Restriction map for
pFN21AB6361
(Linear) (Six-base) MAPSORT of: KIBB6361.seq Check: 8284 from: 1 to: 507
KIBB6361.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AfeI AGC'GCT
Cuts at: 0 363 507
Size: 363 144
AhdI GACnn_n'nnGTC
Cuts at: 0 35 507
Size: 35 472
AleI CACnn'nnGTG
Cuts at: 0 319 507
Size: 319 188
AvaI C'yCGr_G
Cuts at: 0 147 507
Size: 147 360
BanI G'GyrC_C
Cuts at: 0 223 507
Size: 223 284
BanII G_rGCy'C
Cuts at: 0 92 154 507
Size: 92 62 353
Fragments arranged by size:
353 92 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 507
Size: 485 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 305 507
Size: 305 202
BglI GCCn_nnn'nGGC
Cuts at: 0 439 507
Size: 439 68
BlpI GC'TnA_GC
Cuts at: 0 358 507
Size: 358 149
BmgBI CAC'GTC
Cuts at: 0 335 507
Size: 335 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 507
Size: 373 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 507
Size: 43 464
BseYI C'CCAG_C
Cuts at: 0 106 155 507
Size: 106 49 352
Fragments arranged by size:
352 106 49
BsiEI CG_ry'CG
Cuts at: 0 73 507
Size: 73 434
Bsp1286I G_dGCh'C
Cuts at: 0 92 154 507
Size: 92 62 353
Fragments arranged by size:
353 92 62
BspHI T'CATG_A
Cuts at: 0 207 507
Size: 207 300
BsrFI r'CCGG_y
Cuts at: 0 75 447 507
Size: 75 372 60
Fragments arranged by size:
372 75 60
BssHII G'CGCG_C
Cuts at: 0 16 507
Size: 16 491
BtgI C'CryG_G
Cuts at: 0 7 219 434 507
Size: 7 212 215 73
Fragments arranged by size:
215 212 73 7
EaeI y'GGCC_r
Cuts at: 0 70 125 413 507
Size: 70 55 288 94
Fragments arranged by size:
288 94 70 55
EagI C'GGCC_G
Cuts at: 0 70 507
Size: 70 437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 258 507
Size: 258 249
EcoNI CCTnn'n_nnAGG
Cuts at: 0 182 507
Size: 182 325
HaeII r_GCGC'y
Cuts at: 0 49 365 507
Size: 49 316 142
Fragments arranged by size:
316 142 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 189 221 507
Size: 21 32 136 32 286
Fragments arranged by size:
286 136 32 32 21
MscI TGG'CCA
Cuts at: 0 415 507
Size: 415 92
MslI CAynn'nnrTG
Cuts at: 0 319 507
Size: 319 188
MspA1I CmG'CkG
Cuts at: 0 9 124 159 411 507
Size: 9 115 35 252 96
Fragments arranged by size:
252 115 96 35 9
NaeI GCC'GGC
Cuts at: 0 77 507
Size: 77 430
NcoI C'CATG_G
Cuts at: 0 434 507
Size: 434 73
NgoMIV G'CCGG_C
Cuts at: 0 75 507
Size: 75 432
NspI r_CATG'y
Cuts at: 0 383 507
Size: 383 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 251 507
Size: 251 256
PvuII CAG'CTG
Cuts at: 0 159 507
Size: 159 348
SacII CC_GC'GG
Cuts at: 0 10 507
Size: 10 497
SgrAI Cr'CCGG_yG
Cuts at: 0 75 507
Size: 75 432
SmaI CCC'GGG
Cuts at: 0 149 507
Size: 149 358
SmlI C'TyrA_G
Cuts at: 0 388 507
Size: 388 119
SphI G_CATG'C
Cuts at: 0 383 507
Size: 383 124
StyI C'CwwG_G
Cuts at: 0 434 507
Size: 434 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 507
Size: 25 482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 507
Size: 423 84
XmaI C'CCGG_G
Cuts at: 0 147 507
Size: 147 360
Enzymes that do cut:
AfeI AhdI AleI AvaI BanI BanII BbsI BciVI
BglI BlpI BmgBI BpuEI BsaI BseYI BsiEI Bsp1286I
BspHI BsrFI BssHII BtgI EaeI EagI EciI EcoNI
HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PvuII SacII SgrAI SmaI SmlI SphI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BfrBI BglII Bme1580I BmrI BmtI BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SnaBI SpeI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI