Restriction map for
pFN21AB6323
(Linear) (Six-base) MAPSORT of: KIBB6323.seq Check: 9409 from: 1 to: 783
KIBB6323.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 105 460 783
Size: 105 355 323
Fragments arranged by size:
355 323 105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 337 496 783
Size: 269 68 159 287
Fragments arranged by size:
287 269 159 68
AflIII A'CryG_T
Cuts at: 0 545 783
Size: 545 238
AleI CACnn'nnGTG
Cuts at: 0 733 783
Size: 733 50
AlwNI CAG_nnn'CTG
Cuts at: 0 385 783
Size: 385 398
ApaI G_GGCC'C
Cuts at: 0 691 783
Size: 691 92
ApaLI G'TGCA_C
Cuts at: 0 39 163 783
Size: 39 124 620
Fragments arranged by size:
620 124 39
BanI G'GyrC_C
Cuts at: 0 46 783
Size: 46 737
BanII G_rGCy'C
Cuts at: 0 602 691 783
Size: 602 89 92
Fragments arranged by size:
602 92 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 257 350 502 783
Size: 257 93 152 281
Fragments arranged by size:
281 257 152 93
Bme1580I G_kGCm'C
Cuts at: 0 43 56 144 167 691 705 783
Size: 43 13 88 23 524 14 78
Fragments arranged by size:
524 88 78 43 23 14 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 451 783
Size: 165 286 332
Fragments arranged by size:
332 286 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 783
Size: 333 450
Bpu10I CC'TnA_GC
Cuts at: 0 692 783
Size: 692 91
BsaWI w'CCGG_w
Cuts at: 0 746 783
Size: 746 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 783
Size: 502 281
BseYI C'CCAG_C
Cuts at: 0 83 222 467 595 783
Size: 83 139 245 128 188
Fragments arranged by size:
245 188 139 128 83
BsiHKAI G_wGCw'C
Cuts at: 0 43 167 182 783
Size: 43 124 15 601
Fragments arranged by size:
601 124 43 15
BsmI GAATG_Cn'
Cuts at: 0 99 783
Size: 99 684
Bsp1286I G_dGCh'C
Cuts at: 0 43 56 144 167 182 602 691 705
Size: 43 13 88 23 15 420 89 14
Cuts at: 705 783
Size: 78
Fragments arranged by size:
420 89 88 78 43 23 15 14
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 460 783
Size: 105 355 323
Fragments arranged by size:
355 323 105
BsrBI CCG'CTC
Cuts at: 0 297 783
Size: 297 486
BsrDI GCAATG_nn'
Cuts at: 0 192 783
Size: 192 591
BsrGI T'GTAC_A
Cuts at: 0 125 725 783
Size: 125 600 58
Fragments arranged by size:
600 125 58
Bsu36I CC'TnA_GG
Cuts at: 0 462 783
Size: 462 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6 753 783
Size: 6 747 30
Fragments arranged by size:
747 30 6
DraIII CAC_nnn'GTG
Cuts at: 0 148 783
Size: 148 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 333 337 496 783
Size: 269 64 4 159 287
Fragments arranged by size:
287 269 159 64 4
EcoO109I rG'GnC_Cy
Cuts at: 0 6 267 411 687 688 783
Size: 6 261 144 276 1 95
Fragments arranged by size:
276 261 144 95 6 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 513 545 783
Size: 331 32 150 32 238
Fragments arranged by size:
331 238 150 32 32
MslI CAynn'nnrTG
Cuts at: 0 733 783
Size: 733 50
MspA1I CmG'CkG
Cuts at: 0 187 783
Size: 187 596
NspI r_CATG'y
Cuts at: 0 549 783
Size: 549 234
PciI A'CATG_T
Cuts at: 0 545 783
Size: 545 238
PpuMI rG'GwC_Cy
Cuts at: 0 6 267 411 783
Size: 6 261 144 372
Fragments arranged by size:
372 261 144 6
PspOMI G'GGCC_C
Cuts at: 0 687 783
Size: 687 96
PvuII CAG'CTG
Cuts at: 0 187 783
Size: 187 596
SanDI GG'GwC_CC
Cuts at: 0 267 783
Size: 267 516
SexAI A'CCwGG_T
Cuts at: 0 8 783
Size: 8 775
StyI C'CwwG_G
Cuts at: 0 731 783
Size: 731 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 442 783
Size: 165 277 341
Fragments arranged by size:
341 277 165
TatI w'GTAC_w
Cuts at: 0 125 725 783
Size: 125 600 58
Fragments arranged by size:
600 125 58
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI BanI
BanII BbsI Bme1580I BmrI BpmI Bpu10I BsaWI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI
Bsu36I BtgZI DraIII Eco57MI EcoO109I Hin4I MslI MspA1I
NspI PciI PpuMI PspOMI PvuII SanDI SexAI StyI
TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI