Six-base) MAPSORT of: KIBB6362.seq Check: 9979 from: 1 to: 873
KIBB6362.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 873
Size: 691 182
AfeI AGC'GCT
Cuts at: 0 259 553 873
Size: 259 294 320
Fragments arranged by size:
320 294 259
AhdI GACnn_n'nnGTC
Cuts at: 0 570 873
Size: 570 303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 873
Size: 586 32 255
Fragments arranged by size:
586 255 32
AlwNI CAG_nnn'CTG
Cuts at: 0 318 873
Size: 318 555
ApaLI G'TGCA_C
Cuts at: 0 137 847 873
Size: 137 710 26
Fragments arranged by size:
710 137 26
AvaI C'yCGr_G
Cuts at: 0 90 873
Size: 90 783
BanII G_rGCy'C
Cuts at: 0 91 338 454 873
Size: 91 247 116 419
Fragments arranged by size:
419 247 116 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 659 873
Size: 431 228 214
Fragments arranged by size:
431 228 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 118 152 873
Size: 118 34 721
Fragments arranged by size:
721 118 34
Bme1580I G_kGCm'C
Cuts at: 0 141 851 873
Size: 141 710 22
Fragments arranged by size:
710 141 22
BmgBI CAC'GTC
Cuts at: 0 215 846 873
Size: 215 631 27
Fragments arranged by size:
631 215 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 873
Size: 141 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 447 873
Size: 383 64 426
Fragments arranged by size:
426 383 64
BsaBI GATnn'nnATC
Cuts at: 0 517 873
Size: 517 356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 586 616 873
Size: 439 30 117 30 257
Fragments arranged by size:
439 257 117 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 591 873
Size: 591 282
BsiEI CG_ry'CG
Cuts at: 0 307 330 873
Size: 307 23 543
Fragments arranged by size:
543 307 23
BsiHKAI G_wGCw'C
Cuts at: 0 91 141 583 851 873
Size: 91 50 442 268 22
Fragments arranged by size:
442 268 91 50 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 465 873
Size: 465 408
Bsp1286I G_dGCh'C
Cuts at: 0 91 141 338 454 583 851 873
Size: 91 50 197 116 129 268 22
Fragments arranged by size:
268 197 129 116 91 50 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 873
Size: 386 487
BsrFI r'CCGG_y
Cuts at: 0 66 436 873
Size: 66 370 437
Fragments arranged by size:
437 370 66
BsrGI T'GTAC_A
Cuts at: 0 702 873
Size: 702 171
BssHII G'CGCG_C
Cuts at: 0 152 369 873
Size: 152 217 504
Fragments arranged by size:
504 217 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 873
Size: 318 555
BtgI C'CryG_G
Cuts at: 0 57 107 330 873
Size: 57 50 223 543
Fragments arranged by size:
543 223 57 50
BtsI GCAGTG_nn'
Cuts at: 0 447 754 849 873
Size: 447 307 95 24
Fragments arranged by size:
447 307 95 24
DraIII CAC_nnn'GTG
Cuts at: 0 477 873
Size: 477 396
EaeI y'GGCC_r
Cuts at: 0 327 873
Size: 327 546
EagI C'GGCC_G
Cuts at: 0 327 873
Size: 327 546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 149 873
Size: 149 724
EcoICRI GAG'CTC
Cuts at: 0 89 873
Size: 89 784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 447 691 873
Size: 383 64 244 182
Fragments arranged by size:
383 244 182 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 761 815 873
Size: 761 54 58
Fragments arranged by size:
761 58 54
EcoO109I rG'GnC_Cy
Cuts at: 0 103 873
Size: 103 770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 795 873
Size: 795 78
FspI TGC'GCA
Cuts at: 0 219 873
Size: 219 654
FspAI rTGC'GCAy
Cuts at: 0 219 873
Size: 219 654
HaeII r_GCGC'y
Cuts at: 0 261 319 391 437 555 873
Size: 261 58 72 46 118 318
Fragments arranged by size:
318 261 118 72 58 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 439 471 556 588 873
Size: 5 32 402 32 85 32 285
Fragments arranged by size:
402 285 85 32 32 32 5
HincII GTy'rAC
Cuts at: 0 345 564 873
Size: 345 219 309
Fragments arranged by size:
345 309 219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 873
Size: 398 475
MspA1I CmG'CkG
Cuts at: 0 6 31 59 309 332 873
Size: 6 25 28 250 23 541
Fragments arranged by size:
541 250 28 25 23 6
NaeI GCC'GGC
Cuts at: 0 438 873
Size: 438 435
NgoMIV G'CCGG_C
Cuts at: 0 436 873
Size: 436 437
NspI r_CATG'y
Cuts at: 0 538 864 873
Size: 538 326 9
Fragments arranged by size:
538 326 9
PasI CC'CwG_GG
Cuts at: 0 695 873
Size: 695 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 23 873
Size: 23 850
PfoI T'CCnGG_A
Cuts at: 0 399 873
Size: 399 474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 873
Size: 586 32 255
Fragments arranged by size:
586 255 32
PpuMI rG'GwC_Cy
Cuts at: 0 103 873
Size: 103 770
PsiI TTA'TAA
Cuts at: 0 737 873
Size: 737 136
PstI C_TGCA'G
Cuts at: 0 314 873
Size: 314 559
SacI G_AGCT'C
Cuts at: 0 91 873
Size: 91 782
SacII CC_GC'GG
Cuts at: 0 60 333 873
Size: 60 273 540
Fragments arranged by size:
540 273 60
SanDI GG'GwC_CC
Cuts at: 0 103 873
Size: 103 770
SfcI C'TryA_G
Cuts at: 0 310 855 873
Size: 310 545 18
Fragments arranged by size:
545 310 18
SgrAI Cr'CCGG_yG
Cuts at: 0 66 873
Size: 66 807
SphI G_CATG'C
Cuts at: 0 538 864 873
Size: 538 326 9
Fragments arranged by size:
538 326 9
StyI C'CwwG_G
Cuts at: 0 830 873
Size: 830 43
TatI w'GTAC_w
Cuts at: 0 702 873
Size: 702 171
Enzymes that do cut:
AcuI AfeI AhdI AloI AlwNI ApaLI AvaI BanII
BbsI BcgI Bme1580I BmgBI BmrI BpmI BsaBI BsaXI
BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI
BssHII BstAPI BtgI BtsI DraIII EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HincII MmeI MspA1I NaeI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PsiI PstI SacI SacII
SanDI SfcI SgrAI SphI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseYI BsgI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI
EcoRI EcoRV FseI HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI