Restriction map for
pFN21AB6296
(Linear) (Six-base) MAPSORT of: KIBB6296.seq Check: 8061 from: 1 to: 2376
KIBB6296.orf
With 182 enzymes: SgfI *
July 3, 2012 16:20 ..
AccI GT'mk_AC
Cuts at: 0 152 1141 2376
Size: 152 989 1235
Fragments arranged by size:
1235 989 152
Acc65I G'GTAC_C
Cuts at: 0 241 2376
Size: 241 2135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1193 1457 1682 2376
Size: 1193 264 225 694
Fragments arranged by size:
1193 694 264 225
AflII C'TTAA_G
Cuts at: 0 1912 2209 2376
Size: 1912 297 167
Fragments arranged by size:
1912 297 167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1506 1538 2376
Size: 1506 32 838
Fragments arranged by size:
1506 838 32
ApoI r'AATT_y
Cuts at: 0 924 1805 2005 2054 2376
Size: 924 881 200 49 322
Fragments arranged by size:
924 881 322 200 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 881 914 1520 1553 2376
Size: 881 33 606 33 823
Fragments arranged by size:
881 823 606 33 33
BanI G'GyrC_C
Cuts at: 0 241 1074 2376
Size: 241 833 1302
Fragments arranged by size:
1302 833 241
BanII G_rGCy'C
Cuts at: 0 2040 2376
Size: 2040 336
BciVI GTATCCnnnnn_n'
Cuts at: 0 867 1548 1936 2086 2376
Size: 867 681 388 150 290
Fragments arranged by size:
867 681 388 290 150
BclI T'GATC_A
Cuts at: 0 104 1094 2376
Size: 104 990 1282
Fragments arranged by size:
1282 990 104
BglI GCCn_nnn'nGGC
Cuts at: 0 2140 2376
Size: 2140 236
BglII A'GATC_T
Cuts at: 0 1923 2376
Size: 1923 453
BmgBI CAC'GTC
Cuts at: 0 150 2376
Size: 150 2226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 191 2054 2376
Size: 45 146 1863 322
Fragments arranged by size:
1863 322 146 45
Bpu10I CC'TnA_GC
Cuts at: 0 1447 2376
Size: 1447 929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 2376
Size: 1067 1309
BsaBI GATnn'nnATC
Cuts at: 0 106 2376
Size: 106 2270
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 209 2376
Size: 7 202 2167
Fragments arranged by size:
2167 202 7
BseYI C'CCAG_C
Cuts at: 0 10 1574 2233 2376
Size: 10 1564 659 143
Fragments arranged by size:
1564 659 143 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 2376
Size: 130 2246
BsiHKAI G_wGCw'C
Cuts at: 0 651 2040 2376
Size: 651 1389 336
Fragments arranged by size:
1389 651 336
BsmI GAATG_Cn'
Cuts at: 0 533 2376
Size: 533 1843
Bsp1286I G_dGCh'C
Cuts at: 0 651 2040 2376
Size: 651 1389 336
Fragments arranged by size:
1389 651 336
BspHI T'CATG_A
Cuts at: 0 825 2189 2376
Size: 825 1364 187
Fragments arranged by size:
1364 825 187
BsrBI CCG'CTC
Cuts at: 0 82 2376
Size: 82 2294
BsrDI GCAATG_nn'
Cuts at: 0 297 2376
Size: 297 2079
BsrFI r'CCGG_y
Cuts at: 0 51 1071 2376
Size: 51 1020 1305
Fragments arranged by size:
1305 1020 51
BsrGI T'GTAC_A
Cuts at: 0 770 2376
Size: 770 1606
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1411 1687 2376
Size: 1411 276 689
Fragments arranged by size:
1411 689 276
BstEII G'GTnAC_C
Cuts at: 0 1569 2376
Size: 1569 807
BstYI r'GATC_y
Cuts at: 0 1041 1923 2376
Size: 1041 882 453
Fragments arranged by size:
1041 882 453
BtgI C'CryG_G
Cuts at: 0 222 2376
Size: 222 2154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 2376
Size: 117 2259
BtsI GCAGTG_nn'
Cuts at: 0 1681 2376
Size: 1681 695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1742 1777 1956 1991 2320 2355 2376
Size: 1742 35 179 35 329 35 21
Fragments arranged by size:
1742 329 179 35 35 35 21
DraI TTT'AAA
Cuts at: 0 479 1362 1812 2010 2372 2376
Size: 479 883 450 198 362 4
Fragments arranged by size:
883 479 450 362 198 4
EaeI y'GGCC_r
Cuts at: 0 86 948 1763 2376
Size: 86 862 815 613
Fragments arranged by size:
862 815 613 86
EarI CTCTTCn'nnn_
Cuts at: 0 990 2376
Size: 990 1386
EcoICRI GAG'CTC
Cuts at: 0 2038 2376
Size: 2038 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 191 1193 1457 1682 2054 2376
Size: 45 146 1002 264 225 372 322
Fragments arranged by size:
1002 372 322 264 225 146 45
EcoO109I rG'GnC_Cy
Cuts at: 0 789 1462 1984 2376
Size: 789 673 522 392
Fragments arranged by size:
789 673 522 392
EcoRV GAT'ATC
Cuts at: 0 201 2294 2376
Size: 201 2093 82
Fragments arranged by size:
2093 201 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 807 839 1123 1155 1230 1262
Size: 185 32 590 32 284 32 75 32
Cuts at: 1262 2251 2283 2376
Size: 989 32 93
Fragments arranged by size:
989 590 284 185 93 75 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2179 2376
Size: 2179 197
KpnI G_GTAC'C
Cuts at: 0 245 2376
Size: 245 2131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 508 2122 2376
Size: 476 32 1614 254
Fragments arranged by size:
1614 476 254 32
MscI TGG'CCA
Cuts at: 0 88 950 1765 2376
Size: 88 862 815 611
Fragments arranged by size:
862 815 611 88
MslI CAynn'nnrTG
Cuts at: 0 538 830 2376
Size: 538 292 1546
Fragments arranged by size:
1546 538 292
MspA1I CmG'CkG
Cuts at: 0 187 583 1092 2376
Size: 187 396 509 1284
Fragments arranged by size:
1284 509 396 187
NcoI C'CATG_G
Cuts at: 0 222 2376
Size: 222 2154
NdeI CA'TA_TG
Cuts at: 0 1677 2376
Size: 1677 699
NspI r_CATG'y
Cuts at: 0 1720 2376
Size: 1720 656
PflMI CCAn_nnn'nTGG
Cuts at: 0 946 2376
Size: 946 1430
PfoI T'CCnGG_A
Cuts at: 0 92 2031 2376
Size: 92 1939 345
Fragments arranged by size:
1939 345 92
PpuMI rG'GwC_Cy
Cuts at: 0 789 1462 1984 2376
Size: 789 673 522 392
Fragments arranged by size:
789 673 522 392
PstI C_TGCA'G
Cuts at: 0 2049 2376
Size: 2049 327
PvuII CAG'CTG
Cuts at: 0 187 583 1092 2376
Size: 187 396 509 1284
Fragments arranged by size:
1284 509 396 187
SacI G_AGCT'C
Cuts at: 0 2040 2376
Size: 2040 336
SanDI GG'GwC_CC
Cuts at: 0 789 2376
Size: 789 1587
ScaI AGT'ACT
Cuts at: 0 1545 2376
Size: 1545 831
SfcI C'TryA_G
Cuts at: 0 502 1142 1721 1902 2045 2376
Size: 502 640 579 181 143 331
Fragments arranged by size:
640 579 502 331 181 143
SmlI C'TyrA_G
Cuts at: 0 1082 1912 2209 2376
Size: 1082 830 297 167
Fragments arranged by size:
1082 830 297 167
SspI AAT'ATT
Cuts at: 0 251 2118 2128 2376
Size: 251 1867 10 248
Fragments arranged by size:
1867 251 248 10
StuI AGG'CCT
Cuts at: 0 2274 2376
Size: 2274 102
StyI C'CwwG_G
Cuts at: 0 222 2376
Size: 222 2154
SwaI ATTT'AAAT
Cuts at: 0 479 2376
Size: 479 1897
TatI w'GTAC_w
Cuts at: 0 770 1086 1171 1543 1603 2376
Size: 770 316 85 372 60 773
Fragments arranged by size:
773 770 372 316 85 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2187 2219 2376
Size: 2187 32 157
Fragments arranged by size:
2187 157 32
Enzymes that do cut:
AccI Acc65I AcuI AflII AloI ApoI BaeI BanI
BanII BciVI BclI BglI BglII BmgBI BpmI Bpu10I
BpuEI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRV Hin4I HincII KpnI MmeI MscI
MslI MspA1I NcoI NdeI NspI PflMI PfoI PpuMI
PstI PvuII SacI SanDI ScaI SfcI SmlI SspI
StuI StyI SwaI TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbsI BbvCI BcgI BfrBI BlpI
Bme1580I BmrI BmtI BplI BsaI BsaAI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspMI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I ClaI DraIII DrdI EagI
EciI EcoNI EcoRI FalI FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI